公开(公告)号：US20070243565A1

公开(公告)日：2007-10-18

申请号：US11752596

申请日：2007-05-23

申请人： Dudley Williams ,  Marcella Gilmore ,  Lance Steward ,  Marc Verhagen ,  Kei Aoki ,  Ester Fernandez-Salas

发明人： Dudley Williams ,  Marcella Gilmore ,  Lance Steward ,  Marc Verhagen ,  Kei Aoki ,  Ester Fernandez-Salas

IPC分类号： G01N33/554 ,  C07H19/00 ,  C12N1/20 ,  C12N15/00

CPC分类号： C12Q1/37 ,  C07K14/001 ,  C07K14/435 ,  G01N33/542 ,  G01N33/56911 ,  G01N2333/33

摘要： Clostridial toxin substrates comprising a lanthanide donor complex, an acceptor, and a Clostridial toxin recognition sequence including a cleavage site; methods for determining the activity of a Clostridial toxin from a test sample using such Clostridial toxin substrates; cell compositions comprising such Clostridial toxin substrates and a Clostridial toxin receptor; and methods for determining the activity of a Clostridial toxin from a test sample using such cell compositions.

摘要翻译： 包含镧系元素供体复合物，受体和包含切割位点的梭菌毒素识别序列的梭菌毒素底物; 使用这种梭菌毒素底物从测试样品测定梭菌毒素的活性的方法; 包含这种梭菌毒素底物和梭菌毒素受体的细胞组合物; 以及使用这种细胞组合物从测试样品测定梭菌毒素的活性的方法。

公开(公告)号：US20060063221A1

公开(公告)日：2006-03-23

申请号：US10947071

申请日：2004-09-21

申请人： Dudley Williams ,  Marcella Gilmore ,  Lance Steward ,  Marc Verhagen ,  Kei Aoki

发明人： Dudley Williams ,  Marcella Gilmore ,  Lance Steward ,  Marc Verhagen ,  Kei Aoki

IPC分类号： C12Q1/37 ,  C12N11/16

CPC分类号： G01N33/56911 ,  C12Q1/37 ,  G01N2333/33 ,  G01N2458/40

摘要： The present invention provides a clostridial toxin substrate that contains (a) a lanthanide donor complex; (b) an acceptor having an absorbance spectrum overlapping the emission spectrum of the lanthanide donor complex; and (c) a clostridial toxin recognition sequence containing a cleavage site that intervenes between the lanthanide donor complex and the acceptor, where, under the appropriate conditions, resonance energy transfer is exhibited between the lanthanide donor complex and the acceptor.

摘要翻译： 本发明提供了一种梭菌毒素底物，其含有（a）镧系元素供体复合物; （b）吸收光谱与镧系元素供体络合物的发射光谱重叠的受体; 和（c）含有介于镧系元素供体复合物和受体之间的切割位点的梭菌毒素识别序列，其中在合适的条件下，在镧系元素供体复合物和受体之间显示共振能量转移。

公开(公告)号：US20060063222A1

公开(公告)日：2006-03-23

申请号：US10948097

申请日：2004-09-22

申请人： Dudley Williams ,  Marcella Gilmore ,  Lance Steward ,  Marc Verhagen ,  Kei Aoki

发明人： Dudley Williams ,  Marcella Gilmore ,  Lance Steward ,  Marc Verhagen ,  Kei Aoki

IPC分类号： C12Q1/37 ,  C12N11/16

CPC分类号： G01N33/542 ,  C12N9/52 ,  C12Q1/37 ,  G01N2333/33

摘要： The present invention provides a method of determining the presence or activity of a clostridial toxin by (a) treating with a sample, under conditions suitable for clostridial toxin protease activity, a clostridial toxin substrate which includes a fluorophore; a bulking group; and a clostridial toxin recognition sequence containing a cleavage site that intervenes between the fluorophore and the bulking group; (b) exciting the fluorophore with plane polarized light; and (c) determining fluorescence polarization of the treated substrate relative to a control substrate, where a change in fluorescence polarization of the treated substrate as compared to fluorescence polarization of the control substrate is indicative of the presence or activity of the clostridial toxin.

公开(公告)号：US20070275477A1

公开(公告)日：2007-11-29

申请号：US11548411

申请日：2006-10-11

申请人： Marcella Gilmore ,  Dudley Williams ,  Lance Steward ,  Kei Aoki

发明人： Marcella Gilmore ,  Dudley Williams ,  Lance Steward ,  Kei Aoki

IPC分类号： G01N21/76 ,  G01N33/68

CPC分类号： G01N33/542

摘要： The present invention provides a method of determining the proximity, or changes in the proximity of two or more molecular features labeled with fluorophores able to undergo DARET. In preferred embodiments the change in proximity of molecular features in a sample is correlated with changes in the fluorescence polarization of the sample, and may be monitored in real time.

摘要翻译： 本发明提供了一种确定两个或多个分子特征的邻近性的变化或方法，所述分子特征用能够进行DARET的荧光团标记。 在优选实施例中，样品中分子特征的接近度的变化与样品的荧光偏振的变化相关，并且可以实时监测。

公开(公告)号：US20080020657A1

公开(公告)日：2008-01-24

申请号：US11579032

申请日：2005-04-29

申请人： Dudley Williams

发明人： Dudley Williams

IPC分类号： B63H5/16

CPC分类号： B63H20/36

摘要： Apparatus (10) for protecting the out-drive (13) of a watercraft (11) comprises a locating member (21) adapted for attachment to the underside of the marlin board (17) of the watercraft (10) and a shroud (23) engageable with the locating member (21) to provide an enclosure about the out-drive (13). The shroud (23) is buoyant and is adapted to be floated into sliding engagement with the locating member (21). The shroud (23) has an opening (40) which is closed upon engagement of the shroud (23) with the transom (15) of the watercraft (11) to prevent ingress of water into the interior of the shroud (23). A connection means (180) and the locking means (80) are provided for releasably connecting the shroud (23) to the locating member (21).

摘要翻译： 用于保护船舶（11）的驱动器（13）的装置（10）包括适于附接到船只（10）的马林板（17）的下侧的定位构件（21）和护罩（23） ）可与所述定位构件（21）接合以提供围绕所述外驱动器（13）的外壳。 护罩（23）是浮力的并且适于浮动地与定位构件（21）滑动接合。 护罩（23）具有开口（40），该开口（40）在护罩（23）与船只（11）的横梁（15）接合时关闭，以防止水进入护罩（23）的内部。 设置连接装置（180）和锁定装置（80）以将护罩（23）可释放地连接到定位构件（21）。