公开(公告)号：US20150316507A1

公开(公告)日：2015-11-05

申请号：US14655263

申请日：2013-12-13

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： John L. CAMPBELL ,  Yves LEBLANC

IPC: G01N27/62 ,  H01J49/04 ,  H01J49/00

CPC classification number: G01N27/624 ,  G01N27/622 ,  G01N33/92 ,  G01N2458/15 ,  H01J49/0031 ,  H01J49/0422 ,  Y10T436/24

Abstract: A method for analyzing a sample that contains a plurality of lipid isomers is described that involves forming one or more lipid metal ion adducts and transporting the one or more lipid metal ion adducts through a Mix of POPC+OPPC differential mobility spectrometer to cause separation of the one or more lipid metal ion adducts from each other. The lipid isomers can be chosen, for example, from fatty acids, glycerolipids, glycerophospholipids, sphingolipids, saccharolipids, polyketides, sterol lipids, and prenol lipids. Particular examples include phosphatidylcholine regioisomers such as 1-palmatoyl-2-oleoyl-sn-phosphatidylcholine (POPC) and 1-oleoyl-2-palmatoyl-sn-phosphatidylcholine and triacylglycerols containg palmetic and oleic acid groups. The metal chosen can include a cationization reagent that contains sodium, potassium, silver or lithium.

Abstract translation: 描述了分析含有多种脂质异构体的样品的方法，其涉及形成一种或多种脂质金属离子加合物，并通过POPC + OPPC差速迁移谱仪的混合物输送一种或多种脂质金属离子加合物，以使 一种或多种彼此之间的脂质金属离子加成物。 脂质异构体可以选自例如脂肪酸，甘油脂，甘油磷脂，鞘脂，蔗糖脂，聚酮化合物，甾醇脂质和牛油醇脂质。 具体实例包括磷脂酰胆碱区域异构体，例如1-棕榈酰-2-油酰-sn-磷脂酰胆碱（POPC）和1-油酰-2-棕榈酰-sn-磷脂酰胆碱和含有油酸和油酸基团的三酰基甘油。 所选择的金属可以包括含有钠，钾，银或锂的阳离子化试剂。

公开(公告)号：US20170072397A1

公开(公告)日：2017-03-16

申请号：US15309275

申请日：2015-05-06

Applicant: DH Technologies Development PTE Ltd.

Inventor： John Lawrence CAMPBELL ,  Kihwan CHOI ,  Yves LEBLANC ,  Chang LIU ,  Aaron WHEELER

IPC: B01L3/00 ,  G01N35/00 ,  G01N30/16

CPC classification number: B01L3/502792 ,  B01F13/0071 ,  B01F13/0076 ,  B01L2300/0645 ,  B01L2300/0887 ,  B01L2400/0415 ,  B01L2400/0427 ,  G01N27/44791 ,  G01N30/16 ,  G01N35/00871 ,  G01N2030/162

Abstract: Methods and systems for processing fluids utilizing a digital microfluidic device and transferring droplets from the digital microfluidic device to a downstream analyzer are described herein. Methods and systems in accordance with the present teachings can allow for the withdrawal of fluid from a digital microfluidic device, and can in some aspects enable the integration of a digital microfluidic device as a direct, in-line sample processing platform from which a droplet can be transferred to a downstream analyzer.

Abstract translation: 本文描述了利用数字微流体装置处理流体并将液滴从数字微流体装置转移到下游分析器的方法和系统。 根据本教导的方法和系统可以允许从数字微流体装置中取出流体，并且在某些方面，可以将数字微流体装置整合为直接的在线样品处理平台，液滴可从该样品处理平台 转移到下游分析仪。