HAPLOID MAIZE TRANSFORMATION
    4.
    发明申请
    HAPLOID MAIZE TRANSFORMATION 审中-公开
    HAPLOID MAIZE转换

    公开(公告)号:US20150307889A1

    公开(公告)日:2015-10-29

    申请号:US14695434

    申请日:2015-04-24

    CPC classification number: C12N15/8213

    Abstract: Disclosed are methods for transformation of an androgenic-derived, haploid cell line with a site-specific nuclease. In some embodiments, the androgenic-derived, haploid cell line is a maize microspore-derived plant tissue culture. In addition, the disclosure provides a method for modifying, e.g., by mutating or targeting and integrating donor DNA into, a specific locus of a haploid or dihaploid tissue genome. The disclosure further provides methods for regenerating a whole plant from the haploid or dihaploid tissue that contains either the mutation at a specific genomic locus or a donor DNA integrated within a specific genomic locus may be obtained from the subject disclosure.

    Abstract translation: 公开了用位点特异性核酸酶转化雄激素衍生的单倍体细胞系的方法。 在一些实施方案中,雄激素衍生的单倍体细胞系是来自玉米小孢子的植物组织培养物。 此外,本公开提供了修饰,例如通过突变或靶向并将供体DNA整合到单倍体或双倍体组织基因组的特定基因座中的方法。 本公开进一步提供了从单倍体或双倍体组织再生整个植物的方法,所述单体或双倍体组织含有特定基因组座位上的突变或整合在特定基因组基因组中的供体DNA,可从本发明公开内容获得。

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