公开(公告)号：US20180057850A1

公开(公告)日：2018-03-01

申请号：US15560318

申请日：2016-03-23

Applicant: DSM IP ASSETS B.V.

Inventor： Hendrik Jan BOSCH ,  Martinus Julius BEEKWILDER ,  Viktor Marius BOER

IPC: C12P19/56 ,  C12N9/02 ,  C12N9/10 ,  A23L27/30 ,  A23L2/60

CPC classification number: C12P19/56 ,  A23L2/60 ,  A23L27/36 ,  A23V2002/00 ,  C12N9/0042 ,  C12N9/1048 ,  C12N9/1051 ,  C12Y106/02004 ,  C12Y204/01017

Abstract: The present invention relates to polypeptides having UDP-Glycosyltransferase activity derived from Solanum lycopersicum and having the amino acid sequence set out in any of SEQ ID NO: 1 to 4 or an amino acid sequence having at least about 30% sequence identity thereto. The application also relates to recombinant hosts comprising a recombinant nucleic acid sequence encoding said polypeptides and uses thereof prepare glycosylated diterpenes, like steviol glycoside. The host cells might comprise further enzymes of the steviol glycoside biosynthesis pathway.