公开(公告)号：US20200248151A1

公开(公告)日：2020-08-06

申请号：US16648851

申请日：2018-09-25

Applicant: DSM IP ASSETS B.V.

Inventor： Nathalie BALCH ,  Paul BLOMQUIST ,  Reed DOTEN ,  Peter HOUSTON ,  Ethan LAM ,  Jenna MCMAHON ,  Joshua TRUEHEART ,  Celine VIAROUGE

IPC: C12N9/04 ,  C12P23/00

Abstract: The present invention is related to a novel enzymatic process for production of vitamin A alcohol (retinol) via conversion of retinal, which process includes the use of heterologous enzymes having activity as retinal reductase, particularly wherein the reaction leads to at least about 90% conversion of retinal into retinol. Said process is particularly useful for biotechnological production of vitamin A.

公开(公告)号：US20200239924A1

公开(公告)日：2020-07-30

申请号：US16648872

申请日：2018-09-25

Applicant: DSM IP ASSETS B.V.

Inventor： Nathalie BALCH ,  Paul BLOMQUIST ,  Reed DOTEN ,  Peter HOUSTON ,  Ethan LAM ,  Jenna MCMAHON ,  Joshua TRUEHEART ,  Celine VIAROUGE

IPC: C12P23/00

Abstract: The present invention is related to a novel enzymatic process for production of retinyl esters, such as in particular retinyl long chain esters, via conversion of retinol, which process includes the use of enzymes having acyltransferase activity. Said process might be used for biotechnological production of vitamin A.

公开(公告)号：US20210238539A1

公开(公告)日：2021-08-05

申请号：US17050626

申请日：2019-05-21

Applicant: DSM IP Assets B.V.

Inventor： Christopher Mark FARRELL ,  Lisa Ann LAPRADE ,  Otto Martin LEHMANN ,  Joshua TRUEHEART ,  Bastien Jean Wolfgang CHEVREUX

IPC: C12N1/16 ,  C12N9/90 ,  C12P33/00

Abstract: The present invention is related to an improved method for production of 7-dehydrocholesterol (7-DHC), an important intermediate towards biotechnological production of vitamin D3 or derivatives/metabolites thereof. The invention features modified yeast strains expressing enzymes having improved C-8 sterol isomerase activity leading to increased ratios of 7-DHC in the sterol mix.

公开(公告)号：US20200277644A1

公开(公告)日：2020-09-03

申请号：US16649771

申请日：2018-09-25

Applicant: DSM IP ASSETS B.V.

Inventor： Nathalie BALCH ,  Paul BLOMQUIST ,  Reed DOTEN ,  Peter HOUSTON ,  Ethan LAM ,  Jenna MCMAHON ,  Joshua TRUEHEART ,  Celine VIAROUGE ,  René Marcel DE JONG

IPC: C12P23/00 ,  C12N9/02

Abstract: The present invention is related to a novel enzymatic process for production of vitamin A aldehyde (retinal) via stereoselective conversion of beta-carotene which process includes the use of trans-selective enzymes having activity as beta-carotene oxidases (BCOs), in particular having preference for trans-retinal. 5 Said process is in particular useful for biotechnological production of vitamin A.

公开(公告)号：US20150104543A1

公开(公告)日：2015-04-16

申请号：US14496057

申请日：2014-09-25

Applicant: DSM IP ASSETS B.V.

Inventor： Aaron Adriaan WINKLER ,  Abraham Frederik DE HULSTER ,  Johannes Pieter VAN DIJKEN ,  Jacobus Thomas PRONK ,  Joshua TRUEHEART ,  Kevin T. MADDEN ,  Carlos Gancedo RODRIGUEZ ,  Carmen-Lisset Flores MAURIZ ,  Antonius Jeroen Adriaan VAN MARIS ,  Jacob C. HARRISON

IPC: C12N15/52 ,  A23L1/28 ,  C12P7/46

CPC classification number: C12N15/52 ,  A23L5/00 ,  A23L31/00 ,  A23V2002/00 ,  C07K14/37 ,  C12P7/46

Abstract: Improved systems for the biological production of organic acids are described.

Abstract translation: 描述了用于生物制备有机酸的改进的系统。

公开(公告)号：US20240218336A1

公开(公告)日：2024-07-04

申请号：US18439987

申请日：2024-02-13

Applicant: DSM IP ASSETS B.V.

Inventor： Nathalie BALCH ,  Paul BLOMQUIST ,  Reed DOTEN ,  Peter HOUSTON ,  Ethan LAM ,  Jenna MCMAHON ,  Joshua TRUEHEART ,  Celine VIAROUGE

IPC: C12N9/04 ,  C12P23/00

CPC classification number: C12N9/0006 ,  C12P23/00 ,  C12Y101/01105

Abstract: The present invention is related to a novel enzymatic process for production of vitamin A alcohol (retinol) via conversion of retinal, which process includes the use of heterologous enzymes having activity as retinal reductase, particularly wherein the reaction leads to at least about 90% conversion of retinal into retinol. Said process is particularly useful for biotechnological production of vitamin A.

公开(公告)号：US20210222222A1

公开(公告)日：2021-07-22

申请号：US17051610

申请日：2019-05-21

Applicant: DSM IP ASSETS B.V.

Inventor： Christopher Mark FARRELL ,  Lisa Ann LAPRADE ,  Otto Martin LEHMANN ,  Joshua TRUEHEART ,  Bastien Jean Wolfgang CHEVREUX

IPC: C12P33/02 ,  C12N9/02 ,  C12N1/16

Abstract: The present invention is related to an improved method for production of 7-dehydrocholesterol (7-DHC), an important intermediate towards biotechnological production of vitamin D3 or derivatives/metabolites thereof. The invention features modified host strains expressing enzymes having improved C-5 sterol 5 desaturase activity and their use in a process for production of vitamin D3 or derivatives and/or metabolites thereof.

公开(公告)号：US20210115412A1

公开(公告)日：2021-04-22

申请号：US17050603

申请日：2019-05-21

Applicant: DSM IP ASSETS B.V.

Inventor： Christopher Mark FARRELL ,  Lisa Ann LAPRADE ,  Otto Martin LEHMANN ,  Joshua TRUEHEART

IPC: C12N9/10 ,  C12P33/00

Abstract: The present invention is related to modified sterol acyltransferase enzymes with improved activity and/or specificity towards acylation of the vitamin D3 precursor 7-dehydrocholesterol (7-DHC) to be used in biotechnological production of vitamin D3. The invention further relates to a yeast strain expressing said modified enzymes and their use in a process for production of vitamin D3 or derivatives and/or metabolites thereof.

公开(公告)号：US20200231993A1

公开(公告)日：2020-07-23

申请号：US16647730

申请日：2018-09-25

Applicant: DSM IP ASSETS B.V.

Inventor： Nathalie BALCH ,  Paul BLOMQUIST ,  Reed DOTEN ,  Peter HOUSTON ,  Ethan LAM ,  Jenna MCMAHON ,  Joshua TRUEHEART ,  Celine VIAROUGE

IPC: C12P7/22 ,  C12N9/04 ,  C12N9/10 ,  C12P7/62

Abstract: The present invention is related to a novel enzymatic process for production of retinoids via a multi-step process, which process includes the use of heterologous enzymes having activity in a carotene-producing host cell, particularly wherein such process results in high percentage of retinoids, in trans-isoform.

公开(公告)号：US20230227862A1

公开(公告)日：2023-07-20

申请号：US18161236

申请日：2023-01-30

Applicant: DSM IP ASSETS B.V.

Inventor： Nathalie BALCH ,  Paul BLOMQUIST ,  Reed DOTEN ,  Peter HOUSTON ,  Ethan LAM ,  Jenna MCMAHON ,  Joshua TRUEHEART ,  Celine VIAROUGE

IPC: C12P7/22 ,  C12N9/04 ,  C12N9/10 ,  C12P7/62

CPC classification number: C12P7/22 ,  C12N9/0006 ,  C12N9/1029 ,  C12P7/62 ,  C12Y101/01105 ,  C12Y203/0102

Abstract: The present invention is related to a novel enzymatic process for production of retinoids via a multi-step process, which process includes the use of heterologous enzymes having activity in a carotene-producing host cell, particularly wherein such process results in high percentage of retinoids, in trans-isoform.