利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

46 条记录 (耗时 0.035 秒)

    Expression of fusion polypeptides transported out of the cytoplasm without leader sequences
    5.
    发明授权
    Expression of fusion polypeptides transported out of the cytoplasm without leader sequences 失效
    从细胞质中转移出的融合多肽的表达无前导序列

    公开(公告)号：US5914254A

    公开(公告)日：1999-06-22

    申请号：US854811

    申请日：1997-05-12

    申请人： Desmond Mascarenhas ,  Yang Zhang ,  Pamela S. Olson ,  David R. Olsen ,  Pedro A. Cohen ,  Kimyen B. Nguyen

    发明人： Desmond Mascarenhas ,  Yang Zhang ,  Pamela S. Olson ,  David R. Olsen ,  Pedro A. Cohen ,  Kimyen B. Nguyen

    IPC分类号： C07K14/47 ,  C07K19/00 ,  C12N15/62

    CPC分类号： C07K14/4743 ,  C07K2319/00 ,  C07K2319/32 ,  C07K2319/35 ,  C07K2319/75 ,  C07K2319/95

    摘要： The invention is directed to the use of a fusion partner that does not contain a leader sequence, as a means to increase the solubility and activity of recombinant polypeptides by facilitating the expression of fusion proteins, which are then transported out of the cytoplasm. The invention includes a nucleic acid encoding a fusion polypeptide comprising a mature interleukin-1-like polypeptide or a leader-deleted-translocating polypeptide, and a polypeptide of interest; as well as host cells comprising such nucleic acids, and fusion proteins so encoded. The invention also encompasses methods of using such nucleic acids to produce recombinant fusion polypeptides, mature polypeptides of interest, and purified compositions thereof.

    摘要翻译： 本发明涉及不含前导序列的融合配偶体的用途，作为通过促进融合蛋白的表达增加重组多肽的溶解度和活性的手段，然后将其转运出细胞质。 本发明包括编码融合多肽的核酸，所述融合多肽包含成熟的白细胞介素-1样多肽或前导缺失的易位多肽和感兴趣的多肽; 以及包含这种核酸的宿主细胞，以及如此编码的融合蛋白。 本发明还包括使用此类核酸来产生重组融合多肽，目标成熟多肽及其纯化组合物的方法。

    Chromosomal expression of heterologous genes in bacterial cells
    6.
    发明授权
    Chromosomal expression of heterologous genes in bacterial cells 失效
    异源基因在细菌细胞中的染色体表达

    公开(公告)号：US5861273A

    公开(公告)日：1999-01-19

    申请号：US482182

    申请日：1995-06-07

    申请人： Pamela S. Olson ,  Desmond Mascarenhas

    发明人： Pamela S. Olson ,  Desmond Mascarenhas

    IPC分类号： C12N15/09 ,  C07K14/395 ,  C07K14/47 ,  C07K14/495 ,  C07K14/65 ,  C12N1/21 ,  C12N9/02 ,  C12N9/16 ,  C12N15/12 ,  C12N15/67 ,  C12N15/70 ,  C12N15/74 ,  C12N15/90 ,  C12P21/02 ,  C12R1/19 ,  C12N15/64

    CPC分类号： C07K14/395 ,  C07K14/4743 ,  C07K14/495 ,  C07K14/65 ,  C12N15/67 ,  C12N15/70 ,  C12N15/74 ,  C12N15/90 ,  C12N9/0036 ,  C12N9/16

    摘要： The present invention provides compositions and methods for producing a heterologous protein of interest by inserting a copy of a gene encoding the heterologous protein of interest into the chromosome of a host cell, such as E. coli. A chromosomal transfer DNA (a circular, non-self-replicating DNA) is used to integrate the gene encoding the heterologous protein of interest into the host cell chromosome. The chromosomal transfer DNA comprises at least one selectable marker and may optionally include repeated DNA sequences flanking the selectable marker, facilitating chromosomal amplification of the integrated DNA. The gene encoding the protein of interest may be expressed after integration into the chromosome of the host cell; selection for chromosomal amplification may be performed prior to expression of the gene.

    摘要翻译： 本发明提供了通过将编码感兴趣的异源蛋白质的基因的拷贝插入宿主细胞如大肠杆菌的染色体来产生感兴趣的异源蛋白质的组合物和方法。 使用染色体转移DNA（圆形，非自我复制DNA）将编码异源目标蛋白的基因整合到宿主细胞染色体中。 染色体转移DNA包含至少一个选择性标记，并且可任选地包括侧翼于选择性标记的重复DNA序列，促进整合DNA的染色体扩增。 编码目标蛋白的基因可以在整合入宿主细胞的染色体后表达; 染色体扩增的选择可以在基因表达之前进行。

    MODULATION OF MAMMALIAN CELL LINEAGE BY SYNTHETIC IMMODULINS
    7.
    发明公开
    MODULATION OF MAMMALIAN CELL LINEAGE BY SYNTHETIC IMMODULINS 审中-公开

    公开(公告)号：US20240327480A1

    公开(公告)日：2024-10-03

    申请号：US18264181

    申请日：2021-08-20

    申请人： Desmond Mascarenhas

    发明人： Desmond Mascarenhas

    IPC分类号： C07K14/47 ,  A61K38/00 ,  A61K47/54 ,  A61P35/00 ,  G01N33/50

    CPC分类号： C07K14/4743 ,  A61K47/542 ,  A61P35/00 ,  G01N33/5023 ,  G01N33/5041 ,  A61K38/00 ,  G01N2333/4745

    摘要： This invention provides synthetic immodulin peptides and related compositions and methods. The peptides of this invention exhibit new and surprising biological activities, such as the expansion of specified differentiated mammalian lineages from precursor cell populations which play important roles in post-apoptotic clearance, autoimmunity, targeted vaccination, cancer and trauma, by contacting mammalian cells with the synthetic peptides. Furthermore, the peptides of the invention can be made significantly more potent by each of a series of modifications described in the invention, including a carboxyterminal tripeptide extension to the canonical immodulin core sequence, other peptide extensions with kinase-inhibiting and other domains, substitution with modified amino acids, conjugation to certain bioactive small molecules, complexation to metals or glycosaminoglycans, and co-administration with helper molecules. The invention also teaches compositions and methods for enhancing previously disclosed uses of immodulin peptides to boost the efficacy of immodulin peptides in trauma, immune imbalance, cancer and other medically relevant conditions.

    System and method for using psychological significance pattern information for matching with target information
    8.
    发明授权
    System and method for using psychological significance pattern information for matching with target information 有权
    使用心理意义模式信息与目标信息进行匹配的系统和方法

    公开(公告)号：US08010400B2

    公开(公告)日：2011-08-30

    申请号：US11580665

    申请日：2006-10-13

    申请人： Desmond Mascarenhas

    发明人： Desmond Mascarenhas

    IPC分类号： G06F9/46

    CPC分类号： G06F17/30702 ,  G06F17/30011 ,  G06F17/30707 ,  G06F17/30867 ,  G06F17/30873 ,  G06Q30/02 ,  G06Q30/0201 ,  G06Q30/0203 ,  G06Q30/0204

    摘要： A computer-implemented system for creating a classification significance pattern for end users, and enabling end users to use their classification significance pattern to conduct custom searches for target information, such as information about products, services, and jobs, as well as enabling third parties, such as vendors and potential employers, to target their advertisements to groups of users meeting a certain classification. A classification significance pattern is created by having a user take a psychological test, for example, that includes a personality test, a design taste test, a recreation/travel test, a life satisfaction test, an interactive game module, or a career/job test, and having the system automatically score such test and classifying the user based on a defined abstract classification.

    摘要翻译： 一种用于为最终用户创建分类重要性模式的计算机实现的系统，并且使最终用户能够使用其分类重要性模式来对目标信息进行定制搜索，诸如关于产品，服务和作业的信息，以及使得第三方 ，如供应商和潜在的雇主，将他们的广告定位到满足一定分类的用户群体。 通过使用户进行心理测试来创建分类意义模式，例如包括个性测试，设计味觉测试，娱乐/旅行测试，生活满意度测试，交互式游戏模块或职业/工作 测试，并使系统自动对这样的测试进行评分，并根据定义的抽象分类对用户进行分类。