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公开(公告)号：US5567598A
公开(公告)日：1996-10-22
申请号：US25899
申请日：1993-03-03
CPC分类号： C12Q1/04 , C12Q1/18 , C12Q2304/44 , Y10S435/968 , Y10S436/80
摘要： This invention presents methods for detection and evaluation of metabolic activity of microorganisms based upon their ability to consume dissolved oxygen. The methods utilize a fluorescence detection system which makes use of the sensitivity of the fluorescent emission of certain compounds to the presence of oxygen, which quenches (diminishes) the compound's fluorescent emission in a concentration dependent manner. Respiring microorganisms will affect the oxygen concentration of a liquid medium in which they are immersed. Thus, this invention provides a convenient system to gather information on the presence, identification and metabolic activity of microorganisms by determining their effect on the oxygen concentration of the media in which they are present.
摘要翻译： 本发明提供了基于其消耗溶解氧的能力来检测和评估微生物的代谢活性的方法。 该方法利用荧光检测系统，其利用某些化合物的荧光发射对氧的存在的灵敏度，其以浓度依赖性方式猝灭（减少）化合物的荧光发射。 呼吸微生物将影响浸入其中的液体介质的氧浓度。 因此，本发明提供了一种方便的系统，通过确定它们对存在的微生物的存在，鉴定和代谢活性的影响，其对它们所存在的培养基的氧浓度的影响。
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2.发明授权
公开(公告)号：US5163441A
公开(公告)日：1992-11-17
申请号：US627295
申请日：1990-12-14
CPC分类号： A61B10/02 , A61F13/38 , C12M33/02 , C12M45/22 , A61B10/0096 , G01N2001/028
摘要： A microbiological culture collection and transport device maintains viable organisms for periods of time longer than possible with existing sampling devices. It also allows recovery of detectable antigen at levels not achievable with conventional swabs. The new device has a sterile swabbing tip made with a non-toxic polyurethane foam having open cells at its exposed surface. It does not require a transport medium and can be used dry. The device may further include a sample inoculator to distribute organisms collected onto a solid or semi solid medium. Methods for collecting and transporting microbiological specimens and for recovering detectable antigen are also described.
摘要翻译： 微生物培养物收集和运输装置在现有采样装置的时间长的时间内维持活生物体。 它还允许以常规拭子不能达到的水平回收可检测的抗原。 新装置具有无菌拭子尖端，其在其暴露表面上具有开放细胞的无毒聚氨酯泡沫塑料制成。 它不需要运输介质，可以使用干燥。 该装置可以进一步包括样品接种器以将收集的生物体分布到固体或半固体培养基上。 还描述了收集和运送微生物标本和恢复可检测抗原的方法。
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公开(公告)号：US5716798A
公开(公告)日：1998-02-10
申请号：US926729
申请日：1992-09-22
CPC分类号： C12M23/08 , C12M23/12 , C12M41/36 , Y10S436/805 , Y10S436/905 , Y10S436/909
摘要： This invention describes a method for the rapid identification of the presence of microorganisms in a sample. Briefly, in the method of this invention the sample container is divided into a plurality of discrete zones, each of which can be separately monitored for microbial presence. When a sample is placed into this container, detection is simplified as the volume monitored is low (as compared with the sample); since microbial detection is a concentration dependent phenomenon, the speed with which the presence of microbial contamination can be detected is increased.
摘要翻译： 本发明描述了用于快速鉴定样品中微生物存在的方法。 简而言之，在本发明的方法中，样品容器被分成多个离散的区域，每个离散区域可以单独地监测微生物存在。 当样品放入该容器时，由于监测的体积较低（与样品相比），因此可以简化检测。 由于微生物检测是浓度依赖现象，因此可以检测到微生物污染的存在的速度。
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公开(公告)号：US5091316A
公开(公告)日：1992-02-25
申请号：US508506
申请日：1990-04-11
CPC分类号： A61B10/02 , A61F13/38 , C12M33/02 , C12M45/22 , A61B10/0096 , G01N2001/028
摘要： A microbiological culture collection and transport device maintains viable organisms for periods of time longer than possible with existing sampling devices. It also allows recovery of detectable antigen at levels not achievable with conventional swabs. The new device has a sterile swabbing tip made with a non-toxic polyurethane foam having open cells at its exposed surface. It does not require a transport medium and can be used dry. The device may further include a sample inoculator to distribute organisms collected onto a solid or semi solid medium. Methods for collecting and transporting microbiological specimens and for recovering detectable antigen are also described.
摘要翻译： 微生物培养物收集和运输装置在现有采样装置的时间长的时间内维持活生物体。 它还允许以常规拭子不能达到的水平回收可检测的抗原。 新装置具有无菌拭子尖端，其在其暴露表面上具有开放细胞的无毒聚氨酯泡沫塑料制成。 它不需要运输介质，可以使用干燥。 该装置可以进一步包括样品接种器以将收集的生物体分布到固体或半固体培养基上。 还描述了收集和运送微生物标本和恢复可检测抗原的方法。
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公开(公告)号：US06900030B2
公开(公告)日：2005-05-31
申请号：US10116777
申请日：2002-04-04
申请人： J. Bruce Pitner , John Jacob Hemperly , Richard D. Guarino , Magdalena Wodnicka , David T. Stitt , Gregory J. Burrell , Timothy G. Foley, Jr. , Patrick Shawn Beaty
发明人： J. Bruce Pitner , John Jacob Hemperly , Richard D. Guarino , Magdalena Wodnicka , David T. Stitt , Gregory J. Burrell , Timothy G. Foley, Jr. , Patrick Shawn Beaty
CPC分类号： C12Q1/18 , C12Q1/04 , C12Q2304/44 , G06F19/00 , Y02A90/26
摘要： The present invention relates to methods for detection and evaluation of metabolic activity of eukaryotic and/or prokaryotic cells based upon their ability to consume dissolved oxygen. The methods utilize a luminescence detection system which makes use of the sensitivity of the luminescent emission of certain compounds to the presence of oxygen, which quenches (diminishes) the compound's luminescent emission in a concentration dependent manner. Respiring eukaryotic and/or prokaryotic cells will affect the oxygen concentration of a liquid medium in which they are immersed. Thus, this invention provides a convenient system to gather information on the presence, identification, quantification and cytotoxic activity of eukaryotic and/or prokaryotic cells by determining their effect on the oxygen concentration of the media in which they are present.
摘要翻译： 本发明涉及基于其消耗溶解氧的能力来检测和评价真核细胞和/或原核细胞的代谢活性的方法。 该方法利用了发光检测系统，其利用某些化合物的发光发射对氧的存在的敏感性，其以浓度依赖性方式猝灭（减少）化合物的发光发射。 呼吸真核细胞和/或原核细胞将影响浸入其中的液体培养基的氧浓度。 因此，本发明提供了一种方便的系统，用于通过确定它们对存在的介质的氧浓度的影响来收集关于真核细胞和/或原核细胞的存在，鉴定，定量和细胞毒活性的信息。
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公开(公告)号：US06395506B1
公开(公告)日：2002-05-28
申请号：US09342720
申请日：1999-06-29
申请人： J. Bruce Pitner , John Jacob Hemperly , Richard D. Guarino , Magdalena Wodnicka , David T. Stitt , Gregory J. Burrell , Timothy G. Foley, Jr. , Patrick Shawn Beaty
发明人： J. Bruce Pitner , John Jacob Hemperly , Richard D. Guarino , Magdalena Wodnicka , David T. Stitt , Gregory J. Burrell , Timothy G. Foley, Jr. , Patrick Shawn Beaty
IPC分类号： C12Q118
CPC分类号： C12Q1/18 , C12Q1/04 , C12Q2304/44 , G06F19/00 , Y02A90/26
摘要： The present invention relates to methods for detection and evaluation of metabolic activity of eukaryotic and/or prokaryotic cells based upon their ability to consume dissolved oxygen. The methods utilize a luminescence detection system which makes use of the sensitivity of the luminescent emission of certain compounds to the presence of oxygen, which quenches (diminishes) the compound's luminescent emission in a concentration dependent manner. Respiring eukaryotic and/or prokaryotic cells will affect the oxygen concentration of a liquid medium in which they are immersed. Thus, this invention provides a convenient system to gather information on the presence, identification, quantification and cytotoxic activity of eukaryotic and/or prokaryotic cells by determining their effect on the oxygen concentration of the media in which they are present.
摘要翻译： 本发明涉及基于其消耗溶解氧的能力来检测和评价真核细胞和/或原核细胞的代谢活性的方法。 该方法利用了发光检测系统，其利用某些化合物的发光发射对氧的存在的敏感性，其以浓度依赖性方式猝灭（减少）化合物的发光发射。 呼吸真核细胞和/或原核细胞将影响浸入其中的液体培养基的氧浓度。 因此，本发明提供了一种方便的系统，用于通过确定它们对存在的介质的氧浓度的影响来收集关于真核细胞和/或原核细胞的存在，鉴定，定量和细胞毒活性的信息。
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公开(公告)号：US07338778B2
公开(公告)日：2008-03-04
申请号：US10957802
申请日：2004-10-04
申请人： J. Bruce Pitner , John Jacob Hemperly , Richard D. Guarino , Magdalena Wodnicka , David T. Stitt , Gregory J. Burrell , Timothy G. Foley, Jr. , Patrick Shawn Beaty
发明人： J. Bruce Pitner , John Jacob Hemperly , Richard D. Guarino , Magdalena Wodnicka , David T. Stitt , Gregory J. Burrell , Timothy G. Foley, Jr. , Patrick Shawn Beaty
IPC分类号： C12Q1/04
CPC分类号： C12Q1/18 , C12Q1/04 , C12Q2304/44 , G06F19/00 , Y02A90/26
摘要： The present invention relates to methods for detection and evaluation of metabolic activity of eukaryotic and/or prokaryotic cells based upon their ability to consume dissolved oxygen. The methods utilize a luminescence detection system which makes use of the sensitivity of the luminescent emission of certain compounds to the presence of oxygen, which quenches (diminishes) the compound's luminescent emission in a concentration dependent manner. Respiring eukaryotic and/or prokaryotic cells will affect the oxygen concentration of a liquid medium in which they are immersed. Thus, this invention provides a convenient system to gather information on the presence, identification, quantification and cytotoxic activity of eukaryotic and/or prokaryotic cells by determining their effect on the oxygen concentration of the media in which they are present.
摘要翻译： 本发明涉及基于其消耗溶解氧的能力来检测和评价真核细胞和/或原核细胞的代谢活性的方法。 该方法利用了发光检测系统，其利用某些化合物的发光发射对氧的存在的敏感性，其以浓度依赖性方式猝灭（减少）化合物的发光发射。 呼吸真核细胞和/或原核细胞将影响浸入其中的液体培养基的氧浓度。 因此，本发明提供了一种方便的系统，用于通过确定它们对存在的介质的氧浓度的影响来收集关于真核细胞和/或原核细胞的存在，鉴定，定量和细胞毒活性的信息。
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