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    PROCESS FOR ENZYMATIC HYDROLYSIS OF LIGNOCELLULOSIC MATERIAL
    2.
    发明申请
    PROCESS FOR ENZYMATIC HYDROLYSIS OF LIGNOCELLULOSIC MATERIAL 审中-公开
    木质纤维素材料的酶解水解方法

    公开(公告)号：US20150322471A1

    公开(公告)日：2015-11-12

    申请号：US14655349

    申请日：2014-01-09

    申请人： DSM IP ASSETS B.V.

    发明人： Johannes Petrus SMITS ,  Michael Petrus Jozef BERKHOUT ,  Bertus NOORDAM

    IPC分类号： C12P19/14 ,  C12P19/02

    CPC分类号： C12P19/02 ,  C12P19/14 ,  C12P2201/00 ,  C12Y302/01004 ,  C13K1/02

    摘要： The invention relates to a process for the hydrolysis of cellulose containing biomass which comprises a liquefaction step in which a first enzyme or first enzyme composition is added to liquefy at least part of the solids present in the biomass and to keep the viscosity of the cellulose containing biomass below 1000 cP, preferably below 800 cP, more preferably below 600 cP in the liquefaction step; followed by a saccharification step in which a second enzyme composition is added to form oligomeric and/or monomeric sugars; and whereby the first enzyme or first enzyme composition is different from the second enzyme composition; whereby the first enzyme or first enzyme composition comprises an endoglucanase; whereby the second enzyme composition comprises a cellulase; and whereby the first enzyme or first enzyme composition comprises more endogluconase than the second enzyme composition (expressed in protein wt %).

    摘要翻译： 本发明涉及一种水解含纤维素生物质的方法，其包括液化步骤，其中加入第一种酶或第一种酶组合物来液化存在于生物质中的至少部分固体并保持含有纤维素的粘度 生物质在液化步骤中低于1000cP，优选低于800cP，更优选低于600cP; 随后是糖化步骤，其中加入第二种酶组合物以形成低聚和/或单体糖; 并且由此所述第一酶或第一酶组合物不同于所述第二酶组合物; 由此第一酶或第一酶组合物包含内切葡聚糖酶; 由此第二酶组合物包含纤维素酶; 并且由此第一酶或第一酶组合物包含比第二酶组合物更多的内切葡聚糖酶（以蛋白质wt％表示）。

    PROCESS FOR ENZYMATIC HYDROLYSIS OF LIGNOCELLULOSIC MATERIAL AND FERMENTATION OF SUGARS
    3.
    发明申请
    PROCESS FOR ENZYMATIC HYDROLYSIS OF LIGNOCELLULOSIC MATERIAL AND FERMENTATION OF SUGARS 有权

    公开(公告)号：US20210180098A1

    公开(公告)日：2021-06-17

    申请号：US17170169

    申请日：2021-02-08

    申请人： DSM IP ASSETS B.V.

    发明人： Bertus NOORDAM ,  Loes Elizabeth BEVERS ,  Rudy Francois MariaJozef PARTON ,  Michael Petrus Jozef BERKHOUT

    IPC分类号： C12P19/02 ,  C13K1/02 ,  D21C3/26 ,  C13K13/00 ,  C12P19/14

    摘要： The invention relates to a process for the preparation of a fermentation product from lignocellulosic material, comprising the following steps: a) optionally, pretreatment of the lignocellulosic material, b) optionally, washing of the optionally pretreated lignocellulosic material, c) enzymatic hydrolysis of the optionally washed and/or optionally pretreated lignocellulosic material using an enzyme composition comprising at least two cellulases and whereby the enzyme composition at least comprises LPMO, and optionally purifying the hydrolysed lignocellulosic material, d) fermentation of the hydrolysed lignocellulosic material to produce a fermentation product, and e) optionally, recovery of a fermentation product, wherein the amounts of formed hydrolysed oxidation products at the end of the enzymatic hydrolysis by the oxidation by LPMO of the lignocellulosic material containing cellulose and/or cello-oligosaccharides is kept between 3 to 80 g/kg glucan present in the lignocellulosic material by adding a suitable amount of oxygen after the pre-treatment and before and/or during the enzymatic hydrolysis to the lignocellulosic material, preferably the formed hydrolysed oxidation product is gluconic acid, an aldonic acid and/or geminal diol, more preferably the hydrolysed oxidation product is gluconic acid.

    PROCESS FOR ENZYMATIC HYDROLYSIS OF LIGNOCELLULOSIC MATERIAL AND FERMENTATION OF SUGARS
    6.
    发明申请
    PROCESS FOR ENZYMATIC HYDROLYSIS OF LIGNOCELLULOSIC MATERIAL AND FERMENTATION OF SUGARS 有权

    公开(公告)号：US20210115482A1

    公开(公告)日：2021-04-22

    申请号：US17115429

    申请日：2020-12-08

    申请人： DSM IP ASSETS B.V.

    发明人： Bertus NOORDAM ,  Michael Petrus Jozef BERKHOUT

    IPC分类号： C12P19/02 ,  C13K1/02 ,  D21C3/26 ,  C13K13/00 ,  C12P19/14

    摘要： The invention relates to a process for the preparation of a fermentation product from lignocellulosic material, comprising the following steps: a) optionally, pre-treatment of the lignocellulosic material, b) optionally, washing of the optionally pretreated lignocellulosic material, c) enzymatic hydrolysis of the optionally washed and/or optionally pretreated lignocellulosic material using an enzyme composition comprising at least two cellulases and whereby the enzyme composition at least comprises LPMO, and optionally purifying the hydrolysed lignocellulosic material, d) fermentation of the hydrolysed lignocellulosic material to produce a fermentation product, and e) optionally, recovery of a fermentation product, wherein oxygen is consumed in amounts corresponding to between 20 and 5000 mmol molecular oxygen per kg glucan present in the lignocellulosic material, the oxygen is added after the pretreatment and before and/or during the enzymatic hydrolysis of the lignocellulosic material, preferably in an amount corresponding to at least 30 mmol molecular oxygen per kg glucan present in the lignocellulosic material, more preferably in an amount corresponding to at least 40 mmol molecular oxygen per kg glucan present in the lignocellulosic material, and most preferably in an amount corresponding to at least 50 mmol molecular oxygen per kg glucan present in the lignocellulosic material is consumed.

    PROCESS FOR ENZYMATIC HYDROLYSIS OF LIGNOCELLULOSIC MATERIAL AND FERMENTATION OF SUGARS
    8.
    发明申请
    PROCESS FOR ENZYMATIC HYDROLYSIS OF LIGNOCELLULOSIC MATERIAL AND FERMENTATION OF SUGARS 审中-公开

    公开(公告)号：US20200048669A1

    公开(公告)日：2020-02-13

    申请号：US16528191

    申请日：2019-07-31

    申请人： DSM IP ASSETS B.V.

    发明人： Bertus NOORDAM ,  Michael Petrus Jozef BERKHOUT

    IPC分类号： C12P19/02 ,  C13K13/00 ,  D21C3/26 ,  C13K1/02 ,  C12P19/14

    摘要： The invention relates to a process for the preparation of a fermentation product from lignocellulosic material, comprising the following steps: a) optionally, pre-treatment of the lignocellulosic material, b) optionally, washing of the optionally pretreated lignocellulosic material, c) enzymatic hydrolysis of the optionally washed and/or optionally pretreated lignocellulosic material using an enzyme composition comprising at least two cellulases and whereby the enzyme composition at least comprises LPMO, and optionally purifying the hydrolysed lignocellulosic material, d) fermentation of the hydrolysed lignocellulosic material to produce a fermentation product, and e) optionally, recovery of a fermentation product, wherein oxygen is consumed in amounts corresponding to between 20 and 5000 mmol molecular oxygen per kg glucan present in the lignocellulosic material, the oxygen is added after the pretreatment and before and/or during the enzymatic hydrolysis of the lignocellulosic material, preferably in an amount corresponding to at least 30 mmol molecular oxygen per kg glucan present in the lignocellulosic material, more preferably in an amount corresponding to at least 40 mmol molecular oxygen per kg glucan present in the lignocellulosic material, and most preferably in an amount corresponding to at least 50 mmol molecular oxygen per kg glucan present in the lignocellulosic material is consumed.

    PROCESS FOR ENZYMATIC HYDROLYSIS OF LIGNOCELLULOSIC MATERIAL AND FERMENTATION OF SUGARS
    9.
    发明申请
    PROCESS FOR ENZYMATIC HYDROLYSIS OF LIGNOCELLULOSIC MATERIAL AND FERMENTATION OF SUGARS 审中-公开
    木质纤维素材料的酶解水解和糖的发酵工艺

    公开(公告)号：US20170044584A1

    公开(公告)日：2017-02-16

    申请号：US15307046

    申请日：2015-04-29

    申请人： DSM IP ASSETS B.V.

    发明人： Bertus NOORDAM ,  Michael Petrus Jozef BERKHOUT

    IPC分类号： C12P19/14 ,  C12P19/02

    CPC分类号： C12P19/02 ,  C12P19/14 ,  C12P2203/00 ,  C13K1/02 ,  C13K13/00 ,  C13K13/002 ,  D21C3/26 ,  Y02E50/16

    摘要： The invention relates to a process for the preparation of a fermentation product from lignocellulosic material, comprising the following steps: a) optionally, pre-treatment of the lignocellulosic material, b) optionally, washing of the optionally pretreated lignocellulosic material, c) enzymatic hydrolysis of the optionally washed and/or optionally pretreated lignocellulosic material using an enzyme composition comprising at least two cellulases and whereby the enzyme composition at least comprises LPMO, and optionally purifying the hydrolysed lignocellulosic material, d) fermentation of the hydrolysed lignocellulosic material to produce a fermentation product, and e) optionally, recovery of a fermentation product, wherein oxygen is consumed in amounts corresponding to between 20 and 5000 mmol molecular oxygen per kg glucan present in the lignocellulosic material, the oxygen is added after the pretreatment and before and/or during the enzymatic hydrolysis of the lignocellulosic material, preferably in an amount corresponding to at least 30 mmol molecular oxygen per kg glucan present in the lignocellulosic material, more preferably in an amount corresponding to at least 40 mmol molecular oxygen per kg glucan present in the lignocellulosic material, and most preferably in an amount corresponding to at least 50 mmol molecular oxygen per kg glucan present in the lignocellulosic material is consumed.

    摘要翻译： 本发明涉及从木质纤维素材料制备发酵产物的方法，包括以下步骤：a）任选地，木质纤维素材料的预处理，b）任选地，任选地，洗涤任选预处理的木质纤维素材料，c）酶水解 任选地使用包含至少两种纤维素酶的酶组合物洗涤和/或任选地预处理的木质纤维素材料，并且由此所述酶组合物至少包含LPMO，并任选地纯化水解的木质纤维素材料，d）水解的木质纤维素材料的发酵以产生发酵产物， 和e）任选地，回收发酵产物，其中在预处理之后和在酶水解之前和/或期间，向木质纤维素材料中加入电子给体和氧。

    PROCESS FOR ENZYMATIC HYDROLYSIS OF LIGNOCELLULOSIC MATERIAL AND FERMENTATION OF SUGARS
    10.
    发明申请
    PROCESS FOR ENZYMATIC HYDROLYSIS OF LIGNOCELLULOSIC MATERIAL AND FERMENTATION OF SUGARS 有权
    木质纤维素材料的酶解水解和糖的发酵工艺

    公开(公告)号：US20150307903A1

    公开(公告)日：2015-10-29

    申请号：US14440662

    申请日：2013-11-07

    申请人： DSM IP ASSETS B.V.

    发明人： Bertus NOORDAM ,  Michael Petrus Jozef BERKHOUT

    IPC分类号： C12P7/10 ,  C12P19/14 ,  C12P7/56 ,  C12P7/42 ,  C12P7/40 ,  C12P7/54 ,  C12P7/46 ,  C12P7/48 ,  C12P7/18 ,  C12P5/02 ,  C12P7/20 ,  C12P7/16 ,  C12P17/10 ,  C12P35/00 ,  C12P19/02

    CPC分类号： C12P7/10 ,  C12P5/026 ,  C12P7/16 ,  C12P7/18 ,  C12P7/20 ,  C12P7/40 ,  C12P7/42 ,  C12P7/46 ,  C12P7/48 ,  C12P7/54 ,  C12P7/56 ,  C12P17/10 ,  C12P19/02 ,  C12P19/14 ,  C12P35/00 ,  C12P2203/00 ,  Y02E50/16 ,  Y02E50/343

    摘要： The invention relates to a process for the preparation of a fermentation product from ligno-cellulosic material, comprising the following steps: a) optionally pre-treatment of the ligno-cellulosic material; b) optionally washing of the optionally pre-treated ligno-cellulosic material; c) enzymatic hydrolysis of the optionally washed and/or optionally pre-treated ligno-cellulosic material using an enzyme composition comprising at least two cellulase and whereby the enzyme composition at least comprises GH61; d) whereby less than 7.5 mg enzyme composition/g glucan (on dry matter and enzyme as protein) or less than 3.0 mg enzyme composition/g feedstock (on dry matter and enzyme as protein) is used; and e) fermentation of the hydrolysed ligno-cellulosic material to produce a fermentation product; and f) optionally recovery of a fermentation product; wherein before and/or during the enzymatic hydrolysis oxygen is added to the ligno-cellulosic material.

    摘要翻译： 本发明涉及一种从木质纤维素材料制备发酵产物的方法，包括以下步骤：a）任选地预处理木素纤维素材料; b）任选地洗涤任选预处理的木质纤维素材料; c）使用包含至少两种纤维素酶的酶组合物对任选洗涤和/或任选预处理的木质纤维素材料进行酶水解，由此所述酶组合物至少包含GH61; d）使用小于7.5mg酶组合物/ g葡聚糖（以干物质和酶为蛋白质）或小于3.0mg酶组合物/ g原料（以干物质和酶为蛋白质）; 和e）水解的木质纤维素材料的发酵以产生发酵产物; 和f）任选地回收发酵产物; 其中在酶水解之前和/或期间，向木质纤维素材料中加入氧气。