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    Characterisation of gene function using double stranded RNA inhibition
    2.
    发明申请
    Characterisation of gene function using double stranded RNA inhibition 审中-公开

    公开(公告)号：US20040133943A1

    公开(公告)日：2004-07-08

    申请号：US10738886

    申请日：2003-12-17

    申请人： Devgen NV

    发明人： Geert Plaetinck ,  Christ Platteeuw ,  Katharine Mortier ,  Thierry Bogaert

    IPC分类号： A01H001/00 ,  C12N015/82

    CPC分类号： C12N15/1079 ,  C12N15/1093 ,  C12N15/8216 ,  C12N15/8218 ,  C12N15/8279 ,  C12N15/8285 ,  C12N15/86 ,  C12N2795/10243 ,  C12N2800/40 ,  C12N2830/002 ,  C12N2830/008 ,  C12N2830/42 ,  C12Q1/68 ,  Y02A40/164

    摘要： There is provided a method of identifying DNA responsible for conferring a particular phenotype in a cell which method comprises a) constructing a cDNA or genomic library of the DNA of said cell in a suitable vector in an orientation relative to a promoter(s) capable of initiating transcription of said cDNA or DNA to double stranded (ds) RNA upon binding of an appropriate transcription factor to said promoter(s), b) introducing said library into one or more of said cells comprising said transcription factor, and c) identifying and isolating a particular phenotype of said cell comprising said library and identifying the DNA or cDNA fragment from said library responsible for conferring said phenotype. Using this technique it is also possible to assign function to a known DNA sequence by a) identifying a homologue(s) of said DNA sequence in a cell, b) isolating the relevant DNA homologue(s) or a fragment thereof from said cell, c) cloning said homologue or fragment thereof into an appropriate vector in an orientation relative to a suitable promoter(s) capable of initiating transcription of dsRNA from said DNA homologue or fragment upon binding of an appropriate transcription factor to said promoter(s) and d) introducing said vector into said cell from step a) comprising said transcription factor.

    Characterisation of gene function using double stranded RNA inhibition
    3.
    发明申请
    Characterisation of gene function using double stranded RNA inhibition 有权
    使用双链RNA抑制表征基因功能

    公开(公告)号：US20040187170A1

    公开(公告)日：2004-09-23

    申请号：US10826522

    申请日：2004-04-16

    申请人： Devgen NV

    发明人： Geert Plaetinck ,  Christ Platteeuw ,  Katherine Mortier ,  Thierry Bogaert

    IPC分类号： A01K067/033 ,  C12N015/85

    CPC分类号： C12N15/1079 ,  C12N15/1093 ,  C12N15/8216 ,  C12N15/8218 ,  C12N15/8279 ,  C12N15/8285 ,  C12N15/86 ,  C12N2795/10243 ,  C12N2800/40 ,  C12N2830/002 ,  C12N2830/008 ,  C12N2830/42 ,  C12Q1/68 ,  Y02A40/164

    摘要： There is provided a method of identifying DNA responsible for conferring a particular phenotype in a cell which method comprises a) constructing a cDNA or genomic library of the DNA of said cell in a suitable vector in an orientation relative to a promoter(s) capable of initiating transcription of said cDNA or DNA to double stranded (ds) RNA upon binding of an appropriate transcription factor to said promoter(s), b) introducing said library into one or more of said cells comprising said transcription factor, and c) identifying and isolating a particular phenotype of said cell comprising said library and identifying the DNA or cDNA fragment from said library responsible for conferring said phenotype. Using this technique it is also possible to assign function to a known DNA sequence by a) identifying a homologue(s) of said DNA sequence in a cell, b) isolating the relevant DNA homologue(s) or a fragment thereof from said cell, c) cloning said homologue or fragment thereof into an appropriate vector in an orientation relative to a suitable promoter(s) capable of initiating transcription of dsRNA from said DNA homologue or fragment upon binding of an appropriate transcription factor to said promoter(s) and d) introducing said vector into said cell from step a) comprising said transcription factor.

    摘要翻译： 提供了鉴定负责赋予细胞中特定表型的DNA的方法，该方法包括a）以合适的载体构建所述细胞的DNA的cDNA或基因组文库，其方向相对于能够 在将合适的转录因子结合到所述启动子时，将所述cDNA或DNA转录到双链（ds）RNA; b）将所述文库引入到一个或多个包含所述转录因子的细胞中，和c）鉴定和 分离包含所述文库的所述细胞的特定表型，并从负责赋予所述表型的所述文库鉴定DNA或cDNA片段。 使用这种技术，还可以通过以下方式将功能分配给已知的DNA序列：a）鉴定细胞中所述DNA序列的同源物，b）从所述细胞中分离相关的DNA同系物或其片段， c）相对于合适的启动子，将所述同源物或片段相对于适当的启动子克隆到适当的载体中，所述启动子能够在合适的转录因子与所述启动子结合时从所述DNA同源物或片段启动dsRNA的转录，并且d ）将所述载体从包含所述转录因子的步骤a）引入所述细胞。

    Compound assay
    5.
    发明申请
    Compound assay 失效
    化合物检测

    公开(公告)号：US20040034880A1

    公开(公告)日：2004-02-19

    申请号：US10638670

    申请日：2003-08-11

    申请人： Devgen NV

    发明人： Philippe Verwaerde ,  Richard Feichtinger ,  Myriam Behgyn ,  Thierry Bogaert

    IPC分类号： A01K067/033

    CPC分类号： G01N33/502 ,  A01K67/0336 ,  A01K2227/703 ,  G01N33/5008 ,  G01N33/5014 ,  G01N33/5058 ,  G01N33/5085 ,  G01N33/5091 ,  G01N2333/43534

    摘要： The invention provides methods of screening compounds for potential pharmacological activity using nematode worms, principally but not exclusively the nematode C. elegans. Specifically, the invention relates to the use of nematodes modified to have certain characteristics which provide advantages for compound screening, such as constitutive pharyngeal pumping, increased gut permeability or altered gut molecular transport. Methods for selecting suitably modified nematodes from a population of nematodes are also provided.

    摘要翻译： 本发明提供了使用线虫，主要但不仅限于线虫线虫筛选化合物潜在药理活性的方法。 具体地，本发明涉及经修饰的线虫具有某些特征的用途，这些特征为复合筛选提供了优势，例如组成型咽喉抽吸，肠道渗透性增加或肠道分子转运增加。 还提供了从线虫种群中选择适当修饰的线虫的方法。