公开(公告)号：US11987819B2

公开(公告)日：2024-05-21

申请号：US16300212

申请日：2017-05-11

Applicant: DSM IP Assets B.V.

Inventor： Anne-Cecile V. Bayne ,  Ross E. Zirkle

IPC: A01H5/00 ,  C12N9/10 ,  C12N15/00 ,  C12N15/82 ,  C12P7/6432 ,  C12P7/6472

CPC classification number: C12N9/1029 ,  A01H5/00 ,  C12N15/00 ,  C12N15/8247 ,  C12P7/6432 ,  C12P7/6472 ,  C12Y203/01085

Abstract: The subject disclosure features, in one aspect, a method for producing lipids enriched for EPA, comprising modifying a microalga to increase expression of PFA1, and/or PFA3, and culturing the modified microalga under conditions which allow the expression of PFA1, and/or PFA3, wherein lipids enriched for EPA are produced. Also featured is a recombinant microalga in which PFA1, and/or PFA3, is overexpressed. Such recombinant microalgae have been demonstrated herein to produce very favorable fatty acid lipid profiles (e.g., increased levels of EPA, increased ratio of EPA:DHA, decreased levels of DPA n-6, etc.).