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1.发明申请LUMINESCENCE RESONANCE ENERGY TRANSFER (LRET) ASSAYS FOR CLOSTRIDIAL TOXIN ACTIVITY 有权光合能力转移（LRET）用于离子毒素活性的测定公开(公告)号：US20070243565A1
公开(公告)日：2007-10-18
申请号：US11752596
申请日：2007-05-23
申请人： Dudley Williams , Marcella Gilmore , Lance Steward , Marc Verhagen , Kei Aoki , Ester Fernandez-Salas
发明人： Dudley Williams , Marcella Gilmore , Lance Steward , Marc Verhagen , Kei Aoki , Ester Fernandez-Salas
IPC分类号： G01N33/554 , C07H19/00 , C12N1/20 , C12N15/00
CPC分类号： C12Q1/37 , C07K14/001 , C07K14/435 , G01N33/542 , G01N33/56911 , G01N2333/33
摘要： Clostridial toxin substrates comprising a lanthanide donor complex, an acceptor, and a Clostridial toxin recognition sequence including a cleavage site; methods for determining the activity of a Clostridial toxin from a test sample using such Clostridial toxin substrates; cell compositions comprising such Clostridial toxin substrates and a Clostridial toxin receptor; and methods for determining the activity of a Clostridial toxin from a test sample using such cell compositions.
摘要翻译： 包含镧系元素供体复合物，受体和包含切割位点的梭菌毒素识别序列的梭菌毒素底物; 使用这种梭菌毒素底物从测试样品测定梭菌毒素的活性的方法; 包含这种梭菌毒素底物和梭菌毒素受体的细胞组合物; 以及使用这种细胞组合物从测试样品测定梭菌毒素的活性的方法。
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公开(公告)号：US20060063222A1
公开(公告)日：2006-03-23
申请号：US10948097
申请日：2004-09-22
申请人： Dudley Williams , Marcella Gilmore , Lance Steward , Marc Verhagen , Kei Aoki
发明人： Dudley Williams , Marcella Gilmore , Lance Steward , Marc Verhagen , Kei Aoki
CPC分类号： G01N33/542 , C12N9/52 , C12Q1/37 , G01N2333/33
摘要： The present invention provides a method of determining the presence or activity of a clostridial toxin by (a) treating with a sample, under conditions suitable for clostridial toxin protease activity, a clostridial toxin substrate which includes a fluorophore; a bulking group; and a clostridial toxin recognition sequence containing a cleavage site that intervenes between the fluorophore and the bulking group; (b) exciting the fluorophore with plane polarized light; and (c) determining fluorescence polarization of the treated substrate relative to a control substrate, where a change in fluorescence polarization of the treated substrate as compared to fluorescence polarization of the control substrate is indicative of the presence or activity of the clostridial toxin.
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3.发明申请Lanthanide-based substrates and methods for determining clostridial toxin activity 审中-公开基于镧系元素的底物和确定梭菌毒素活性的方法公开(公告)号：US20060063221A1
公开(公告)日：2006-03-23
申请号：US10947071
申请日：2004-09-21
申请人： Dudley Williams , Marcella Gilmore , Lance Steward , Marc Verhagen , Kei Aoki
发明人： Dudley Williams , Marcella Gilmore , Lance Steward , Marc Verhagen , Kei Aoki
CPC分类号： G01N33/56911 , C12Q1/37 , G01N2333/33 , G01N2458/40
摘要： The present invention provides a clostridial toxin substrate that contains (a) a lanthanide donor complex; (b) an acceptor having an absorbance spectrum overlapping the emission spectrum of the lanthanide donor complex; and (c) a clostridial toxin recognition sequence containing a cleavage site that intervenes between the lanthanide donor complex and the acceptor, where, under the appropriate conditions, resonance energy transfer is exhibited between the lanthanide donor complex and the acceptor.
摘要翻译： 本发明提供了一种梭菌毒素底物，其含有（a）镧系元素供体复合物; （b）吸收光谱与镧系元素供体络合物的发射光谱重叠的受体; 和（c）含有介于镧系元素供体复合物和受体之间的切割位点的梭菌毒素识别序列，其中在合适的条件下，在镧系元素供体复合物和受体之间显示共振能量转移。
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4.发明授权公开(公告)号：US07399607B2
公开(公告)日：2008-07-15
申请号：US10948097
申请日：2004-09-22
CPC分类号： G01N33/542 , C12N9/52 , C12Q1/37 , G01N2333/33
摘要： The present invention provides a method of determining the presence or activity of a clostridial toxin by (a) treating with a sample, under conditions suitable for clostridial toxin protease activity, a clostridial toxin substrate which includes a fluorophore; a bulking group; and a clostridial toxin recognition sequence containing a cleavage site that intervenes between the fluorophore and the bulking group; (b) exciting the fluorophore with plane polarized light; and (c) determining fluorescence polarization of the treated substrate relative to a control substrate, where a change in fluorescence polarization of the treated substrate as compared to fluorescence polarization of the control substrate is indicative of the presence or activity of the clostridial toxin.
摘要翻译： 本发明提供一种通过（a）在适合于梭菌毒素蛋白酶活性的条件下用样品处理包括荧光团的梭菌毒素底物来确定梭菌毒素的存在或活性的方法; 一个胖胖的小组 和含有插入在荧光团和膨化基团之间的切割位点的梭菌毒素识别序列; （b）用平面偏振光激发荧光团; 和（c）确定经处理的底物相对于对照底物的荧光偏振，其中与对照底物的荧光偏振相比，经处理的底物的荧光偏振的变化指示梭菌毒素的存在或活性。
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公开(公告)号：US07632655B2
公开(公告)日：2009-12-15
申请号：US12125723
申请日：2008-05-22
CPC分类号： G01N33/542 , C12N9/52 , C12Q1/37 , G01N2333/33
摘要： The present invention provides a method of determining the presence or activity of a clostridial toxin by (a) treating with a sample, under conditions suitable for clostridial toxin protease activity, a clostridial toxin substrate which includes a fluorophore; a bulking group; and a clostridial toxin recognition sequence containing a cleavage site that intervenes between the fluorophore and the bulking group; (b) exciting the fluorophore with plane polarized light; and (c) determining fluorescence polarization of the treated substrate relative to a control substrate, where a change in fluorescence polarization of the treated substrate as compared to fluorescence polarization of the control substrate is indicative of the presence or activity of the clostridial toxin.
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6.发明授权公开(公告)号：US07674601B2
公开(公告)日：2010-03-09
申请号：US12125327
申请日：2008-05-22
CPC分类号： G01N33/542 , C12N9/52 , C12Q1/37 , G01N2333/33
摘要： The present invention provides a method of determining the presence or activity of a clostridial toxin by (a) treating with a sample, under conditions suitable for clostridial toxin protease activity, a clostridial toxin substrate which includes a fluorophore; a bulking group; and a clostridial toxin recognition sequence containing a cleavage site that intervenes between the fluorophore and the bulking group; (b) exciting the fluorophore with plane polarized light; and (c) determining fluorescence polarization of the treated substrate relative to a control substrate, where a change in fluorescence polarization of the treated substrate as compared to fluorescence polarization of the control substrate is indicative of the presence or activity of the clostridial toxin.
摘要翻译： 本发明提供一种通过（a）在适合于梭菌毒素蛋白酶活性的条件下用样品处理包括荧光团的梭菌毒素底物来确定梭菌毒素的存在或活性的方法; 一个胖胖的小组 和含有插入在荧光团和膨化基团之间的切割位点的梭菌毒素识别序列; （b）用平面偏振光激发荧光团; 和（c）确定经处理的底物相对于对照底物的荧光偏振，其中与对照底物的荧光偏振相比，经处理的底物的荧光偏振的变化指示梭菌毒素的存在或活性。
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7.发明授权公开(公告)号：US07638294B2
公开(公告)日：2009-12-29
申请号：US12124412
申请日：2008-05-21
CPC分类号： G01N33/542 , C12N9/52 , C12Q1/37 , G01N2333/33
摘要： The present invention provides a method of determining the presence or activity of a clostridial toxin by (a) treating with a sample, under conditions suitable for clostridial toxin protease activity, a clostridial toxin substrate which includes a fluorophore; a bulking group; and a clostridial toxin recognition sequence containing a cleavage site that intervenes between the fluorophore and the bulking group; (b) exciting the fluorophore with plane polarized light; and (c) determining fluorescence polarization of the treated substrate relative to a control substrate, where a change in fluorescence polarization of the treated substrate as compared to fluorescence polarization of the control substrate is indicative of the presence or activity of the clostridial toxin.
摘要翻译： 本发明提供一种通过（a）在适合于梭菌毒素蛋白酶活性的条件下用样品处理包括荧光团的梭菌毒素底物来确定梭菌毒素的存在或活性的方法; 一个胖胖的小组 和含有插入在荧光团和膨化基团之间的切割位点的梭菌毒素识别序列; （b）用平面偏振光激发荧光团; 和（c）确定经处理的底物相对于对照底物的荧光偏振，其中与对照底物的荧光偏振相比，经处理的底物的荧光偏振的变化指示梭菌毒素的存在或活性。
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公开(公告)号：US20080305509A1
公开(公告)日：2008-12-11
申请号：US12124412
申请日：2008-05-21
IPC分类号： C12Q1/37
CPC分类号： G01N33/542 , C12N9/52 , C12Q1/37 , G01N2333/33
摘要： The present invention provides a method of determining the presence or activity of a clostridial toxin by (a) treating with a sample, under conditions suitable for clostridial toxin protease activity, a clostridial toxin substrate which includes a fluorophore; a bulking group; and a clostridial toxin recognition sequence containing a cleavage site that intervenes between the fluorophore and the bulking group; (b) exciting the fluorophore with plane polarized light; and (c) determining fluorescence polarization of the treated substrate relative to a control substrate, where a change in fluorescence polarization of the treated substrate as compared to fluorescence polarization of the control substrate is indicative of the presence or activity of the clostridial toxin.
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公开(公告)号：US20080305510A1
公开(公告)日：2008-12-11
申请号：US12125723
申请日：2008-05-22
IPC分类号： C12Q1/37
CPC分类号： G01N33/542 , C12N9/52 , C12Q1/37 , G01N2333/33
摘要： The present invention provides a method of determining the presence or activity of a clostridial toxin by (a) treating with a sample, under conditions suitable for clostridial toxin protease activity, a clostridial toxin substrate which includes a fluorophore; a bulking group; and a clostridial toxin recognition sequence containing a cleavage site that intervenes between the fluorophore and the bulking group; (b) exciting the fluorophore with plane polarized light; and (c) determining fluorescence polarization of the treated substrate relative to a control substrate, where a change in fluorescence polarization of the treated substrate as compared to fluorescence polarization of the control substrate is indicative of the presence or activity of the clostridial toxin.
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公开(公告)号：US20080293085A1
公开(公告)日：2008-11-27
申请号：US12125327
申请日：2008-05-22
IPC分类号： C12Q1/37
CPC分类号： G01N33/542 , C12N9/52 , C12Q1/37 , G01N2333/33
摘要： The present invention provides a method of determining the presence or activity of a clostridial toxin by (a) treating with a sample, under conditions suitable for clostridial toxin protease activity, a clostridial toxin substrate which includes a fluorophore; a bulking group; and a clostridial toxin recognition sequence containing a cleavage site that intervenes between the fluorophore and the bulking group; (b) exciting the fluorophore with plane polarized light; and (c) determining fluorescence polarization of the treated substrate relative to a control substrate, where a change in fluorescence polarization of the treated substrate as compared to fluorescence polarization of the control substrate is indicative of the presence or activity of the clostridial toxin.
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