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    Methods and compositions for gene silencing
    1.
    发明申请
    Methods and compositions for gene silencing 审中-公开
    用于基因沉默的方法和组合物

    公开(公告)号：US20070130653A1

    公开(公告)日：2007-06-07

    申请号：US11455073

    申请日：2006-06-16

    申请人： David Baulcombe ,  Enno Krebbers ,  William Hitz ,  Kimberly Glassman ,  Milo Aukerman ,  Robert Williams ,  Byung-Chun Yoo

    发明人： David Baulcombe ,  Enno Krebbers ,  William Hitz ,  Kimberly Glassman ,  Milo Aukerman ,  Robert Williams ,  Byung-Chun Yoo

    IPC分类号： A01H1/00 ,  C07H21/02 ,  C12N15/82 ,  C07H21/04 ,  C12N5/04

    CPC分类号： C12N15/8218 ,  C07H21/02 ,  C07H21/04 ,  C12N2310/14

    摘要： Methods and compositions are provided for reducing the level of expression of a target polynucleotide in an organism. The methods and compositions selectively silence the target polynucleotide through the expression of a chimeric polynucleotide comprising the target for a sRNA (the trigger sequence) operably linked to a sequence corresponding to all or part of the gene or genes to be silenced. In this manner, the final target of silencing is an endogenous gene in the organism in which the chimeric polynucleotide is expressed. In a further embodiment, the miRNA target is that of a heterologous miRNA or siRNA, the latter of which is coexpressed in the cells at the appropriate developmental stage to provide silencing of the final target when and where desired. In a further embodiment, the final target may be a gene in a second organism, such as a plant pest, that feeds upon the organism containing the chimeric gene or genes. Compositions further comprise vectors, seeds, grain, cells, and organisms, including plants and plant cells, comprising the chimeric polynucleotide of the invention.

    摘要翻译： 提供了用于降低生物体中靶多核苷酸表达水平的方法和组合物。 所述方法和组合物通过表达嵌合多核苷酸来选择性沉默靶多核苷酸，所述嵌合多核苷酸包含可操作地连接到对应于待沉默的基因或基因的全部或部分的序列的sRNA（触发序列）的靶。 以这种方式，沉默的最终靶标是其中表达嵌合多核苷酸的生物体中的内源基因。 在另一个实施方案中，miRNA靶是异源miRNA或siRNA的靶标，其后者在合适的发育阶段在细胞中共表达，以在需要时和期望时提供最终靶的沉默。 在另一个实施方案中，最终靶可以是在含有嵌合基因或基因的生物体上进食的第二生物体如植物害虫中的基因。 组合物还包含载体，种子，谷粒，细胞和生物，包括植物和植物细胞，其包含本发明的嵌合多核苷酸。

    DROUGHT TOLERANT PLANTS AND RELATED CONSTRUCTS AND METHODS INVOLVING GENES ENCODING MIR827
    5.
    发明申请
    DROUGHT TOLERANT PLANTS AND RELATED CONSTRUCTS AND METHODS INVOLVING GENES ENCODING MIR827 审中-公开
    耐旱植物及相关建议及涉及编码MIR827基因的方法

    公开(公告)号：US20120117687A1

    公开(公告)日：2012-05-10

    申请号：US13356172

    申请日：2012-01-23

    申请人： Milo Aukerman ,  Wonkeun Park

    发明人： Milo Aukerman ,  Wonkeun Park

    IPC分类号： A01H5/00 ,  C12N15/82

    CPC分类号： C12N15/113 ,  C12N15/8218 ,  C12N15/8273

    摘要： Isolated polynucleotides and polypeptides and recombinant DNA constructs useful for conferring drought tolerance, compositions (such as plants or seeds) comprising these recombinant DNA constructs, and methods utilizing these recombinant DNA constructs. The recombinant DNA construct comprises a polynucleotide operably linked to a promoter that is functional in a plant, wherein said polynucleotide encodes a miR827 microRNA.

    摘要翻译： 用于赋予耐旱性的分离的多核苷酸和多肽和重组DNA构建体，包含这些重组DNA构建体的组合物（例如植物或种子），以及利用这些重组DNA构建体的方法。 重组DNA构建体包含可操作地连接于在植物中有功能的启动子的多核苷酸，其中所述多核苷酸编码miR827微小RNA。

    DROUGHT TOLERANT PLANTS AND RELATED CONSTRUCTS AND METHODS INVOLVING GENES ENCODING miR827
    6.
    发明申请
    DROUGHT TOLERANT PLANTS AND RELATED CONSTRUCTS AND METHODS INVOLVING GENES ENCODING miR827 审中-公开
    耐旱植物及相关建议及涉及编码miR827的基因的方法

    公开(公告)号：US20090165168A1

    公开(公告)日：2009-06-25

    申请号：US12340890

    申请日：2008-12-22

    申请人： Milo Aukerman ,  Wonkeun Park

    发明人： Milo Aukerman ,  Wonkeun Park

    IPC分类号： C12N15/82 ,  A01H5/00

    CPC分类号： C12N15/113 ,  C12N15/8218 ,  C12N15/8273

    摘要： Isolated polynucleotides and polypeptides and recombinant DNA constructs useful for conferring drought tolerance, compositions (such as plants or seeds) comprising these recombinant DNA constructs, and methods utilizing these recombinant DNA constructs. The recombinant DNA construct comprises a polynucleotide operably linked to a promoter that is functional in a plant, wherein said polynucleotide encodes a miR827 microRNA.

    摘要翻译： 用于赋予耐旱性的分离的多核苷酸和多肽和重组DNA构建体，包含这些重组DNA构建体的组合物（例如植物或种子），以及利用这些重组DNA构建体的方法。 重组DNA构建体包含可操作地连接于在植物中有功能的启动子的多核苷酸，其中所述多核苷酸编码miR827微小RNA。

    Gene silencing
    8.
    发明申请
    Gene silencing 审中-公开
    基因沉默

    公开(公告)号：US20050138689A1

    公开(公告)日：2005-06-23

    申请号：US10963394

    申请日：2004-10-12

    申请人： Milo Aukerman

    发明人： Milo Aukerman

    IPC分类号： A01H1/00 ,  A01H5/00 ,  C07H21/04 ,  C12N5/04 ,  C12N15/82

    CPC分类号： C12N15/8201 ,  C12N15/8216 ,  C12N15/8218 ,  C12N15/827

    摘要： The invention provides methods and compositions useful in target sequence suppression and target sequence validation. The invention provides polynucleotide constructs useful for gene silencing, as well as cells, plants and seeds comprising the polynucleotides. The invention also provides a method for using microRNA to silence a target sequence.

    摘要翻译： 本发明提供了可用于靶序列抑制和靶序列验证的方法和组合物。 本发明提供了可用于基因沉默的多核苷酸构建体，以及包含多核苷酸的细胞，植物和种子。 本发明还提供了使用微小RNA沉默靶序列的方法。