公开(公告)号：US20180142249A1

公开(公告)日：2018-05-24

申请号：US15797285

申请日：2017-10-30

Applicant: Dow AgroSciences LLC

Inventor： Sandeep Kumar ,  Joseph F. Petolino ,  Andrew F. Worden ,  Pierluigi Barone ,  Matthew A. Simpson ,  Tonya L. Strange

IPC: C12N15/82

CPC classification number: C12N15/8213 ,  C07K2319/81 ,  C12N15/8201 ,  C12N15/8209 ,  C12N15/8222 ,  C12N15/8231 ,  C12N15/8234 ,  C12N15/8241 ,  C12N15/8261 ,  C12N15/8274 ,  C12N15/8286

Abstract: Compositions and methods to modify at least one target locus in a plant cell are provided, which comprises providing a plant cell, a plant, or a plant part with one or more target loci and one or more donor loci, providing at least one cleaving site specific nuclease to produce a double strand break within the target loci, followed by non-homologous end joining of at least one donor locus within at least one target locus. Target loci, donor loci and nuclease loci used in these methods, and plant cells, plants and plant parts comprising these target loci, donor loci, nuclease loci and/or the recombined loci are also provided.

公开(公告)号：US20170283865A1

公开(公告)日：2017-10-05

申请号：US15249684

申请日：2016-08-29

Applicant: Dow AgroSciences LLC

Inventor： Lakshmi Sastry-Dent ,  Matthew A. Simpson ,  Zehui Cao ,  Wei Chen ,  Ning Zhou ,  Steven R. Webb

IPC: C12Q1/68 ,  A01G31/00 ,  C12N15/70 ,  C12N15/66 ,  C40B40/08 ,  C12N15/10

CPC classification number: C12Q1/6858 ,  A01G24/00 ,  C12N5/00 ,  C12N15/1082 ,  C12N15/66 ,  C12N15/70 ,  C12Q1/6895 ,  C12Q2600/13 ,  C12Q2600/156 ,  C40B40/08

Abstract: The present disclosure provides methods for detecting and identifying plant events that contain precision targeted genomic loci, and plants and plant cells comprising such targeted genomic loci. The method can be deployed as a high throughput process utilized for screening the intactness or disruption of a targeted genomic loci and optionally for detecting a donor DNA polynucleotide insertion at the targeted genomic loci. The methods are readily applicable for the identification of plant events produced via a targeting method which results from the use of a site specific nuclease.

公开(公告)号：US10344322B2

公开(公告)日：2019-07-09

申请号：US15249684

申请日：2016-08-29

Applicant: Dow AgroSciences LLC

Inventor： Lakshmi Sastry-Dent ,  Matthew A. Simpson ,  Zehui Cao ,  Wei Chen ,  Ning Zhou ,  Steven R. Webb

IPC: C12Q1/68 ,  C12Q1/6858 ,  C12Q1/6895 ,  C12N15/10 ,  C12N15/66 ,  C12N15/70 ,  C40B40/08 ,  A01G24/00 ,  C12N5/00

Abstract: The present disclosure provides methods for detecting and identifying plant events that contain precision targeted genomic loci, and plants and plant cells comprising such targeted genomic loci. The method can be deployed as a high throughput process utilized for screening the intactness or disruption of a targeted genomic loci and optionally for detecting a donor DNA polynucleotide insertion at the targeted genomic loci. The methods are readily applicable for the identification of plant events produced via a targeting method which results from the use of a site specific nuclease.

公开(公告)号：US20150307889A1

公开(公告)日：2015-10-29

申请号：US14695434

申请日：2015-04-24

Applicant: Dow AgroSciences LLC

Inventor： Joseph F. Petolino ,  Tonya L. Strange ,  Jayakumar Pon Samuel ,  Ryan C. Blue ,  Matthew A. Simpson

IPC: C12N15/82

CPC classification number: C12N15/8213

Abstract: Disclosed are methods for transformation of an androgenic-derived, haploid cell line with a site-specific nuclease. In some embodiments, the androgenic-derived, haploid cell line is a maize microspore-derived plant tissue culture. In addition, the disclosure provides a method for modifying, e.g., by mutating or targeting and integrating donor DNA into, a specific locus of a haploid or dihaploid tissue genome. The disclosure further provides methods for regenerating a whole plant from the haploid or dihaploid tissue that contains either the mutation at a specific genomic locus or a donor DNA integrated within a specific genomic locus may be obtained from the subject disclosure.

Abstract translation: 公开了用位点特异性核酸酶转化雄激素衍生的单倍体细胞系的方法。 在一些实施方案中，雄激素衍生的单倍体细胞系是来自玉米小孢子的植物组织培养物。 此外，本公开提供了修饰，例如通过突变或靶向并将供体DNA整合到单倍体或双倍体组织基因组的特定基因座中的方法。 本公开进一步提供了从单倍体或双倍体组织再生整个植物的方法，所述单体或双倍体组织含有特定基因组座位上的突变或整合在特定基因组基因组中的供体DNA，可从本发明公开内容获得。