公开(公告)号：US20060065531A1

公开(公告)日：2006-03-30

申请号：US11234054

申请日：2005-09-22

申请人： Daniel Smolko ,  Paul Swanson ,  Dalibor Hodko ,  David Canter ,  Robert Haigis ,  Tricia Patterson

发明人： Daniel Smolko ,  Paul Swanson ,  Dalibor Hodko ,  David Canter ,  Robert Haigis ,  Tricia Patterson

IPC分类号： C07K1/26 ,  G01N27/447

CPC分类号： C12Q1/6825 ,  C12Q2565/607

摘要： Methods for the transport and hybridization of a nucleic acid on an electrode device by providing a low conductivity buffer with a reducing agent to the device. The low conductivity buffer may also contain a zwitterion. A current and voltage is applied to a location of the device to effect electrophoretic transportation of the nucleic acid towards the location. The nucleic acid is then hybridized to a nucleic probed located at the location. The reducing agent in the low conductivity buffer may also be acting as a chaotropic agent.

摘要翻译： 通过向装置提供具有还原剂的低电导率缓冲液来将核酸在电极装置上的运输和杂交的方法。 低电导率缓冲液也可以含有两性离子。 将电流和电压施加到装置的位置以实现核酸向该位置的电泳运输。 然后将核酸与位于该位置的核酸探针杂交。 低电导率缓冲液中的还原剂也可用作离液剂。

公开(公告)号：US20100133118A1

公开(公告)日：2010-06-03

申请号：US12624321

申请日：2009-11-23

申请人： Ronald G. Sosnowski ,  Gabriel Baru Fassio ,  Zuxu Yao ,  Robert Haigis ,  Tao Ye

发明人： Ronald G. Sosnowski ,  Gabriel Baru Fassio ,  Zuxu Yao ,  Robert Haigis ,  Tao Ye

IPC分类号： C12Q1/68 ,  G01N27/26

CPC分类号： C12Q1/6825 ,  C12Q2563/113 ,  C12Q2525/107

摘要： In accordance with the present invention, there are provided systems for detecting hybridization of nucleic acids using electrochemical methods having improved sensitivity. Such systems include an electrode having a variably charged oligonucleotide probe and a redox probe. In some embodiments, the systems may further include a binding nexus having an immobilized reporter oligonucleotide probe, which hybridizes to a target nucleic acid sequence. The reporter oligonucleotide probe may be naturally charged, uncharged, or either partially negatively or positively charged. Further provided are methods for detecting the presence of a nucleic acid sequence of interest in a sample.

摘要翻译： 根据本发明，提供了使用具有改进的灵敏度的电化学方法来检测核酸杂交的系统。 这样的系统包括具有可变电荷的寡核苷酸探针和氧化还原探针的电极。 在一些实施方案中，系统可以进一步包括具有与靶核酸序列杂交的固定的报道寡核苷酸探针的结合核苷酸。 报道寡核苷酸探针可以是天然带电的，不带电的，或部分负电荷或带正电荷的。 还提供了用于检测样品中感兴趣的核酸序列的存在的方法。

公开(公告)号：US06887362B2

公开(公告)日：2005-05-03

申请号：US10072660

申请日：2002-02-06

申请人： Ying Huang ,  Karla Ewalt ,  Robert Haigis ,  Anita Forster ,  Michael K. Krihak

发明人： Ying Huang ,  Karla Ewalt ,  Robert Haigis ,  Anita Forster ,  Michael K. Krihak

IPC分类号： B01L3/00 ,  B03C5/02 ,  G01N27/26

CPC分类号： B01L3/502761 ,  B01J2219/00274 ,  B01L2200/0668 ,  B01L2200/10 ,  B01L2300/0877 ,  B01L2400/0415 ,  B03C5/026

摘要： This invention relates to devices and methods for performing active, multi-step molecular and biological sample preparation and diagnostic analyses employing immunochemical techniques. It relates generally to bioparticle separation, bioparticle enrichment, and electric field-mediated immunochemical detection on active electronic matrix devices utilizing AC and DC electric fields. More specifically, the invention relates to devices and methods for sample preparation/manipulation, immunoimmobilization, and immunoassays, all of which can be conducted on one or more active electronic chip devices within a single system. These manipulations are useful in a variety of applications, including, for example, detection of pathogenic bacteria and biological warfare agents, point-of-care diagnostics, food or medical product quality control assays, and other biological assays.

摘要翻译： 本发明涉及使用免疫化学技术进行活性多步分子和生物样品制备和诊断分析的装置和方法。 它通常涉及利用AC和DC电场的有源电子矩阵装置上的生物颗粒分离，生物颗粒富集和电场介导的免疫化学检测。 更具体地，本发明涉及用于样品制备/操作，免疫移植和免疫测定的装置和方法，所有这些装置和方法都可以在单个系统内的一个或多个有源电子芯片装置上进​​行。 这些操作可用于各种应用，包括例如检测病原菌和生物战剂，点护理诊断，食品或医药产品质量控制测定和其他生物测定。