公开(公告)号：US20210222222A1

公开(公告)日：2021-07-22

申请号：US17051610

申请日：2019-05-21

Applicant: DSM IP ASSETS B.V.

Inventor： Christopher Mark FARRELL ,  Lisa Ann LAPRADE ,  Otto Martin LEHMANN ,  Joshua TRUEHEART ,  Bastien Jean Wolfgang CHEVREUX

IPC: C12P33/02 ,  C12N9/02 ,  C12N1/16

Abstract: The present invention is related to an improved method for production of 7-dehydrocholesterol (7-DHC), an important intermediate towards biotechnological production of vitamin D3 or derivatives/metabolites thereof. The invention features modified host strains expressing enzymes having improved C-5 sterol 5 desaturase activity and their use in a process for production of vitamin D3 or derivatives and/or metabolites thereof.

公开(公告)号：US20210115412A1

公开(公告)日：2021-04-22

申请号：US17050603

申请日：2019-05-21

Applicant: DSM IP ASSETS B.V.

Inventor： Christopher Mark FARRELL ,  Lisa Ann LAPRADE ,  Otto Martin LEHMANN ,  Joshua TRUEHEART

IPC: C12N9/10 ,  C12P33/00

Abstract: The present invention is related to modified sterol acyltransferase enzymes with improved activity and/or specificity towards acylation of the vitamin D3 precursor 7-dehydrocholesterol (7-DHC) to be used in biotechnological production of vitamin D3. The invention further relates to a yeast strain expressing said modified enzymes and their use in a process for production of vitamin D3 or derivatives and/or metabolites thereof.

公开(公告)号：US20240200120A1

公开(公告)日：2024-06-20

申请号：US17909867

申请日：2021-03-03

Applicant: DSM IP ASSETS B.V. ,  NOVOZYMES A/S

Inventor： Claire Véronique CHATELLE ,  Otto Martin LEHMANN

IPC: C12Q1/37

CPC classification number: C12Q1/37

Abstract: The present invention provides a method and a kit for measuring protease activity in a feed sample. The method and the kit avoids the influence of feed inhibitors present in a feed sample and thus provides a stable and accurate method for measuring protease activity in the feed sample with high sensitivity.

公开(公告)号：US20210238539A1

公开(公告)日：2021-08-05

申请号：US17050626

申请日：2019-05-21

Applicant: DSM IP Assets B.V.

Inventor： Christopher Mark FARRELL ,  Lisa Ann LAPRADE ,  Otto Martin LEHMANN ,  Joshua TRUEHEART ,  Bastien Jean Wolfgang CHEVREUX

IPC: C12N1/16 ,  C12N9/90 ,  C12P33/00

Abstract: The present invention is related to an improved method for production of 7-dehydrocholesterol (7-DHC), an important intermediate towards biotechnological production of vitamin D3 or derivatives/metabolites thereof. The invention features modified yeast strains expressing enzymes having improved C-8 sterol isomerase activity leading to increased ratios of 7-DHC in the sterol mix.

公开(公告)号：US20210095325A1

公开(公告)日：2021-04-01

申请号：US17050639

申请日：2019-05-21

Applicant: DSM IP ASSETS B.V.

Inventor： Otto Martin LEHMANN ,  Harald PICHLER ,  Holly STOLTERFOHT

IPC: C12P33/00 ,  C12N9/10 ,  C12N9/02

Abstract: The present invention is related to modified sterol acyltransferase enzymes with improved activity and/or specificity towards acylation of the vitamin D3 precursor 7-dehydrocholesterol (7-DHC) to be used in biotechnological production of vitamin D3. The invention further relates to a host strain expressing said modified enzymes and their use in a process for production of vitamin D3 or derivatives and/or metabolites thereof.