公开(公告)号：US20170306377A1

公开(公告)日：2017-10-26

申请号：US15516854

申请日：2015-10-08

申请人： DSM IP ASSETS B.V.

发明人： Marco Alexander VAN DEN BERG ,  Marcella Katharina MADERN ,  Remko Tsjibbe WINTER

IPC分类号： C12P19/56 ,  C12N15/52 ,  A23L27/30

CPC分类号： C12P19/56 ,  A23L27/33 ,  A23L27/36 ,  A23V2002/00 ,  C12N15/52 ,  C12P19/18 ,  C12Y106/02004 ,  C12Y114/13078 ,  C12Y114/13079 ,  C12Y204/00 ,  C12Y402/03019 ,  C12Y505/01013

摘要： The present invention relates to a method for increasing the degree of glycosylation of a composition comprising steviol glycosides, which method comprises:a. contacting said composition comprising steviol glycosides with a recombinant microorganism, a cell free extract derived from such a microorganism or an enzyme preparation derived from either thereof; and b. thereby to increase the degree of glycosylation of the composition comprising steviol glycosides, wherein the recombinant microorganism comprises one or more nucleotide sequence(s) encoding: a polypeptide having ent-copalyl pyrophosphate synthase activity;a polypeptide having ent-Kaurene synthase activity; a polypeptide having ent-Kaurene oxidase activity; a polypeptide having kaurenoic acid 13-hydroxylase activity; and one or more polypeptides having UDP-glucosyltransferase activity whereby expression of the nucleotide sequence(s) confer(s) on the microorganism the ability to produce at least one steviol glycoside. The present invention also relates to a composition comprising steviol glycosides obtainable by such a method.