公开(公告)号：US20110195505A1

公开(公告)日：2011-08-11

申请号：US12899760

申请日：2010-10-07

申请人： LORI JEAN EULER ,  DENNIS FLINT ,  BRIAN JAMES PAUL ,  TINA K. VAN DYK ,  RICK W. YE

发明人： LORI JEAN EULER ,  DENNIS FLINT ,  BRIAN JAMES PAUL ,  TINA K. VAN DYK ,  RICK W. YE

IPC分类号： C12N15/74 ,  C12N1/21 ,  C12N1/36

CPC分类号： C12P7/16 ,  C07K14/335 ,  C12N1/36 ,  Y02E50/10

摘要： Bacteria that are not natural butanol producers were found to have increased tolerance to butanol when the saturated fatty acids content in bacterial cell membrane was increased. Methods for increasing the concentration of saturated fatty acids in the membranes of bacteria that are not natural butanol produces are described whereby tolerance of the bacterial cell to butanol is increased. Saturated fatty acids concentration in the bacterial cell membrane increased upon exogenously feeding saturated fatty acids to cells. Bacterial strains useful for production of butanol are described herein having modified unsaturated fatty acid biosynthetic pathway.

摘要翻译： 当细菌细胞膜中的饱和脂肪酸含量增加时，发现不是天然丁醇生产者的细菌对丁醇具有增加的耐受性。 描述了增加非天然丁醇产生的细菌膜中饱和脂肪酸浓度的方法，其中细菌细胞对丁醇的耐受性增加。 饱和脂肪酸在细胞细胞膜中的浓度在将饱和脂肪酸外源供给细胞后增加。 本文描述了可用于生产丁醇的细菌菌株，其具有改性的不饱和脂肪酸生物合成途径。

公开(公告)号：US20100136641A1

公开(公告)日：2010-06-03

申请号：US12625593

申请日：2009-11-25

申请人： DENNIS FLINT ,  TINA K. VAN DYK

发明人： DENNIS FLINT ,  TINA K. VAN DYK

IPC分类号： C12P7/16 ,  C12N1/20 ,  C12N15/74

CPC分类号： C12P7/16 ,  C12N1/38 ,  C12N9/90 ,  Y02E50/10

摘要： Bacteria that are not natural butanol producers were found to have increased tolerance to butanol when the membrane content of unsaturated trans fatty acids was increased. Feeding cells with unsaturated trans fatty acids increased their concentration in the membrane, which may also be accomplished by expressing a fatty acid cistrans isomerase.

摘要翻译： 发现当不饱和反式脂肪酸的膜含量增加时，不是天然丁醇生产者的细菌具有增加的对丁醇的耐受性。 用不饱和反式脂肪酸喂养细胞增加其在膜中的浓度，这也可以通过表达脂肪酸顺反异构酶来实现。

公开(公告)号：US20100221801A1

公开(公告)日：2010-09-02

申请号：US12711308

申请日：2010-02-24

申请人： TINA K. VAN DYK

发明人： TINA K. VAN DYK

IPC分类号： C12P7/16 ,  C12N1/19

CPC分类号： C12P7/16 ,  Y02E50/10

摘要： Increasing tolerance to butanol in yeast has been accomplished by decreasing activity of Pdr5p encoded by an endogenous PDR5 gene. A deletion mutation of the PDR5 gene led to improved growth yield in the presence of butanol. Yeast cells with reduced Pdr5p activity, or other multidrug resistance ATP-binding cassette transporter protein activity encoded by CDR1 or BFR1, and a butanol biosynthetic pathway may be used for improved butanol production

摘要翻译： 通过降低由内源性PDR5基因编码的Pdr5p的活性，可以增加对酵母中丁醇的耐受性。 PDR5基因的缺失突变导致丁醇存在下生长产量提高。 具有降低的Pdr5p活性的酵母细胞或由CDR1或BFR1编码的其他多药耐药性ATP结合盒转运蛋白活性和丁醇生物合成途径可用于改善丁醇生产

公开(公告)号：US20130230892A1

公开(公告)日：2013-09-05

申请号：US13412124

申请日：2012-03-05

申请人： DANA MICHELLE WALTERS POLLAK ,  TINA K. VAN DYK

发明人： DANA MICHELLE WALTERS POLLAK ,  TINA K. VAN DYK

IPC分类号： C07K14/00 ,  C12P7/42 ,  C12P7/18 ,  C12N1/21 ,  C12P7/20

CPC分类号： C07K14/195 ,  C07K14/245 ,  C12P7/18 ,  C12P7/20 ,  C12P7/42

摘要： Variant sucrose transporter polypeptides that enable bacterial growth over a wide range of gene expression levels and sucrose concentrations are described. Additionally, recombinant bacteria comprising these variant sucrose transporter polypeptides, and methods of utilizing the bacteria to produce products such as glycerol and glycerol-derived products are provided

摘要翻译： 描述了能够在广泛的基因表达水平和蔗糖浓度下进行细菌生长的变体蔗糖转运蛋白多肽。 此外，提供了包含这些变体蔗糖转运蛋白多肽的重组细菌，以及利用细菌产生产物如甘油和甘油衍生产物的方法

公开(公告)号：US20120142064A1

公开(公告)日：2012-06-07

申请号：US12960634

申请日：2010-12-06

申请人： TINA K. VAN DYK

发明人： TINA K. VAN DYK

IPC分类号： C12P7/18 ,  C12P7/52 ,  C12P7/20 ,  C12N1/21 ,  C12N1/20

CPC分类号： C12P7/18 ,  C12N15/52 ,  C12P7/20 ,  C12P7/42

摘要： Recombinant bacteria capable of metabolizing sucrose are described. The recombinant bacteria comprise in their genome or on at least one recombinant construct: a nucleotide sequence from Bacillus licheniformis ATCC® 14580 encoding a polypeptide having sucrose transporter activity and a nucleotide sequence from Bacillus licheniformis ATCC® 14580 encoding a polypeptide having sucrose hydrolase activity. These nucleotide sequences are each operably linked to the same or a different promoter. Recombinant bacteria capable of metabolizing sucrose to produce glycerol and/or glycerol-derived products such as 1,3-propanediol and 3-hydroxypropionic acid are also described.

摘要翻译： 描述了能够代谢蔗糖的重组细菌。 重组细菌在其基因组或至少一种重组构建体中包含：编码具有蔗糖转运蛋白活性的多肽的地衣芽孢杆菌ATCC 14580的核苷酸序列和编码具有蔗糖水解酶活性的多肽的地衣芽孢杆菌ATCC 14580的核苷酸序列。 这些核苷酸序列各自可操作地连接到相同或不同的启动子。 还描述了能够代谢蔗糖以产生甘油和/或甘油衍生产物如1,3-丙二醇和3-羟基丙酸的重组细菌。