公开(公告)号：US20140212936A1

公开(公告)日：2014-07-31

申请号：US14244466

申请日：2014-04-03

Applicant: DSM IP Assets B.V.

Inventor： Craig M. Ruecker ,  Swithwin Patrick Adu-peasah ,  Brian S. Engelhardt ,  George T Veeder, III

IPC: C11B3/00

CPC classification number: C11B3/006 ,  C11B1/02 ,  C11B3/001 ,  C12N1/06 ,  C12P7/6427 ,  C12P7/6463 ,  C12P7/6472 ,  C12P23/00 ,  C12P25/00 ,  C12P33/00

Abstract: The present invention provides a method for extracting lipids from microorganisms without using organic solvent as an extraction solvent. In particular, the present invention provides a method for extracting lipids from microorganisms by lysing cells and removing water soluble compound and/or materials by washing the lysed cell mixtures with aqueous washing solutions until a substantially non-emulsified lipid is obtained.

Abstract translation: 本发明提供一种从不使用有机溶剂作为提取溶剂的微生物提取脂质的方法。 特别地，本发明提供了一种通过裂解细胞并通过用水洗涤溶液洗涤裂解的细胞混合物除去水溶性化合物和/或物质从微生物中提取脂质直至得到基本上未乳化的脂质的方法。

公开(公告)号：US09738851B2

公开(公告)日：2017-08-22

申请号：US14244466

申请日：2014-04-03

Applicant: DSM IP Assets B.V.

Inventor： Craig M. Ruecker ,  Swithwin Patrick Adu-peasah ,  Brian S. Engelhardt ,  George T Veeder, III

IPC: C12P1/00 ,  C12P7/64 ,  C11B3/00 ,  C11B1/02 ,  C12N1/06 ,  C12P23/00 ,  C12P25/00 ,  C12P33/00

CPC classification number: C11B3/006 ,  C11B1/02 ,  C11B3/001 ,  C12N1/06 ,  C12P7/6427 ,  C12P7/6463 ,  C12P7/6472 ,  C12P23/00 ,  C12P25/00 ,  C12P33/00

Abstract: The present invention provides a method for extracting lipids from microorganisms without using organic solvent as an extraction solvent. In particular, the present invention provides a method for extracting lipids from microorganisms by lysing cells and removing water soluble compound and/or materials by washing the lysed cell mixtures with aqueous washing solutions until a substantially non-emulsified lipid is obtained.

公开(公告)号：US10329515B2

公开(公告)日：2019-06-25

申请号：US15642835

申请日：2017-07-06

Applicant: DSM IP Assets B.V.

Inventor： Craig M. Ruecker ,  Swithwin Patrick Adu-Peasah ,  Brian S. Engelhardt ,  George T Veeder, III

IPC: C12P7/64 ,  C11B3/00 ,  C11B1/02 ,  C12N1/06 ,  C12P23/00 ,  C12P25/00 ,  C12P33/00

Abstract: The present invention provides a method for extracting lipids from microorganisms without using organic solvent as an extraction solvent. In particular, the present invention provides a method for extracting lipids from microorganisms by lysing cells and removing water soluble compound and/or materials by washing the lysed cell mixtures with aqueous washing solutions until a substantially non-emulsified lipid is obtained.

公开(公告)号：US20170298288A1

公开(公告)日：2017-10-19

申请号：US15642835

申请日：2017-07-06

Applicant: DSM IP Assets B.V.

Inventor： Craig M. Ruecker ,  Swithwin Patrick Adu-Peasah ,  Brian S. Engelhardt ,  George T Veeder, III

IPC: C11B3/00 ,  C12P25/00 ,  C12P23/00 ,  C12P7/64 ,  C11B1/02 ,  C12N1/06 ,  C12P33/00

CPC classification number: C11B3/006 ,  C11B1/02 ,  C11B3/001 ,  C12N1/06 ,  C12P7/6427 ,  C12P7/6463 ,  C12P7/6472 ,  C12P23/00 ,  C12P25/00 ,  C12P33/00

Abstract: The present invention provides a method for extracting lipids from microorganisms without using organic solvent as an extraction solvent. In particular, the present invention provides a method for extracting lipids from microorganisms by lysing cells and removing water soluble compound and/or materials by washing the lysed cell mixtures with aqueous washing solutions until a substantially non-emulsified lipid is obtained.

公开(公告)号：US20170049131A1

公开(公告)日：2017-02-23

申请号：US15344948

申请日：2016-11-07

Applicant: DSM IP Assets B.V.

Inventor： Richard B. BAILEY ,  Don Dimasi ,  Jon Milton Hansen ,  Peter J. Mirrasoul, III ,  Craig M. Ruecker ,  George T. Veeder ,  Tatsuo Kaneko ,  William R. Barclay

IPC: A23K50/80 ,  C12N1/12 ,  A23L29/00 ,  A23D9/00 ,  A23K20/158 ,  C12P7/64 ,  C11B1/10

CPC classification number: A23K50/80 ,  A23D9/00 ,  A23K20/158 ,  A23L29/065 ,  A23V2002/00 ,  C11B1/10 ,  C12N1/00 ,  C12N1/12 ,  C12P7/6409 ,  C12P7/6427 ,  C12P7/6472 ,  Y10S435/911

Abstract: The present invention provides a process for growing eukaryotic microorganisms which are capable of producing lipids, in particular lipids containing polyenoic fatty acids. The present invention also provides a process for producing eukaryotic microbial lipids.

Abstract translation: 本发明提供了一种生长真核微生物的方法，其能够产生脂质，特别是含有多烯脂肪酸的脂质。 本发明还提供了一种生产真核微生物脂质的方法。