公开(公告)号：US20190015520A1

公开(公告)日：2019-01-17

申请号：US16064424

申请日：2016-12-21

Applicant: Duke University

Inventor： Ashutosh Chilkoti ,  Yizhi Qi ,  Michael S. Hershfield ,  Nancy J. Ganson

IPC: A61K47/64 ,  A61K47/60 ,  A61K47/65 ,  C12N9/06

Abstract: Disclosed herein are compositions and methods for reducing the antigenicity of molecules. The antigenicity of a molecule may be reduced or eliminated by conjugating at least one branched polymer to the molecule to form a molecule-polymer conjugate. The branched polymer may include a backbone and a plurality of side chains, each side chain covalently attached to the backbone.

公开(公告)号：US10364451B2

公开(公告)日：2019-07-30

申请号：US15387536

申请日：2016-12-21

Applicant: Duke University

Inventor： Ashutosh Chilkoti ,  Yizhi Qi ,  Michael S. Hershfield ,  Nancy J. Ganson

IPC: C12P21/00 ,  C12N9/96 ,  A61K47/60 ,  A61K47/58

Abstract: Disclosed herein are compositions and methods for reducing the antigenicity of molecules. The antigenicity of a molecule may be reduced or eliminated by conjugating at least one branched polymer to the molecule to form a molecule-polymer conjugate. The branched polymer may include a backbone and a plurality of side chains, each side chain covalently attached to the backbone.

公开(公告)号：US20170239363A1

公开(公告)日：2017-08-24

申请号：US15387536

申请日：2016-12-21

Applicant: Duke University

Inventor： Ashutosh Chilkoti ,  Yizhi Qi ,  Michael S. Hershfield ,  Nancy J. Ganson

IPC: A61K47/48 ,  C07K1/107 ,  C12P21/00 ,  C08F265/04 ,  C12N9/96

CPC classification number: C12P21/00 ,  A61K47/58 ,  A61K47/60 ,  C08F2438/00 ,  C12N9/96 ,  C12P21/06

Abstract: Disclosed herein are compositions and methods for reducing the antigenicity of molecules. The antigenicity of a molecule may be reduced or eliminated by conjugating at least one branched polymer to the molecule to form a molecule-polymer conjugate. The branched polymer may include a backbone and a plurality of side chains, each side chain covalently attached to the backbone.

公开(公告)号：US20210046188A1

公开(公告)日：2021-02-18

申请号：US17015315

申请日：2020-09-09

Applicant: Duke University

Inventor： Angus Hucknall ,  Ashutosh Chilkoti ,  Daniel Joh ,  Nancy J. Ganson ,  Yizhi Qi ,  Michael S. Hershfield

IPC: A61K47/58 ,  A61K47/59 ,  A61K47/64 ,  C08L71/08

Abstract: Disclosed herein are compositions and methods for reducing the antigenicity of molecules. The antigenicity of a molecule may be reduced or eliminated by conjugating at least one branched polymer to the molecule to form a molecule-polymer conjugate. The branched polymer may include a backbone and a plurality of side chains, each side chain covalently attached to the backbone.

公开(公告)号：US20180369399A1

公开(公告)日：2018-12-27

申请号：US16064425

申请日：2016-12-21

Applicant: Duke University

Inventor： Michael S. Hershfield ,  Nancy J. Ganson ,  Ashutosh Chilkoti ,  Yizhi Qi

IPC: A61K47/60 ,  C08L71/08 ,  A61K47/65 ,  C12N9/06

Abstract: Disclosed herein are compositions and methods for reducing the antigenicity of molecules, wherein the molecule comprises a uricase. The antigenicity of a molecule may be reduced or eliminated by conjugating at least one branched polymer to the molecule to form a molecule-polymer conjugate. The branched polymer may include a backbone and a plurality of side chains, each side chain covalently attached to the backbone.

公开(公告)号：US11752213B2

公开(公告)日：2023-09-12

申请号：US17015315

申请日：2020-09-09

Applicant: Duke University

Inventor： Angus Hucknall ,  Ashutosh Chilkoti ,  Daniel Joh ,  Nancy J. Ganson ,  Yizhi Qi ,  Michael S. Hershfield

IPC: A61K47/58 ,  A61K47/59 ,  C08L71/08 ,  A61K47/64

CPC classification number: A61K47/58 ,  A61K47/59 ,  A61K47/641 ,  C08L71/08 ,  C08L2203/02

Abstract: Disclosed herein are compositions and methods for reducing the antigenicity of molecules. The antigenicity of a molecule may be reduced or eliminated by conjugating at least one branched polymer to the molecule to form a molecule-polymer conjugate. The branched polymer may include a backbone and a plurality of side chains, each side chain covalently attached to the backbone.

公开(公告)号：US10392611B2

公开(公告)日：2019-08-27

申请号：US15387540

申请日：2016-12-21

Applicant: Duke University

Inventor： Ashutosh Chilkoti ,  Yizhi Qi ,  Michael S. Hershfield ,  Nancy J. Ganson

IPC: C12N9/96 ,  A61K38/44 ,  C12N9/06 ,  A61K47/60 ,  A61K47/58 ,  C08G81/00 ,  C08F265/10 ,  C08F220/28

Abstract: Disclosed herein are compositions and methods for reducing the antigenicity of molecules, wherein the molecule comprises a uricase. The antigenicity of a molecule may be reduced or eliminated by conjugating at least one branched polymer to the molecule to form a molecule-polymer conjugate. The branched polymer may include a backbone and a plurality of side chains, each side chain covalently attached to the backbone.

公开(公告)号：US20170233714A1

公开(公告)日：2017-08-17

申请号：US15387540

申请日：2016-12-21

Applicant: Duke University

Inventor： Ashutosh Chilkoti ,  Yizhi Qi ,  Michael S. Hershfield ,  Nancy J. Ganson

IPC: C12N9/96 ,  C08F289/00 ,  C12N9/06 ,  A61K38/44

CPC classification number: C12N9/96 ,  A61K38/44 ,  A61K47/58 ,  A61K47/60 ,  C08F265/10 ,  C08F2220/286 ,  C08F2438/01 ,  C08F2438/03 ,  C08G81/00 ,  C12N9/0046 ,  C12Y107/03

Abstract: Disclosed herein are compositions and methods for reducing the antigenicity of molecules, wherein the molecule comprises a uricase. The antigenicity of a molecule may be reduced or eliminated by conjugating at least one branched polymer to the molecule to form a molecule-polymer conjugate. The branched polymer may include a backbone and a plurality of side chains, each side chain covalently attached to the backbone.

公开(公告)号：US20180223263A1

公开(公告)日：2018-08-09

申请号：US15679401

申请日：2017-08-17

Applicant: HORIZON PHARMA RHEUMATOLOGY LLC ,  DUKE UNIVERSITY

Inventor： Merry R. Sherman ,  Mark G. P Saifer ,  L. David Williams ,  Michael S. Hershfield ,  Susan J. Kelly

IPC: C12N9/06 ,  A61K47/60 ,  C12N9/02 ,  C12N9/96

CPC classification number: C12N9/0048 ,  A61K38/00 ,  A61K47/60 ,  C12N9/0046 ,  C12N9/0093 ,  C12N9/96 ,  C12Y107/03003

Abstract: A naturally occurring or recombinant protein, especially a mutein of porcine urate oxidase (uricase), that is essentially free of large aggregates can be rendered substantially non-immunogenic by conjugation with a sufficiently small number of strands of polymer such that the bioactivity of the protein is essentially retained in the conjugate. Such conjugates are unusually well suited for treatment of chronic conditions because they are less likely to induce the formation of antibodies and/or accelerated clearance than are similar conjugates prepared from protein preparations containing traces of large aggregates.

公开(公告)号：US09885024B2

公开(公告)日：2018-02-06

申请号：US14806494

申请日：2015-07-22

Applicant: Duke University ,  Crealta Pharmaceuticals LLC

Inventor： L. David Williams ,  Michael S. Hershfield ,  Susan J. Kelly ,  Mark G. P. Saifer ,  Merry R. Sherman

IPC: A61K38/00 ,  C12N9/06 ,  C12N9/02 ,  C12N9/96 ,  A61K47/60

CPC classification number: C12N9/0048 ,  A61K38/00 ,  A61K47/60 ,  C12N9/0046 ,  C12N9/0093 ,  C12N9/96 ,  C12Y107/03003

Abstract: A naturally occurring or recombinant urate oxidase (uricase) covalently coupled to poly(ethylene glycol) or poly(ethylene oxide) (both referred to as PEG), wherein an average of 2 to 10 strands of PEG are conjugated to each uricase subunit and the PEG has an average molecular weight between about 5 kDa and 100 kDa. The resulting PEG-uricase conjugates are substantially non-immunogenic and retain at least 75% of the uricolytic activity of the unmodified enzyme.