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    Modified substrates for protection of peptide-immobilized surfaces from gamma radiation degradation
    3.
    发明授权
    Modified substrates for protection of peptide-immobilized surfaces from gamma radiation degradation 有权

    公开(公告)号：US10144914B2

    公开(公告)日：2018-12-04

    申请号：US13032166

    申请日：2011-02-22

    申请人： Dana Craig Bookbinder ,  Arthur Winston Martin ,  Santona Pal ,  Christopher Bankole Shogbon ,  Jennifer L. Weber ,  Yue Zhou

    发明人： Dana Craig Bookbinder ,  Arthur Winston Martin ,  Santona Pal ,  Christopher Bankole Shogbon ,  Jennifer L. Weber ,  Yue Zhou

    IPC分类号： C12N5/00

    摘要： In the present specification and claims, three modified cell culture substrates are disclosed for protecting peptide mimetic surfaces used in cell culture technology. The methods are able to retain the functionality of the bioactive species conjugated to the surface. In particular, a vitronectin peptide fragment Ac-Lys-Gly-Gly-Pro-Gln-Val-Thr-Arg-Gly-Asp-Val-Phe-Thr-Met-Pro-NH2 was able to facilitate growth and proliferation of undifferentiated human embryonic stem cells after stabilization using 3 different modified cell culture substrates for of protection against gamma irradiation. The modified substrates disclosed in this invention are (i) covalent attachment of the antioxidant molecules (via blocking step); (ii) The use of coatings consisting of anti-oxidants and stabilizers comprising glycoprotiens, sugars, carbohydrates, poly(amino acids), peptides and hydrophilic polymers; and (iii) a third method wherein the same methionine contained in the bioactive sequence used to facilitate growth and proliferation of human embryonic stem cells was also used as a sacrificial coating to protect the same conjugated sequence against damage by gamma irradiation.

    MODIFIED SUBSTRATES FOR PROTECTION OF PEPTIDE-IMMOBILIZED SURFACES FROM GAMMA RADIATION DEGRADATION
    4.
    发明申请
    MODIFIED SUBSTRATES FOR PROTECTION OF PEPTIDE-IMMOBILIZED SURFACES FROM GAMMA RADIATION DEGRADATION 审中-公开
    用于保护肽固定表面的改性基质从伽马辐射降解

    公开(公告)号：US20110207219A1

    公开(公告)日：2011-08-25

    申请号：US13032166

    申请日：2011-02-22

    申请人： Dana Craig Bookbinder ,  Arthur Winston Martin ,  Santona Pal ,  Christopher Bankole Shogbon ,  Jennifer L. Weber ,  Yue Zhou

    发明人： Dana Craig Bookbinder ,  Arthur Winston Martin ,  Santona Pal ,  Christopher Bankole Shogbon ,  Jennifer L. Weber ,  Yue Zhou

    IPC分类号： C12N5/071

    CPC分类号： C12N5/0068 ,  C12N2533/20 ,  C12N2533/50

    摘要： In the present specification and claims, three modified cell culture substrates are disclosed for protecting peptide mimetic surfaces used in cell culture technology. The methods are able to retain the functionality of the bioactive species conjugated to the surface. In particular, a vitronectin peptide fragment Ac-Lys-Gly-Gly-Pro-Gln-Val-Thr-Arg-Gly-Asp-Val-Phe-Thr-Met-Pro-NH2 was able to facilitate growth and proliferation of undifferentiated human embryonic stem cells after stabilization using 3 different modified cell culture substrates for of protection against gamma irradiation. The modified substrates disclosed in this invention are (i) covalent attachment of the antioxidant molecules (via blocking step); (ii) The use of coatings consisting of anti-oxidants and stabilizers comprising glycoprotiens, sugars, carbohydrates, poly(amino acids), peptides and hydrophilic polymers; and (iii) a third method wherein the same methionine contained in the bioactive sequence used to facilitate growth and proliferation of human embryonic stem cells was also used as a sacrificial coating to protect the same conjugated sequence against damage by gamma irradiation.

    摘要翻译： 在本说明书和权利要求书中，公开了三种修饰的细胞培养基质，用于保护细胞培养技术中使用的肽模拟表面。 该方法能够保留与表面缀合的生物活性物质的功能。 特别地，玻连蛋白肽片段Ac-Lys-Gly-Gly-Pro-Gln-Val-Thr-Arg-Gly-Asp-Val-Phe-Thr-Met-Pro-NH2能够促进未分化人的生长和增殖 使用3种不同的修饰的细胞培养物底物进行稳定后的胚胎干细胞以防止γ照射。 本发明公开的改性底物是（i）抗氧化剂分子的共价连接（通过封闭步骤）; （ii）使用包含抗氧化剂和稳定剂的涂层，其包含糖调节剂，糖，碳水化合物，聚（氨基酸），肽和亲水性聚合物; 和（iii）第三种方法，其中用于促进人类胚胎干细胞生长和增殖的生物活性序列中所含的相同甲硫氨酸也用作牺牲涂层以保护相同缀合序列免受γ照射的损伤。

    SWELLABLE (METH)ACRYLATE SURFACES FOR CULTURING CELLS IN CHEMICALLY DEFINED MEDIA
    7.
    发明申请
    SWELLABLE (METH)ACRYLATE SURFACES FOR CULTURING CELLS IN CHEMICALLY DEFINED MEDIA 有权
    用于化学定义培养基培养细胞的（甲基）丙烯酸酯表面

    公开(公告)号：US20090191632A1

    公开(公告)日：2009-07-30

    申请号：US12362974

    申请日：2009-01-30

    申请人： Andrei G. Fadeev ,  Jennifer Gehman ,  Arthur Winston Martin ,  Zara Melkoumian ,  Christopher B. Shogbon ,  David Michael Weber ,  Yue Zhou

    发明人： Andrei G. Fadeev ,  Jennifer Gehman ,  Arthur Winston Martin ,  Zara Melkoumian ,  Christopher B. Shogbon ,  David Michael Weber ,  Yue Zhou

    IPC分类号： C12N5/02

    CPC分类号： C12N5/0606 ,  C12N5/0068 ,  C12N2533/30 ,  C12N2533/50

    摘要： Synthetic surfaces capable of supporting culture of undifferentiated human embryonic stem cells in a chemically defined medium include a swellable (meth)acrylate layer and a peptide conjugated to the swellable (meth)acrylate layer. The swellable (meth)acrylate layer may be formed by polymerizing monomers in a composition that includes hydroxyethyl methacrylate, 2-carboxyehylacrylate, and tetra(ethylene glycol) dimethacrylate. The conjugated peptide may include an amino acid sequence of XaanProGlnValThrArgGlyAspValPheThrMetPro, where n is an integer from 0 to 3 and where Xaa is any amino acid. Further, disclosed herein is a swellable (meth)acrylate synthetic surface which can be sterilized by gamma irradiation.

    摘要翻译： 能够支持未分化的人类胚胎干细胞培养在化学成分确定的培养基中的合成表面包括可溶胀的（甲基）丙烯酸酯层和与可溶胀（甲基）丙烯酸酯层共轭的肽。 可溶胀的（甲基）丙烯酸酯层可以通过使包含甲基丙烯酸羟乙酯，2-羧基丙烯酸烯丙酯和四（乙二醇）二甲基丙烯酸酯的组合物中的单体聚合而形成。 缀合肽可以包括XananProGlnValThrArgGlyAspValPheThrMetPro的氨基酸序列，其中n是0至3的整数，并且其中Xaa是任何氨基酸。 此外，本文公开了可通过γ辐射灭菌的可溶胀（甲基）丙烯酸酯合成表面。

    Swellable (meth)acrylate surfaces for culturing cells in chemically defined media
    8.
    发明授权
    Swellable (meth)acrylate surfaces for culturing cells in chemically defined media 有权
    可溶胀的（甲基）丙烯酸酯表面用于在化学上限定的培养基中培养细胞

    公开(公告)号：US08329469B2

    公开(公告)日：2012-12-11

    申请号：US12362974

    申请日：2009-01-30

    申请人： Andrei G. Fadeev ,  Jennifer Gehman ,  Arthur Winston Martin ,  Zara Melkoumian ,  Christopher B. Shogbon ,  David Michael Weber ,  Yue Zhou

    发明人： Andrei G. Fadeev ,  Jennifer Gehman ,  Arthur Winston Martin ,  Zara Melkoumian ,  Christopher B. Shogbon ,  David Michael Weber ,  Yue Zhou

    IPC分类号： C12N5/00 ,  C12N11/00

    CPC分类号： C12N5/0606 ,  C12N5/0068 ,  C12N2533/30 ,  C12N2533/50

    摘要： Synthetic surfaces capable of supporting culture of undifferentiated human embryonic stem cells in a chemically defined medium include a swellable (meth)acrylate layer and a peptide conjugated to the swellable (meth)acrylate layer. The swellable (meth)acrylate layer may be formed by polymerizing monomers in a composition that includes hydroxyethyl methacrylate, 2-carboxyehylacrylate, and tetra(ethylene glycol) dimethacrylate. The conjugated peptide may include an amino acid sequence of XaanProGlnValThrArgGlyAspValPheThrMetPro, where n is an integer from 0 to 3 and where Xaa is any amino acid. Further, disclosed herein is a swellable (meth)acrylate synthetic surface which can be sterilized by gamma irradiation.

    摘要翻译： 能够支持未分化的人类胚胎干细胞培养在化学成分确定的培养基中的合成表面包括可溶胀的（甲基）丙烯酸酯层和与可溶胀的（甲基）丙烯酸酯层共轭的肽。 可溶胀的（甲基）丙烯酸酯层可以通过使包含甲基丙烯酸羟乙酯，2-羧基丙烯酸烯丙酯和四（乙二醇）二甲基丙烯酸酯的组合物中的单体聚合而形成。 缀合肽可以包括XananProGlnValThrArgGlyAspValPheThrMetPro的氨基酸序列，其中n是0至3的整数，并且其中Xaa是任何氨基酸。 此外，本文公开了可通过γ辐射灭菌的可溶胀（甲基）丙烯酸酯合成表面。

    Ranking non-product documents
    10.
    发明授权
    Ranking non-product documents 有权
    排列非产品文件

    公开(公告)号：US08666914B1

    公开(公告)日：2014-03-04

    申请号：US13113979

    申请日：2011-05-23

    申请人： Benoit J. Dupin ,  Dimitris Margaritis ,  Steve L. Martin ,  Diane Hernek ,  Nicholas E. Matsakis ,  Yue Zhou

    发明人： Benoit J. Dupin ,  Dimitris Margaritis ,  Steve L. Martin ,  Diane Hernek ,  Nicholas E. Matsakis ,  Yue Zhou

    IPC分类号： G06F15/18

    CPC分类号： G06F17/30687

    摘要： Systems, methods, and apparatus are provided for determining relevance of documents to queries. An optimized relevance function is configured to determine a relevance value of documents of a first type that are linked to documents of a second type. The relevance function is optimized to satisfy certain criteria. According to one criterion, a relevance value produced by the optimized relevance function, when invoked for documents of the first type, should have a locally maximal degree of fit to the results of the existing relevance function for the sample training documents of the second type. An assessed degree of fit of a document can be increased or decreased to arrive at an optimized relevance function that ranks the documents of the first type in a desired position relative to documents of the second type in search results. The degree of fit can be assessed by a user-provided objective function.

    摘要翻译： 提供了系统，方法和装置，用于确定文档与查询的相关性。 优化的相关性功能被配置为确定链接到第二类型的文档的第一类型的文档的相关性值。 相关性功能被优化以满足某些标准。 根据一个标准，当针对第一类型的文档调用时，由优化的相关性函数产生的相关性值应当具有对于第二类型的样本训练文档的现有相关性函数的结果的局部最大拟合度。 可以增加或减少文档的评估的适合度以获得优化的相关性功能，其将相对于搜索结果中的第二类型的文档排列在期望位置的第一类型的文档。 可以通过用户提供的目标函数来评估拟合度。