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    POLYPEPTIDES HAVING CELLULOLYTIC ENHANCING ACTIVITY AND USES THEREOF
    3.
    发明申请
    POLYPEPTIDES HAVING CELLULOLYTIC ENHANCING ACTIVITY AND USES THEREOF 有权

    公开(公告)号：US20210123030A1

    公开(公告)日：2021-04-29

    申请号：US17137514

    申请日：2020-12-30

    Applicant: DSM IP ASSETS B.V.

    Inventor： Johannes Gustaaf Ernst VAN LEEUWEN ,  Loes Elizabeth BEVERS

    IPC: C12N9/02 ,  C12N9/42 ,  C12P7/10 ,  C12P19/14 ,  C12N15/52 ,  C12N1/14 ,  C12N15/80

    Abstract: The invention relates to a polypeptide having cellulolytic enhancing activity, wherein the polypeptide is selected from the group consisting of: (a) a polypeptide comprising an amino acid sequence having at least 60% sequence identity with the amino acid sequence of SEQ ID NO: 2, 5, 8, 11, 14, 17, 20, 23, 26, 29, 32, 35, 38, 41, 44 and/or 47; (b) a polypeptide encoded by a polynucleotide comprising a nucleotide sequence having at least 60% sequence identity to the nucleotide sequence of SEQ ID NO: 3, 6, 9, 12, 15, 18, 21, 24, 27, 30, 33, 36, 39, 42, 45 and/or 48; (c) a polypeptide encoded by a polynucleotide comprising a nucleotide sequence which hybridises under at least high stringency conditions with the complementary strand of SEQ ID NO: 3, 6, 9, 12, 15, 18, 21, 24, 27, 30, 33, 36, 39, 42, 45 and/or 48; and (d) a fragment of the polypeptide of (a), (b), or (c), that has cellulolytic enhancing activity.

    PROCESS FOR ENZYMATIC HYDROLYSIS OF LIGNOCELLULOSIC MATERIAL AND FERMENTATION OF SUGARS
    8.
    发明申请
    PROCESS FOR ENZYMATIC HYDROLYSIS OF LIGNOCELLULOSIC MATERIAL AND FERMENTATION OF SUGARS 有权

    公开(公告)号：US20210180098A1

    公开(公告)日：2021-06-17

    申请号：US17170169

    申请日：2021-02-08

    Applicant: DSM IP ASSETS B.V.

    Inventor： Bertus NOORDAM ,  Loes Elizabeth BEVERS ,  Rudy Francois MariaJozef PARTON ,  Michael Petrus Jozef BERKHOUT

    IPC: C12P19/02 ,  C13K1/02 ,  D21C3/26 ,  C13K13/00 ,  C12P19/14

    Abstract: The invention relates to a process for the preparation of a fermentation product from lignocellulosic material, comprising the following steps: a) optionally, pretreatment of the lignocellulosic material, b) optionally, washing of the optionally pretreated lignocellulosic material, c) enzymatic hydrolysis of the optionally washed and/or optionally pretreated lignocellulosic material using an enzyme composition comprising at least two cellulases and whereby the enzyme composition at least comprises LPMO, and optionally purifying the hydrolysed lignocellulosic material, d) fermentation of the hydrolysed lignocellulosic material to produce a fermentation product, and e) optionally, recovery of a fermentation product, wherein the amounts of formed hydrolysed oxidation products at the end of the enzymatic hydrolysis by the oxidation by LPMO of the lignocellulosic material containing cellulose and/or cello-oligosaccharides is kept between 3 to 80 g/kg glucan present in the lignocellulosic material by adding a suitable amount of oxygen after the pre-treatment and before and/or during the enzymatic hydrolysis to the lignocellulosic material, preferably the formed hydrolysed oxidation product is gluconic acid, an aldonic acid and/or geminal diol, more preferably the hydrolysed oxidation product is gluconic acid.

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