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6 条记录 (耗时 0.023 秒)

    Expression of fusion polypeptides transported out of the cytoplasm without leader sequences
    5.
    发明授权
    Expression of fusion polypeptides transported out of the cytoplasm without leader sequences 失效
    从细胞质中转移出的融合多肽的表达无前导序列

    公开(公告)号：US5914254A

    公开(公告)日：1999-06-22

    申请号：US854811

    申请日：1997-05-12

    申请人： Desmond Mascarenhas ,  Yang Zhang ,  Pamela S. Olson ,  David R. Olsen ,  Pedro A. Cohen ,  Kimyen B. Nguyen

    发明人： Desmond Mascarenhas ,  Yang Zhang ,  Pamela S. Olson ,  David R. Olsen ,  Pedro A. Cohen ,  Kimyen B. Nguyen

    IPC分类号： C07K14/47 ,  C07K19/00 ,  C12N15/62

    CPC分类号： C07K14/4743 ,  C07K2319/00 ,  C07K2319/32 ,  C07K2319/35 ,  C07K2319/75 ,  C07K2319/95

    摘要： The invention is directed to the use of a fusion partner that does not contain a leader sequence, as a means to increase the solubility and activity of recombinant polypeptides by facilitating the expression of fusion proteins, which are then transported out of the cytoplasm. The invention includes a nucleic acid encoding a fusion polypeptide comprising a mature interleukin-1-like polypeptide or a leader-deleted-translocating polypeptide, and a polypeptide of interest; as well as host cells comprising such nucleic acids, and fusion proteins so encoded. The invention also encompasses methods of using such nucleic acids to produce recombinant fusion polypeptides, mature polypeptides of interest, and purified compositions thereof.

    摘要翻译： 本发明涉及不含前导序列的融合配偶体的用途，作为通过促进融合蛋白的表达增加重组多肽的溶解度和活性的手段，然后将其转运出细胞质。 本发明包括编码融合多肽的核酸，所述融合多肽包含成熟的白细胞介素-1样多肽或前导缺失的易位多肽和感兴趣的多肽; 以及包含这种核酸的宿主细胞，以及如此编码的融合蛋白。 本发明还包括使用此类核酸来产生重组融合多肽，目标成熟多肽及其纯化组合物的方法。

    Chromosomal expression of heterologous genes in bacterial cells
    6.
    发明授权
    Chromosomal expression of heterologous genes in bacterial cells 失效
    异源基因在细菌细胞中的染色体表达

    公开(公告)号：US5861273A

    公开(公告)日：1999-01-19

    申请号：US482182

    申请日：1995-06-07

    申请人： Pamela S. Olson ,  Desmond Mascarenhas

    发明人： Pamela S. Olson ,  Desmond Mascarenhas

    IPC分类号： C12N15/09 ,  C07K14/395 ,  C07K14/47 ,  C07K14/495 ,  C07K14/65 ,  C12N1/21 ,  C12N9/02 ,  C12N9/16 ,  C12N15/12 ,  C12N15/67 ,  C12N15/70 ,  C12N15/74 ,  C12N15/90 ,  C12P21/02 ,  C12R1/19 ,  C12N15/64

    CPC分类号： C07K14/395 ,  C07K14/4743 ,  C07K14/495 ,  C07K14/65 ,  C12N15/67 ,  C12N15/70 ,  C12N15/74 ,  C12N15/90 ,  C12N9/0036 ,  C12N9/16

    摘要： The present invention provides compositions and methods for producing a heterologous protein of interest by inserting a copy of a gene encoding the heterologous protein of interest into the chromosome of a host cell, such as E. coli. A chromosomal transfer DNA (a circular, non-self-replicating DNA) is used to integrate the gene encoding the heterologous protein of interest into the host cell chromosome. The chromosomal transfer DNA comprises at least one selectable marker and may optionally include repeated DNA sequences flanking the selectable marker, facilitating chromosomal amplification of the integrated DNA. The gene encoding the protein of interest may be expressed after integration into the chromosome of the host cell; selection for chromosomal amplification may be performed prior to expression of the gene.

    摘要翻译： 本发明提供了通过将编码感兴趣的异源蛋白质的基因的拷贝插入宿主细胞如大肠杆菌的染色体来产生感兴趣的异源蛋白质的组合物和方法。 使用染色体转移DNA（圆形，非自我复制DNA）将编码异源目标蛋白的基因整合到宿主细胞染色体中。 染色体转移DNA包含至少一个选择性标记，并且可任选地包括侧翼于选择性标记的重复DNA序列，促进整合DNA的染色体扩增。 编码目标蛋白的基因可以在整合入宿主细胞的染色体后表达; 染色体扩增的选择可以在基因表达之前进行。