System and method for automatically determining optimal excitation and emission wavelengths of a fluorophore
    2.
    发明授权
    System and method for automatically determining optimal excitation and emission wavelengths of a fluorophore 有权
    自动确定荧光团的最佳激发和发射波长的系统和方法

    公开(公告)号:US08723139B2

    公开(公告)日:2014-05-13

    申请号:US13156877

    申请日:2011-06-09

    IPC分类号: G01N21/64

    摘要: A system for performing spectrofluorometry of a fluorophore sample is provided. The system includes an input module that receives user input corresponding to spectrofluorometer settings. A control module transmits control signals for controlling the spectrofluorometer during respective wavelength scans of a fluorophore sample and a blank sample. The control signals provide for automatic execution of the wavelength scans over an excitation wavelength range and an emission wavelength range. A signal-to-background determination module automatically determines multiple signal-to-background ratios based on fluorescence measurements of the fluorophore sample and the blank sample received from the spectrofluorometer. A signal-to-background analysis module automatically determines the maximum signal-to-background ratio from the multiple signal-to-background ratios.

    摘要翻译: 提供了一种用于进行荧光团样品的分光荧光测定的系统。 该系统包括输入模块,其接收对应于分光荧光计设置的用户输入。 控制模块在荧光团样品和空白样品的相应波长扫描期间传输用于控制分光荧光计的控制信号。 控制信号提供在激发波长范围和发射波长范围内自动执行波长扫描。 信号对背景确定模块基于荧光团样品和从分光荧光计接收的空白样品的荧光测量自动确定多个信号与背景比。 信号到背景分析模块自动确定从多个信号到背景比的最大信号对背景比。

    System and Method for Automatically Determining Optimal Excitation and Emission Wavelengths of a Fluorophore
    3.
    发明申请
    System and Method for Automatically Determining Optimal Excitation and Emission Wavelengths of a Fluorophore 有权
    用于自动确定荧光团的最佳激发和发射波长的系统和方法

    公开(公告)号:US20120313010A1

    公开(公告)日:2012-12-13

    申请号:US13156877

    申请日:2011-06-09

    IPC分类号: G01J1/58

    摘要: A system for performing spectrofluorometry of a fluorophore sample is provided. The system includes an input module that receives user input corresponding to spectrofluorometer settings. A control module transmits control signals for controlling the spectrofluorometer during respective wavelength scans of a fluorophore sample and a blank sample. The control signals provide for automatic execution of the wavelength scans over an excitation wavelength range and an emission wavelength range. A signal-to-background determination module automatically determines multiple signal-to-background ratios based on fluorescence measurements of the fluorophore sample and the blank sample received from the spectrofluorometer. A signal-to-background analysis module automatically determines the maximum signal-to-background ratio from the multiple signal-to-background ratios.

    摘要翻译: 提供了一种用于进行荧光团样品的分光荧光测定的系统。 该系统包括输入模块,其接收对应于分光荧光计设置的用户输入。 控制模块在荧光团样品和空白样品的相应波长扫描期间传输用于控制分光荧光计的控制信号。 控制信号提供在激发波长范围和发射波长范围内自动执行波长扫描。 信号对背景确定模块基于荧光团样品和从分光荧光计接收的空白样品的荧光测量自动确定多个信号与背景比。 信号到背景分析模块自动确定从多个信号到背景比的最大信号对背景比。