公开(公告)号：US20140053299A1

公开(公告)日：2014-02-20

申请号：US13771840

申请日：2013-02-20

Applicant: DSM IP Assets B.V.

Inventor： James G. METZ ,  James H. Flatt ,  Jerry M. Kuner ,  William R. Barclay

IPC: C12N15/82

CPC classification number: C12N15/8247 ,  C07K14/21 ,  C07K14/28 ,  C07K14/405 ,  C12N15/52 ,  C12P7/6427 ,  C12P7/6472

Abstract: Disclosed are the complete polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) systems from Schizochytrium, and biologically active fragments and homologues thereof. More particularly, this invention relates to nucleic acids encoding such PUFA PKS systems, to proteins and domains thereof that comprise such PUFA PKS systems, to genetically modified organisms (plants and microorganisms) comprising such PUFA PKS systems, and to methods of making and using the PUFA PKS systems disclosed herein. This invention also relates to genetically modified plants and microorganisms and methods to efficiently produce lipids enriched in various polyunsaturated fatty acids (PUFAs) as well as other bioactive molecules by manipulation of a PUFA polyketide synthase (PKS) system.

Abstract translation: 公开了来自裂殖壶菌的完全多不饱和脂肪酸（PUFA）聚酮化合物合成酶（PKS）系统及其生物活性片段及其同源物。 更具体地，本发明涉及编码这种PUFA PKS系统的核酸，其包含这种PUFA PKS系统的蛋白质和结构域到包含这种PUFA PKS系统的转基因生物（植物和微生物），以及制备和使用 本文公开的PUFA PKS系统。 本发明还涉及基因修饰的植物和微生物以及通过操作PUFA聚酮化合物合酶（PKS）系统来有效地产生富含多种不饱和脂肪酸（PUFA）以及其它生物活性分子的脂质的方法。

公开(公告)号：US20170049131A1

公开(公告)日：2017-02-23

申请号：US15344948

申请日：2016-11-07

Applicant: DSM IP Assets B.V.

Inventor： Richard B. BAILEY ,  Don Dimasi ,  Jon Milton Hansen ,  Peter J. Mirrasoul, III ,  Craig M. Ruecker ,  George T. Veeder ,  Tatsuo Kaneko ,  William R. Barclay

IPC: A23K50/80 ,  C12N1/12 ,  A23L29/00 ,  A23D9/00 ,  A23K20/158 ,  C12P7/64 ,  C11B1/10

CPC classification number: A23K50/80 ,  A23D9/00 ,  A23K20/158 ,  A23L29/065 ,  A23V2002/00 ,  C11B1/10 ,  C12N1/00 ,  C12N1/12 ,  C12P7/6409 ,  C12P7/6427 ,  C12P7/6472 ,  Y10S435/911

Abstract: The present invention provides a process for growing eukaryotic microorganisms which are capable of producing lipids, in particular lipids containing polyenoic fatty acids. The present invention also provides a process for producing eukaryotic microbial lipids.

Abstract translation: 本发明提供了一种生长真核微生物的方法，其能够产生脂质，特别是含有多烯脂肪酸的脂质。 本发明还提供了一种生产真核微生物脂质的方法。