公开(公告)号：US20170327912A1

公开(公告)日：2017-11-16

申请号：US15589039

申请日：2017-05-08

Applicant: DSM IP ASSETS B.V.

Inventor： Roelof Hendrik Matthijs Kouwen ,  Douwe Van Sinderen ,  Brian McDonnell

IPC: C12Q1/70

CPC classification number: C12Q1/701 ,  C12Q1/70 ,  C12Q2600/158 ,  C12Q2600/16

Abstract: The present invention relates to a kit suitable for detection of Streptococcus thermophilus infecting phages, said kit comprising: (a) a first primer pair suitable to generate an amplicon characteristic for a 987 phage; and (b) a second primer pair suitable to generate an amplicon characteristic for a 5093 phage. Further, the present invention relates to a method for detecting the presence of Streptococcus thermophilus infecting phages in a sample.

公开(公告)号：US10244771B2

公开(公告)日：2019-04-02

申请号：US15327661

申请日：2015-07-23

Applicant: DSM IP ASSETS B.V.

Inventor： Roelof Hendrik Matthijs Kouwen ,  Laurens Leendert Hanemaaijer ,  Douwe Van Sinderen ,  Brian McDonnell ,  Jennifer Mahony

IPC: A23C9/123 ,  C12N15/90 ,  C12N15/74 ,  C12N15/113 ,  C12N1/20 ,  A23C19/032 ,  C12N1/36

Abstract: The present invention provides a method for the construction of a bacteriophage-insensitive mutant of a Streptococcus thermophilus parent strain whereby the bacteriophage insensitivity is not based on the CRISPR resistance mechanism but based on another mechanism. The method provides a protocol by which this can be achieved by inactivating one or more of the CRISPR systems present in a given strain. The invention also provides the bacteriophage-insensitive mutants as well as their use in a process for making a dairy product.

公开(公告)号：US10519519B2

公开(公告)日：2019-12-31

申请号：US16025700

申请日：2018-07-02

Applicant: DSM IP ASSETS B.V.

Inventor： Roelof Hendrik Matthijs Kouwen ,  Pim Van Hee ,  Douwe Van Sinderen ,  Brian McDonnell ,  Jennifer Mahony

IPC: A23C9/123 ,  C12N15/01 ,  A23C19/032 ,  C12N1/20 ,  C12R1/46 ,  G16B30/00 ,  C12N1/04 ,  C12Q1/689

Abstract: Bacteriophage Insensitive Mutants (BIMs) of three Streptococcus thermophilus parent strains were generated and characterized for phage sensitivity, sedimentation rate, cell chain length, phage adsorption and CRISPR loci alterations. Several BIMs showed an altered sedimentation phenotype as well as an increase cell chain length, reduced phage sensitivity, reduced phage adsorption and 100% identity in three CRISPR loci. The results show that the derived BIMs have become phage-resistant through a mechanism other than CRISPR.

公开(公告)号：US20180305777A1

公开(公告)日：2018-10-25

申请号：US16025700

申请日：2018-07-02

Applicant: DSM IP ASSETS B.V.

Inventor： Roelof Hendrik Matthijs KOUWEN ,  Pim Van Hee ,  Douwe Van Sinderen ,  Brian McDonnell ,  Jennifer Mahony

IPC: C12R1/46 ,  G06F19/22 ,  C12N1/20 ,  C12N1/04 ,  C12N15/01 ,  C12Q1/689 ,  A23C9/123 ,  A23C19/032

Abstract: Bacteriophage Insensitive Mutants (BIMs) of three Streptococcus thermophilus parent strains were generated and characterized for phage sensitivity, sedimentation rate, cell chain length, phage adsorption and CRISPR loci alterations. Several BIMs showed an altered sedimentation phenotype as well as an increase cell chain length, reduced phage sensitivity, reduced phage adsorption and 100% identity in three CRISPR loci. The results show that the derived BIMs have become phage-resistant through a mechanism other than CRISPR.

公开(公告)号：US11674117B2

公开(公告)日：2023-06-13

申请号：US16470856

申请日：2017-12-14

Applicant: DSM IP Assets B.V.

Inventor： Roelof Hendrik Matthijs Kouwen ,  Douwe Van Sinderen ,  Brian McDonnell ,  Pieter Emiel Verloren Van Themaat ,  Jennifer Mahony

IPC: C12N1/20 ,  A23C9/123 ,  C12Q1/689 ,  C12R1/46 ,  G16B35/20

CPC classification number: C12N1/20 ,  A23C9/1238 ,  C12N1/205 ,  C12Q1/689 ,  G16B35/20 ,  A23Y2240/75 ,  C12R2001/46

Abstract: The present invention relates to a starter culture comprising at least two Streptococcus thermophilus strains, wherein a first and a second Streptococcus thermophilus strain are chosen from RGP group 1, RGP group 2, RGP group 3 and RGP group 4, with the proviso that the first and second Streptococcus thermophilus strains do not belong to the same RGP group.

公开(公告)号：US10519516B2

公开(公告)日：2019-12-31

申请号：US15589039

申请日：2017-05-08

Applicant: DSM IP ASSETS B.V.

Inventor： Roelof Hendrik Matthijs Kouwen ,  Douwe Van Sinderen ,  Brian McDonnell

IPC: C12Q1/70 ,  C12N15/00

Abstract: The present invention relates to a kit suitable for detection of Streptococcus thermophilus infecting phages, said kit comprising: (a) a first primer pair suitable to generate an amplicon characteristic for a 987 phage; and (b) a second primer pair suitable to generate an amplicon characteristic for a 5093 phage. Further, the present invention relates to a method for detecting the presence of Streptococcus thermophilus infecting phages in a sample.

公开(公告)号：US10041135B2

公开(公告)日：2018-08-07

申请号：US15119708

申请日：2015-02-20

Applicant: DSM IP ASSETS B.V.

Inventor： Roelof Hendrik Matthijs Kouwen ,  Pim Van Hee ,  Douwe Van Sinderen ,  Brian McDonnell ,  Jennifer Mahony

IPC: C12N1/20 ,  C12N1/04 ,  G06F19/22 ,  A23C9/123 ,  A23C19/032 ,  C12R1/46

Abstract: Bacteriophage Insensitive Mutants (BIMs) of three Streptococcus thermophilus parent strains were generated and characterized for phage sensitivity, sedimentation rate, cell chain length, phage adsorption and CRISPR loci alterations. Several BIMs showed an altered sedimentation phenotype as well as an increase cell chain length, reduced phage sensitivity, reduced phage adsorption and 100% identity in three CRISPR loci. The results show that the derived BIMs have become phage-resistant through a mechanism other than CRISPR.