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公开(公告)号：US12104139B2
公开(公告)日：2024-10-01
申请号：US16563260
申请日：2019-09-06
申请人： DSM IP ASSETS B.V.
IPC分类号： C11B1/02 , C07C51/42 , C07C51/487 , C11B3/00
CPC分类号： C11B1/02 , C07C51/42 , C07C51/487 , C11B1/025 , C11B3/001 , C07C51/487 , C07C57/03 , C07C51/42 , C07C57/03
摘要： Disclosed herein are processes for obtaining a microbial oil comprising one or more polyunsaturated fatty acids (PUFAs) from one or more microbial cells by lysing the cells to form a lysed cell composition, treating the lysed cell composition to form an oil-containing emulsion and then recovering the oil from the oil-containing emulsion. Further disclosed herein is microbial oil comprising one or more PUFAs that is recovered from microbial cells by at least one process described herein.
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公开(公告)号：US11946017B2
公开(公告)日：2024-04-02
申请号：US16317305
申请日：2017-07-12
发明人： Manfred Bärz , Marc Beiser , Georg Borchers , Stephen Robert Cherinko , Mathias Dernedde , Michael Diehl , Xiao Daniel Dong , Jürgen Haberland , Michael Benjamin Johnson , Robert Cody Kertis , Jochen Lebert , Neil Francis Leininger , Kirt Lyvell Matthews, Sr. , Holger Pfeifer , Christian Rabe , Shannon Elizabeth Ethier Resop , Ginger Marie Shank , Vinod Tarwade , David Allen Tinsley , Daniel Verkoeijen
IPC分类号： C11B1/12 , A23K10/12 , A23K20/158 , C11B1/02
CPC分类号： C11B1/12 , A23K10/12 , A23K20/158 , C11B1/025
摘要： The current invention relates to a method of separating polyunsaturated fatty acids containing lipids from a lipids containing biomass.
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公开(公告)号：US11352651B2
公开(公告)日：2022-06-07
申请号：US16473805
申请日：2017-12-20
发明人： Michael Diehl , Xiao Daniel Dong , Annika Hartmann , Martin Heining , Michael Benjamin Johnson , Jochen Lebert , Neil Francis Leininger , Kirt Lyvell Matthews, Sr. , Mark Edward Nejako, II , Holger Pfeifer , Christian Rabe , Shannon Elizabeth Ethier Resop , Ginger Marie Shank , Vinod Tarwade , David Allen Tinsley
IPC分类号： C12P7/64 , C11B1/10 , C12P7/6463
摘要： The invention relates to the isolation of polyunsaturated fatty acids (PUFAs) from a lipids containing biomass. The main characteristics of the method are that a suspension of cells containing PUFAs is obtained and the cells are optionally lysed. The suspension is concentrated and then incubated for at least an hour at a controlled temperature while adding a specific range of base equivalents to biomass and maintaining a moderate pH. The isolation of lipid may be performed in the absence of organic solvents and without the addition of salt.
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公开(公告)号：US10385289B2
公开(公告)日：2019-08-20
申请号：US15875733
申请日：2018-01-19
申请人： DSM IP Assets B.V.
发明人： Stephen Robert Cherinko , Robert Cody Kertis , Kirk E. Apt , Paul Warren Behrens , Jon Milton Hansen , Joseph W. Pfeifer, III , Tracey Lynn Stahl , Ross Zirkle , Nasrin Tabayehnejad , Krishna Raman , Neil Francis Leininger
摘要： The present invention relates to processes for obtaining a lipid from a cell by lysing the cell, contacting the cell with a base and/or salt, and separating the lipid. The present invention is also directed to a lipid prepared by the processes of the present invention. The present invention is also directed to microbial lipids having a particular anisidine value, peroxide value, and/or phosphorus content.
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公开(公告)号：US11725221B2
公开(公告)日：2023-08-15
申请号：US16636940
申请日：2018-08-09
IPC分类号： C12P7/6472 , A23L33/12 , A23D9/04 , B01D21/26 , C11B1/10
CPC分类号： C12P7/6472 , A23D9/04 , A23L33/12 , B01D21/262 , C11B1/10 , A23V2002/00
摘要： This invention relates to a method of using a two-step serial centrifugation process in extracting nutritive oil from a fermentation broth, this novel method prevents oil yield losses while preserving product quality.
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公开(公告)号：US10364207B2
公开(公告)日：2019-07-30
申请号：US15106371
申请日：2014-12-19
申请人： DSM IP ASSETS B.V.
发明人： Mark Barker , Nasrin Tabayehnejad , Ginger Shank , Neil Francis Leininger , Kirt Lyvell Matthews, Sr.
摘要： Disclosed herein are processes for obtaining a microbial oil comprising one or more polyunsaturated fatty acids (PU-FAs) from one or more microbial cells by lysing the cells to form a lysed cell composition and then recovering the oil from the lysed cell composition. Further disclosed herein is microbial oil comprising one or more PUFAs that is recovered from microbial cells by at least one process described herein.
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公开(公告)号：US20180142183A1
公开(公告)日：2018-05-24
申请号：US15875733
申请日：2018-01-19
申请人： DSM IP Assets B.V.
发明人： Stephen Robert CHERINKO , Robert Cody KERTIS , Kirk E. APT , Paul Warren BEHRENS , Jon Milton HANSEN , Joseph W. PFEIFER, III , Tracey Lynn STAHL , Ross ZIRKLE , Nasrin Tabayehnejad , Krishna Raman , Neil Francis Leininger
摘要： The present invention relates to processes for obtaining a lipid from a cell by lysing the cell, contacting the cell with a base and/or salt, and separating the lipid. The present invention is also directed to a lipid prepared by the processes of the present invention. The present invention is also directed to microbial lipids having a particular anisidine value, peroxide value, and/or phosphorus content.
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