公开(公告)号：US20220359178A1

公开(公告)日：2022-11-10

申请号：US17753036

申请日：2020-08-22

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： Yves Le Blanc

IPC: H01J49/00 ,  H01J49/10

Abstract: Before each sample of a series of batch samples is introduced into a liquid sample delivery device, an ion source device receives aqueous mobile phase solution from the liquid sample delivery device and ionizes its compounds, producing an ion beam. A tandem mass spectrometer performs a neutral loss or precursor ion scan on the ion beam to measure intensities of two or more precursor ions corresponding to a known aqueous mobile phase solution compound. Intensity measurements for each of the two or more different precursor ions are compared to previously stored intensities to determine the threshold times at which these measurements indicate orifice contamination. A threshold time is then predicted for a known compound of interest of the batch samples based on the m/z value of the known compound of interest and the m/z value and the threshold time of each of the two or more different precursor ions.

公开(公告)号：US11024494B2

公开(公告)日：2021-06-01

申请号：US16646674

申请日：2018-09-25

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： Yves Le Blanc

IPC: H01J49/00 ,  H01J49/42

Abstract: An interference in a first MRM transition measurement for a compound of interest is determined by using a second MRM transition that includes an isotope of the precursor ion in the first MRM transition. Both transitions include the same product ion. A first intensity is measured for the first MRM transition and a second intensity is measured for the second MRM transition. A ratio of the first intensity to the second intensity is calculated. A theoretical ratio of the quantity of first precursor ion to the second precursor ion is calculated according to their isotopic relationship. A difference between the ratio and the theoretical ratio is calculated and compared to a threshold value. If the difference is less than the threshold value, the first intensity of the first MRM transition is identified as including an interference for the compound of interest.

公开(公告)号：US20230005727A1

公开(公告)日：2023-01-05

申请号：US17755597

申请日：2020-09-22

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： Yves Le Blanc

IPC: H01J49/00

Abstract: In a DIA method, a specified precursor ion m/z range of interest is divided into a set of two or more precursor ion mass selection windows. A tandem mass spectrometer is instructed to select, dissociate using a first dissociation technique, and mass analyze each precursor ion mass selection window of the set within a specified cycle time. Product ion intensity and m/z measurements are produced for each window of the set using the first dissociation technique. The tandem mass spectrometer is also instructed to select, dissociate using a second dissociation technique, and mass analyze each precursor ion mass selection window of the set within the same cycle time. Product ion intensity and m/z measurements are produced for each window of the set using the second dissociation technique. Product ion measurements from both the first and second dissociation techniques are used to identify or quantitate compounds of a sample.

公开(公告)号：US20220359181A1

公开(公告)日：2022-11-10

申请号：US17753035

申请日：2020-08-22

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： Yves Le Blanc ,  David M. Cox

IPC: H01J49/04 ,  H01J49/00 ,  H01J49/16

Abstract: Before a sample is introduced into a liquid sample delivery device, an ion source device receives aqueous mobile phase solution from the liquid sample delivery device and ionizes compounds of the aqueous mobile phase solution, producing an ion beam. A tandem mass spectrometer performs a first neutral loss scan of the ion beam with a first neutral loss value set to a molecular weight of a first known solvent, producing a first intensity, and performs a second neutral loss scan of the ion beam with a second neutral loss value set to a molecular weight of a second known solvent, producing a second intensity. A ratio of the first intensity to the second intensity is calculated. It is determined if the aqueous mobile phase solution is properly being delivered by the liquid sample delivery device based on the ratio.

公开(公告)号：US11002744B2

公开(公告)日：2021-05-11

申请号：US16626732

申请日：2018-06-28

Applicant: DH Technologies Development Pte. Ltd.

Inventor： Takashi Baba ,  Yves Le Blanc

IPC: G01N33/68 ,  H01J49/00 ,  H01J49/02 ,  H01J49/42

Abstract: A system and method is described for characterizing glycopeptides which includes a first quadrupole mass filter, a multipole rod set of an ion guide, a lens electrode, an ExD device and a mass analyzer. The multipole rod set is adapted to receive a radial radio frequency (RF) trapping voltage and a radial dipole direct current (DC) voltage The lens electrode is adapted to receive an axial trapping alternating current (AC) voltage and a DC voltage. The ExD device performs electron capture dissociation or electron transfer dissociation, the ExD device being positioned so that an entrance of the ExD device is disposed on the other side of the lens electrode opposite the multipole rod set. The mass analyzer is positioned at an exit of the ExD device for receiving ions from the ExD device.

公开(公告)号：US20200279725A1

公开(公告)日：2020-09-03

申请号：US16646674

申请日：2018-09-25

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： Yves Le Blanc

IPC: H01J49/00 ,  H01J49/42

Abstract: An interference in a first MRM transition measurement for a compound of interest is determined by using a second MRM transition that includes an isotope of the precursor ion in the first MRM transition. Both transitions include the same product ion. A first intensity is measured for the first MRM transition and a second intensity is measured for the second MRM transition. A ratio of the first intensity to the second intensity is calculated. A theoretical ratio of the quantity of first precursor ion to the second precursor ion is calculated according to their isotopic relationship. A difference between the ratio and the theoretical ratio is calculated and compared to a threshold value. If the difference is less than the threshold value, the first intensity of the first MRM transition is identified as including an interference for the compound of interest.

公开(公告)号：US20170248548A1

公开(公告)日：2017-08-31

申请号：US15516387

申请日：2015-09-21

Applicant: DH Technologies Development Pte. Ltd.

Inventor： John Lawrence Campbell ,  Eva Duchoslav ,  Yves Le Blanc ,  David M. Cox

IPC: G01N27/62

CPC classification number: G01N27/624 ,  H01J49/0031 ,  H01J49/0036 ,  H01J49/004 ,  H01J49/0045

Abstract: Systems and methods are provided for providing a DMS precursor ion survey scan. An ion source configured to receive a sample is instructed to ionize the sample using a processor. A DMS device configured to receive ions from the ion source is instructed to separate precursor ions received from the ion source and transmit precursor ions using two or more CoVs using the processor. A mass analyzer configured to receive transmitted precursor ions from the DMS device is instructed to measure the m/z intensities of the transmitted precursor ions across an m/z range at each CoV of the two or more CoVs using the processor. The measured m/z intensities of the transmitted precursor ions received from the mass analyzer are stored as a function of m/z value and CoV using the processor. This produces a stored two-dimensional mapping of m/z intensities of the precursor ions of the sample.

公开(公告)号：US20140357502A1

公开(公告)日：2014-12-04

申请号：US14365983

申请日：2012-11-28

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD

Inventor： John Lawrence Campbell ,  Yves Le Blanc ,  Alexandre V. Loboda ,  Igor Chernushevich

IPC: G06F19/00

CPC classification number: G06F19/703 ,  H01J49/0031 ,  H01J49/004

Abstract: In a first location of a mass spectrometer, a plurality of ionized molecules of an ion source are selected that have mass-to-charge ratios within a mass-to-charge ratio window width. The plurality of selected ionized molecules are transmitted from a first to a second location. Reagent ions are transmitted to the second location to reduce a charge state of one or more of the plurality of selected ionized molecules. A mass analyzer is used to analyze the plurality of reduced ionized molecules and produce a mass spectrum. A compound is identified from a peak of the spectrum that has a mass-to-charge ratio less than or equal to the highest mass-to-charge ratio in the window width if the noise is multiply charged and greater than the highest mass-to-charge ratio in the window width if the noise is singly charged.

Abstract translation: 在质谱仪的第一位置，选择离子源的多个离子化分子，其具有在质荷比窗口宽度之内的质荷比。 多个选择的电离分子从第一位置传递到第二位置。 试剂离子被传递到第二位置以减少多个选定的电离分子中的一个或多个的电荷状态。 质量分析器用于分析多个还原离子化分子并产生质谱。 如果噪声是多次充电并且大于最高质量 - 到达的质量 - 荷电比，如果光谱的峰值具有小于或等于窗口宽度中的最高质荷比的化合物， 如果噪声被单独充电，则窗口宽度的充电比例。

公开(公告)号：US12112934B2

公开(公告)日：2024-10-08

申请号：US17753036

申请日：2020-08-22

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： Yves Le Blanc

IPC: H01J49/00 ,  H01J49/10

CPC classification number: H01J49/0036 ,  H01J49/0045 ,  H01J49/10

Abstract: Before each sample of a series of batch samples is introduced into a liquid sample delivery device, an ion source device receives aqueous mobile phase solution from the liquid sample delivery device and ionizes its compounds, producing an ion beam. A tandem mass spectrometer performs a neutral loss or precursor ion scan on the ion beam to measure intensities of two or more precursor ions corresponding to a known aqueous mobile phase solution compound. Intensity measurements for each of the two or more different precursor ions are compared to previously stored intensities to determine the threshold times at which these measurements indicate orifice contamination. A threshold time is then predicted for a known compound of interest of the batch samples based on the m/z value of the known compound of interest and the m/z value and the threshold time of each of the two or more different precursor ions.

公开(公告)号：US11761926B2

公开(公告)日：2023-09-19

申请号：US17814258

申请日：2022-07-22

Applicant: DH TECHNOLOGIES DEVELOPMENT PTE. LTD.

Inventor： Yves Le Blanc ,  Brendon Seale

IPC: G01N27/624 ,  H01J49/00 ,  H01J49/40 ,  H01J49/42

CPC classification number: G01N27/624 ,  H01J49/0031 ,  H01J49/0045 ,  H01J49/40 ,  H01J49/4215

Abstract: In DM-SWATH a plurality of CoVs and a precursor ion mass range are received. A processor performs an iterative series of steps for each CoV of the plurality of CoVs. For each CoV of the plurality of CoVs, the CoV is applied to the DMS device to select a group of precursor ions. A mass filter is instructed to select precursor ions of the group that are within the precursor ion mass range, producing a subgroup of precursor ions. A fragmentation device is instructed to fragment the subgroup of precursor ions, producing a group of product ions. A mass analyzer is instructed to measure the intensity and m/z of the group of product ions, producing a product ion spectrum for each CoV of the plurality of CoVs. DM-SWATH is further used to validate if a known compound is in a sample.