公开(公告)号：US20170044559A1

公开(公告)日：2017-02-16

申请号：US15292774

申请日：2016-10-13

申请人： Dow AgroSciences LLC ,  Sangamo BioSciences, Inc.

发明人： W. Michael Ainley ,  Steven R. Webb ,  Jayakumar P. Samuel ,  Dmitry Y. Guschin ,  Jeffrey C. Miller ,  Lei Zhang

IPC分类号： C12N15/82 ,  C07K14/47 ,  C12N9/22

CPC分类号： C12N15/8216 ,  C07K14/4702 ,  C07K2319/81 ,  C12N9/22 ,  C12N15/102 ,  C12N15/66 ,  C12N15/822 ,  C12N15/8247 ,  C12N15/902 ,  C12Y301/00

摘要： Methods and compositions for gene disruption, gene editing or gene stacking within a FAD2 loci by cleaving, in a site directed manner, a location in a FAD2 gene in a soybean cell, to generate a break in the FAD2 gene and then optionally integrating into the break a nucleic acid molecule of interest is disclosed.

摘要翻译： FAD2基因座中的基因破坏，基因编辑或基因堆叠的方法和组合，通过以位点方式切割大豆细胞中FAD2基因的位置，在FAD2基因中产生断裂，然后任选地整合入 揭示目的核酸分子。

公开(公告)号：US10174331B2

公开(公告)日：2019-01-08

申请号：US13889162

申请日：2013-05-07

申请人： Sangamo BioSciences, Inc. ,  Dow AgroSciences LLC

发明人： Gregory J. Cost ,  Fyodor Urnov ,  W. Michael Ainley ,  Joseph F. Petolino ,  Jayakumar Pon Samuel ,  Steven R. Webb ,  Lakshmi Sastry-Dent

IPC分类号： C12N15/82 ,  C12N15/87 ,  C12N15/85 ,  C12N15/10

摘要： Disclosed herein are methods and compositions for homology-independent targeted insertion of donor molecules into the genome of a cell.

公开(公告)号：US20140304853A1

公开(公告)日：2014-10-09

申请号：US14243553

申请日：2014-04-02

申请人： Sangamo BioSciences, Inc. ,  Dow AgroSciences LLC

发明人： W. Michael Ainley ,  Dmitry Y. Guschin ,  Matthew Hayden ,  Daniel Isenegger ,  John Mason ,  Jeffrey C. Miller ,  Joseph F. Petolino ,  Yidong Ran ,  Tim Sawbridge ,  German Spangenberg ,  Steven R. Webb

IPC分类号： C12N15/82

CPC分类号： C12N15/8213 ,  C12N15/8274 ,  C12N15/8278 ,  C12N15/90 ,  C12N2800/80

摘要： Disclosed herein are methods and compositions for parallel or sequential transgene stacking in plants to produce plants with selected phenotypes.

摘要翻译： 本文公开了用于在植物中平行或顺序转基因堆叠以产生具有选定表型的植物的方法和组合物。

公开(公告)号：US20200071712A1

公开(公告)日：2020-03-05

申请号：US16674831

申请日：2019-11-05

申请人： Dow AgroSciences LLC ,  Sangamo Therapeutics, Inc.

发明人： W. Michael Ainley ,  Dmitry Y. Guschin ,  Matthew Hayden ,  Daniel Isenegger ,  John Mason ,  Jeffrey C. Miller ,  Joseph F. Petolino ,  Yidong Ran ,  Tim Sawbridge ,  German Spangenberg ,  Steven R. Webb

IPC分类号： C12N15/82 ,  C12N15/90

摘要： Disclosed herein are methods and compositions for parallel or sequential transgene stacking in plants to produce plants with selected phenotypes.

公开(公告)号：US10577616B2

公开(公告)日：2020-03-03

申请号：US15943106

申请日：2018-04-02

申请人： Dow AgroSciences LLC ,  Sangamo Therapeutics, Inc.

发明人： Noel Cogan ,  John Forster ,  Matthew Hayden ,  Tim Sawbridge ,  German Spangenberg ,  Steven R. Webb ,  Manju Gupta ,  W. Michael Ainley ,  Matthew J. Henry ,  Jeffrey C. Miller ,  Dmitry Y. Guschin

IPC分类号： C12N15/82 ,  C12N15/90

摘要： A method of gene editing or gene stacking within a FAD2 loci by cleaving, in a site directed manner, a location in a FAD2 gene in a cell, to generate a break in the FAD2 gene and then ligating into the break a nucleic acid molecule associated with one or more traits of interest is disclosed.

公开(公告)号：US20200087671A1

公开(公告)日：2020-03-19

申请号：US16698077

申请日：2019-11-27

申请人： Dow AgroSciences LLC ,  Sangamo Therapeutics, Inc.

发明人： Noel Cogan ,  John Forster ,  Matthew Hayden ,  Tim Sawbridge ,  German Spangenberg ,  Steven R. Webb ,  Manju Gupta ,  W. Michael Ainley ,  Matthew J. Henry ,  Jeffrey C. Miller ,  Dmitry Y. Guschin

IPC分类号： C12N15/79 ,  C12N15/82 ,  C12N15/63 ,  C12N9/22

摘要： A method of gene editing or gene stacking within a FAD3 loci by cleaving, in a site directed manner, a location in a FAD3 gene in a cell, to generate a break in the FAD3 gene and then ligating into the break a nucleic acid molecule associated with one or more traits of interest is disclosed.

公开(公告)号：US20180371478A1

公开(公告)日：2018-12-27

申请号：US16123326

申请日：2018-09-06

申请人： DOW AGROSCIENCES LLC

发明人： Lakshmi Sastry-Dent ,  Zehui Cao ,  Shreedharan Sriram ,  Steven R. Webb ,  Debra L. Camper ,  W. Michael Ainley

IPC分类号： C12N15/82 ,  A01H5/10

摘要： As disclosed herein, optimal native genomic loci of soybean plants have been identified that represent best sites for targeted insertion of exogenous sequences.

公开(公告)号：US10160975B2

公开(公告)日：2018-12-25

申请号：US14458086

申请日：2014-08-12

申请人： Dow AgroSciences LLC

发明人： W. Michael Ainley ,  Ryan C. Blue ,  Michael G. Murray ,  David Richard Corbin ,  Rebecca Ruth Miles ,  Steven R. Webb

IPC分类号： C12N15/82

摘要： A method for producing a transgenic plant includes providing a nucleic acid molecule comprising at least two regions of nucleic acid sequence that lack sequence homology with genomic DNA of the plant cell, and at least two zinc finger nuclease recognition sites, wherein the at least two regions of nucleic acid sequence that lack sequence homology with genomic DNA of the plant cell flank the at least two zinc finger nuclease recognition sites. A plant cell or tissue having the nucleic acid molecule stably integrated into the genome of the plant cell is transformed. A plant is regenerated from the plant cell. Transgenic plants are produced by the method. Seeds are produced by the transgenic plants.

公开(公告)号：US10961540B2

公开(公告)日：2021-03-30

申请号：US16698077

申请日：2019-11-27

申请人： Dow AgroSciences LLC ,  Sangamo Therapeutics, Inc.

发明人： Noel Cogan ,  John Forster ,  Matthew Hayden ,  Tim Sawbridge ,  German Spangenberg ,  Steven R. Webb ,  Manju Gupta ,  W. Michael Ainley ,  Matthew J. Henry ,  Jeffrey C. Miller ,  Dmitry Y. Guschin

IPC分类号： C12N15/82 ,  C12N9/22 ,  C12N15/63 ,  C12N15/79

摘要： A method of gene editing or gene stacking within a FAD3 loci by cleaving, in a site directed manner, a location in a FAD3 gene in a cell, to generate a break in the FAD3 gene and then ligating into the break a nucleic acid molecule associated with one or more traits of interest is disclosed.

公开(公告)号：US10415046B2

公开(公告)日：2019-09-17

申请号：US15338888

申请日：2016-10-31

申请人： DOW AGROSCIENCES LLC

发明人： W. Michael Ainley ,  James W. Bing ,  David R. Corbin ,  Steven L. Evans ,  Joseph F. Petolino ,  Lakshmi Sastry-Dent ,  Steven A. Thompson ,  Steven R. Webb ,  Mary E. Welter ,  Ning Zhou

IPC分类号： C12N15/82 ,  C12N9/12

摘要： The present invention claims methods for the stable integration of exogenous DNA into a specific locus, E32, in the maize genome through the use of zinc finger nucleases. Maize plants and plant parts that were transformed by the methods of the invention are claimed. The invention is useful for creating desirable traits such as herbicide resistance, herbicide tolerance, insect resistance, insect tolerance, disease resistance, disease tolerance, stress tolerance, and stress resistance in maize The E32 locus represents a superior site for inserting foreign genes because native agronomic phenotypes are not disturbed.