公开(公告)号：US11424114B2

公开(公告)日：2022-08-23

申请号：US16767760

申请日：2018-11-19

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： John Lawrence Campbell ,  Scott Hopkins

IPC: H01J49/00 ,  G06N20/20 ,  G01N27/62 ,  G01N27/623

Abstract: A plurality of known compounds with known CCS values is analyzed using a DMS device. The DMS device determines how the intensities of their transmitted ions vary with different separation voltages (SVs) and compensation voltages (CVs). A machine learning algorithm builds a data model from the known m/z value, known CCS value, and measured pairs of CV and SV values that provide optimal transmission through the DMS device for each of the known compounds. An unknown compound with an unknown CCS value is then analyzed. The DMS device determines how the intensity of its ions varies with the same different SVs and CVs. Finally, the machine learning algorithm predicts the CCS value of the unknown compound from the data model, the known m/z of the unknown compound, and the measured pairs of CV and SV values that provide optimal transmission through the DMS device for the unknown compound.

公开(公告)号：US20190137447A1

公开(公告)日：2019-05-09

申请号：US16093681

申请日：2017-04-12

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： John Lawrence Campbell

IPC: G01N27/62 ,  H01J49/04 ,  H01J49/10 ,  C07B63/00

Abstract: Methods and systems for separating, detecting, and/or quantifying sample enantiomers using differential mobility spectrometry (DMS) are provided herein. In accordance with various aspects of the applicant's teachings, the methods and systems can provide for the separation of racemic or non-racemic mixtures of sample enantiomers that may be difficult to separate with conventional techniques, such as mass spectrometry (MS), by reacting the sample enantiomers with an enantiomerically-pure compound to form diastereomers separable by DMS.

公开(公告)号：US10128093B2

公开(公告)日：2018-11-13

申请号：US15576874

申请日：2016-05-26

Applicant: DH Technologies Development Pte. Ltd.

Inventor： Stephen A. Tate ,  John Lawrence Campbell

IPC: H01J49/00

Abstract: Systems and methods prevent potentially convolved precursor ion peaks from being excluded in subsequent cycles of an IDA experiment so that additional product ion data is collected. A sample is ionized producing an ion beam. A plurality of cycles of an IDA experiment are performed on the ion beam. During each cycle of the IDA experiment and for each precursor ion peak on a filtered peak list produced in the filtering step of each cycle, several steps are performed. The precursor ion peak is identified in the precursor ion spectrum produced in the MS survey scan step of the cycle. It is determined if the precursor ion peak in the precursor ion spectrum includes a feature of convolution. If the precursor ion peak includes a feature of convolution, the precursor ion peak is prevented from being excluded in a filtering step of one or more subsequent cycles.

公开(公告)号：US20170117124A1

公开(公告)日：2017-04-27

申请号：US15316915

申请日：2015-06-04

Applicant: DH Technologies Development PTE Ltd.

Inventor： Takashi Baba ,  John Lawrence Campbell

IPC: H01J49/00 ,  H01J49/42

CPC classification number: H01J49/0054 ,  G01N33/92 ,  H01J49/0072 ,  H01J49/4225

Abstract: A method and apparatus for analyzing samples using mass spectrometry are disclosed. The apparatus includes a reaction device configured to dissociate sample ions into fragments by reacting the sample ions with a charged species (e.g., electrons) such as through ECD, EID, or EIEIO. The kinetic energy of the charged species is such that the fragments may be detected and produce spectra that allow for the determination of isomeric species in the sample and the location of double bonds of sample molecules. The fragments may include radical fragments and non-radical fragments. The apparatus may also include an oxygen gas source configured to react with the radical fragments to produce oxygen-radical fragments. Spectra resulting from analysis of the fragments may allow for the determination of the oxygen-radical fragments resulting from the dissociation of the sample molecules.

公开(公告)号：US20150332904A1

公开(公告)日：2015-11-19

申请号：US14443933

申请日：2013-11-21

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： John Lawrence Campbell

IPC: H01J49/00

CPC classification number: H01J49/005 ,  H01J49/0031

Abstract: Systems and methods are provided for reducing the time period of a CID event of an MS3 experiment and making the overall fragmentation event more generic. A CID event of an MS3 experiment performed on a sample by a mass spectrometer is divided into two time periods using a processor. At the beginning of a first time period of the CID event, the mass spectrometer is instructed to both open a pulse valve in order to pulse a collision gas and apply a first CID voltage. At the beginning of a second time period of the CID event, the mass spectrometer is instructed to both close the pulse valve and apply a second CID voltage. The mass spectrometer is pumped down during the second time period. The overlap in time of the pump down and CID reduces the overall time period of the CID event.

Abstract translation: 提供了系统和方法来减少MS3实验的CID事件的时间周期，并使整个碎片事件更为通用。 通过质谱仪对样品进行的MS3实验的CID事件使用处理器分成两个时间段。 在CID事件的第一时间段开始时，指示质谱仪打开脉冲阀以便脉冲碰撞气体并施加第一CID电压。 在CID事件的第二时间段开始时，指示质谱仪关闭脉冲阀并施加第二CID电压。 质谱仪在第二时间内被抽出。 泵送时间和CID的重叠减少了CID事件的总体时间。

公开(公告)号：US20140264009A1

公开(公告)日：2014-09-18

申请号：US14361378

申请日：2012-11-21

Applicant: DH Technologies Development Pte. Ltd.

Inventor： John Lawrence Campbell ,  Yves LeBlanc

IPC: G01N27/62

CPC classification number: G01N27/624 ,  G01N27/622 ,  G01N33/6848 ,  H01J49/0072

Abstract: An apparatus and method are provided for analyzing samples of molecules. The apparatus comprises a mass analysis system including a differential mobility spectrometer, which includes at least three filter electrodes defining two ion flow paths where the filter electrodes generate electric fields for passing through selected portions of the sample ions based on the mobility characteristics of the sample ions. The differential mobility spectrometer also includes a voltage source that provides DC and RF voltages to at least one of the filter electrodes to generate the electric field, a first and a second ion inlet that receive sample ions, and an ion outlet that outputs the selected portion of the sample ions. A mass spectrometer receives some or all of the selected portion of the sample ions.

Abstract translation: 提供了用于分析分子样品的装置和方法。 该装置包括质量分析系统，其包括差动迁移谱仪，其包括限定两个离子流动路径的至少三个过滤器电极，其中过滤电极基于样品离子的迁移率特性产生用于穿过样本离子的选定部分的电场 。 差动迁移谱仪还包括电压源，其向至少一个滤波电极提供DC和RF电压以产生电场;接收样品离子的第一和第二离子入口;以及输出所选部分的离子出口 的样品离子。 质谱仪接收样品离子的部分或全部选定部分。

公开(公告)号：US20230084101A1

公开(公告)日：2023-03-16

申请号：US17758646

申请日：2021-01-14

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： John Lawrence Campbell ,  Brendon Seale

IPC: G01N27/624 ,  G01N27/623

Abstract: A DMS device receives a curtain gas that includes a chemical modifier into its curtain plate. Before or while receiving ions of an analyte, the DMS device steps the CoV through a series of values in order to apply different CoV values to at least one precursor ion derived from the chemical modifier. For each CoV value of the series of values, a mass spectrometer selects and mass analyzes the at least one precursor ion. An intensity is produced for each CoV value of the series of values. An intensity versus CoV value peak is calculated from the intensities measured. A representative CoV value is calculated for the peak. The difference between the representative CoV value and known CoV values that represent an uncontaminated curtain plate is calculated. If the difference is greater than or equal to a predetermined threshold value, the curtain plate is determined to be contaminated.

公开(公告)号：US10309930B2

公开(公告)日：2019-06-04

申请号：US15516387

申请日：2015-09-21

Applicant: DH Technologies Development Pte. Ltd.

Inventor： John Lawrence Campbell ,  Eva Duchoslav ,  Yves Le Blanc ,  David M. Cox

IPC: H01J49/26 ,  G01N27/62 ,  H01J49/00

Abstract: Systems and methods are provided for providing a DMS precursor ion survey scan. An ion source configured to receive a sample is instructed to ionize the sample using a processor. A DMS device configured to receive ions from the ion source is instructed to separate precursor ions received from the ion source and transmit precursor ions using two or more CoVs using the processor. A mass analyzer configured to receive transmitted precursor ions from the DMS device is instructed to measure the m/z intensities of the transmitted precursor ions across an m/z range at each CoV of the two or more CoVs using the processor. The measured m/z intensities of the transmitted precursor ions received from the mass analyzer are stored as a function of m/z value and CoV using the processor. This produces a stored two-dimensional mapping of m/z intensities of the precursor ions of the sample.

公开(公告)号：US10032614B2

公开(公告)日：2018-07-24

申请号：US15316915

申请日：2015-06-04

Applicant: DH Technologies Development PTE Ltd.

Inventor： Takashi Baba ,  John Lawrence Campbell

IPC: G01N33/92 ,  H01J49/00 ,  H01J49/42

Abstract: A method and apparatus for analyzing samples using mass spectrometry are disclosed. The apparatus includes a reaction device configured to dissociate sample ions into fragments by reacting the sample ions with a charged species (e.g., electrons) such as through ECD, EID, or EIEIO. The kinetic energy of the charged species is such that the fragments may be detected and produce spectra that allow for the determination of isomeric species in the sample and the location of double bonds of sample molecules. The fragments may include radical fragments and non-radical fragments. The apparatus may also include an oxygen gas source configured to react with the radical fragments to produce oxygen-radical fragments. Spectra resulting from analysis of the fragments may allow for the determination of the oxygen-radical fragments resulting from the dissociation of the sample molecules.

公开(公告)号：US09343277B2

公开(公告)日：2016-05-17

申请号：US14443933

申请日：2013-11-21

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： John Lawrence Campbell

IPC: H01J49/00

CPC classification number: H01J49/005 ,  H01J49/0031

Abstract: Systems and methods are provided for reducing the time period of a CID event of an MS3 experiment and making the overall fragmentation event more generic. A CID event of an MS3 experiment performed on a sample by a mass spectrometer is divided into two time periods using a processor. At the beginning of a first time period of the CID event, the mass spectrometer is instructed to both open a pulse valve in order to pulse a collision gas and apply a first CID voltage. At the beginning of a second time period of the CID event, the mass spectrometer is instructed to both close the pulse valve and apply a second CID voltage. The mass spectrometer is pumped down during the second time period. The overlap in time of the pump down and CID reduces the overall time period of the CID event.

Abstract translation: 提供了系统和方法来减少MS3实验的CID事件的时间周期，并使整个碎片事件更为通用。 通过质谱仪对样品进行的MS3实验的CID事件使用处理器分成两个时间段。 在CID事件的第一时间段开始时，指示质谱仪打开脉冲阀以便脉冲碰撞气体并施加第一CID电压。 在CID事件的第二时间段开始时，指示质谱仪关闭脉冲阀并施加第二CID电压。 质谱仪在第二时间内被抽出。 泵送时间和CID的重叠减少了CID事件的总体时间。