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1.发明授权公开(公告)号：US07928287B2
公开(公告)日：2011-04-19
申请号：US10367417
申请日：2003-02-13
申请人： Dwight T. Tomes , Deping Xu , Haiyin Wang , Xiaomu Niu , Igor C. Oliveira
发明人： Dwight T. Tomes , Deping Xu , Haiyin Wang , Xiaomu Niu , Igor C. Oliveira
CPC分类号： C12N15/1082
摘要： The present invention provides for rapid and large scale evaluation of expression of, or function of, nucleotide sequences in plants. The invention comprises three specific components which provide for fast and large scale evaluation of nucleotide sequences. The first component includes delivery in either a single event a library of different engineered vectors or a single engineered vector for a single target nucleotide sequence comprising sequences the function of which is desired to be known in plant cells. Surprisingly, applicants have discovered that, the introduction of multiple vectors to plant cells predominantly results in individual transgenic plants which contain only a single transformation event. The second feature of the invention involves a highly transformable, fast cycling and/or miniature size plant and the final step involves mass scale analysis of T0 plants for various phenotypes and plasmid rescue to identify the nucleotide sequence present in a particular phenotype.
摘要翻译： 本发明提供对植物中核苷酸序列的表达或功能的快速和大规模评估。 本发明包括提供快速和大规模评估核苷酸序列的三个特定组分。 第一组分包括在单一事件中递送不同工程化载体的文库或单个靶核苷酸序列的单一工程载体，其包含其功能期望在植物细胞中已知的序列。 令人惊讶的是，申请人已经发现，将多个载体引入植物细胞主要导致单个转基因植物，其仅包含单个转化事件。 本发明的第二个特征涉及高度可变形，快速循环和/或微型尺寸的植物，并且最终步骤涉及用于各种表型和质粒拯救的T0植物的大规模分析，以鉴定特定表型中存在的核苷酸序列。
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公开(公告)号：US07544857B2
公开(公告)日：2009-06-09
申请号：US11589656
申请日：2006-10-30
申请人： Dilbag Multani , Xiaomu Niu , Dwight T. Tomes , Haiyin Wang , Deping Xu
发明人： Dilbag Multani , Xiaomu Niu , Dwight T. Tomes , Haiyin Wang , Deping Xu
CPC分类号： C07K14/415 , C12N15/8222 , C12N15/8223
摘要： The invention relates to the regulation of gene expression, particularly to methods and compositions for regulating gene expression in plants. Expression cassettes comprising a polynucleotide molecule of interest operably linked to the Brachytic2 (Br2) promoter, or a functional fragment or variant thereof, are provided. Methods of using such expression cassettes, as well as plant cells, plant tissues, plants, and seeds transformed with these expression cassettes, are additionally provided. The methods and compositions find use in regulating the expression of a polynucleotide molecule of interest in a plant.
摘要翻译： 本发明涉及基因表达的调节，特别涉及调节植物中基因表达的方法和组合物。 提供了包含可操作地连接到Brachytic2（Br2）启动子的多核苷酸分子或其功能片段或变体的表达盒。 另外提供了使用这些表达盒以及用这些表达盒转化的植物细胞，植物组织，植物和种子的方法。 所述方法和组合物用于调节植物中感兴趣的多核苷酸分子的表达。
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公开(公告)号：US07148401B2
公开(公告)日：2006-12-12
申请号：US10931077
申请日：2004-08-31
申请人： Dilbag Multani , Xiaomu Niu , Dwight T. Tomes , Haiyin Wang , Deping Xu
发明人： Dilbag Multani , Xiaomu Niu , Dwight T. Tomes , Haiyin Wang , Deping Xu
CPC分类号： C07K14/415 , C12N15/8222 , C12N15/8223
摘要： The invention relates to the regulation of gene expression, particularly to methods and compositions for regulating gene expression in plants. Expression cassettes comprising a polynucleotide molecule of interest operably linked to the Brachytic2 (Br2) promoter, or a functional fragment or variant thereof, are provided. Methods of using such expression cassettes, as well as plant cells, plant tissues, plants, and seeds transformed with these expression cassettes, are additionally provided. The methods and compositions find use in regulating the expression of a polynucleotide molecule of interest in a plant.
摘要翻译： 本发明涉及基因表达的调节，特别涉及调节植物中基因表达的方法和组合物。 提供了包含可操作地连接到Brachytic2（Br2）启动子的多核苷酸分子或其功能片段或变体的表达盒。 另外提供了使用这些表达盒以及用这些表达盒转化的植物细胞，植物组织，植物和种子的方法。 所述方法和组合物用于调节植物中感兴趣的多核苷酸分子的表达。
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公开(公告)号：US06921815B2
公开(公告)日：2005-07-26
申请号：US10109488
申请日：2002-03-28
申请人： Xiaomu Niu , Haiyin Wang , Dwight T. Tomes , Sabine Hantke
发明人： Xiaomu Niu , Haiyin Wang , Dwight T. Tomes , Sabine Hantke
IPC分类号： C07K14/415 , C12N9/06 , C12N15/82 , C12N15/00 , C12N15/09
CPC分类号： C12N9/0032 , C07K14/415 , C12N15/8223 , C12N15/8295
摘要： The present invention provides a composition and method for regulating expression of heterologous nucleotide sequences in a plant. The composition is a novel nucleic acid sequence for a promoter. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequence is also provided. The method comprises transforming a plant cell to contain a heterologous nucleotide sequence operably linked to the promoter of the present invention and regenerating a stably transformed plant from the transformed plant cell.
摘要翻译： 本发明提供了调节植物中异源核苷酸序列表达的组合物和方法。 该组合物是启动子的新型核酸序列。 还提供了使用启动子序列在植物中表达异源核苷酸序列的方法。 该方法包括将植物细胞转化成含有与本发明的启动子可操作地连接的异源核苷酸序列，并从转化的植物细胞再生稳定转化的植物。
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公开(公告)号：US07741534B2
公开(公告)日：2010-06-22
申请号：US12051893
申请日：2008-03-20
申请人： Xiaomu Niu , Haiyin Wang , Dwight T Tomes , Sabine Hantke , Norbert Brugiere
发明人： Xiaomu Niu , Haiyin Wang , Dwight T Tomes , Sabine Hantke , Norbert Brugiere
IPC分类号： C12N15/82
CPC分类号： C07K14/415 , C12N9/0032 , C12N15/8223 , C12N15/8295
摘要： The present invention provides compositions and methods for regulating expression of nucleotide sequences in a plant. Compositions may comprise a novel nucleic acid sequence for a promoter with tissue specificity and/or cytokinin inducibility. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequence is also provided. The method comprises transforming a plant cell to contain a heterologous nucleotide sequence operably linked to the promoter of the present invention and regenerating a stably transformed plant from the transformed plant cell. Other methods provide for downregulation of cytokinin oxidase in a plant.
摘要翻译： 本发明提供调节植物中核苷酸序列表达的组合物和方法。 组合物可以包含用于具有组织特异性和/或细胞分裂素诱导能力的启动子的新型核酸序列。 还提供了使用启动子序列在植物中表达异源核苷酸序列的方法。 该方法包括将植物细胞转化成含有与本发明的启动子可操作地连接的异源核苷酸序列，并从转化的植物细胞再生稳定转化的植物。 其他方法提供植物中细胞分裂素氧化酶的下调。
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公开(公告)号：US08044263B2
公开(公告)日：2011-10-25
申请号：US12819888
申请日：2010-06-21
申请人： Xiaomu Niu , Haiyin Wang , Dwight T. Tomes , Sabine Hantke , Norbert Brugière
发明人： Xiaomu Niu , Haiyin Wang , Dwight T. Tomes , Sabine Hantke , Norbert Brugière
CPC分类号： C07K14/415 , C12N9/0032 , C12N15/8223 , C12N15/8295
摘要： The present invention provides compositions and methods for regulating expression of nucleotide sequences in a plant. Compositions and methods include expression cassettes and transformed plants and provide for downregulation of cytokinin oxidase in a plant.
摘要翻译： 本发明提供调节植物中核苷酸序列表达的组合物和方法。 组合物和方法包括表达盒和转化植物，并提供植物中细胞分裂素氧化酶的下调。
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公开(公告)号：US20100325752A1
公开(公告)日：2010-12-23
申请号：US12819888
申请日：2010-06-21
申请人： Xiaomu Niu , Haiyin Wang , Dwight T. Tomes , Sabine Hantke , Norbert Brugiere
发明人： Xiaomu Niu , Haiyin Wang , Dwight T. Tomes , Sabine Hantke , Norbert Brugiere
CPC分类号： C07K14/415 , C12N9/0032 , C12N15/8223 , C12N15/8295
摘要： The present invention provides compositions and methods for regulating expression of nucleotide sequences in a plant. Compositions and methods include expression cassettes and transformed plants and provide for downregulation of cytokinin oxidase in a plant.
摘要翻译： 本发明提供调节植物中核苷酸序列表达的组合物和方法。 组合物和方法包括表达盒和转化植物，并提供植物中细胞分裂素氧化酶的下调。
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公开(公告)号：US20080172760A1
公开(公告)日：2008-07-17
申请号：US12051893
申请日：2008-03-20
申请人： Xiaomu Niu , Haiyin Wang , Dwight T. Tomes , Sabine Hantke , Norbert Brugiere
发明人： Xiaomu Niu , Haiyin Wang , Dwight T. Tomes , Sabine Hantke , Norbert Brugiere
CPC分类号： C07K14/415 , C12N9/0032 , C12N15/8223 , C12N15/8295
摘要： The present invention provides compositions and methods for regulating expression of nucleotide sequences in a plant. Compositions may comprise a novel nucleic acid sequence for a promoter with tissue specificity and/or cytokinin inducibility. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequence is also provided. The method comprises transforming a plant cell to contain a heterologous nucleotide sequence operably linked to the promoter of the present invention and regenerating a stably transformed plant from the transformed plant cell. Other methods provide for downregulation of cytokinin oxidase in a plant.
摘要翻译： 本发明提供调节植物中核苷酸序列表达的组合物和方法。 组合物可以包含用于具有组织特异性和/或细胞分裂素诱导能力的启动子的新型核酸序列。 还提供了使用启动子序列在植物中表达异源核苷酸序列的方法。 该方法包括将植物细胞转化成含有与本发明的启动子可操作地连接的异源核苷酸序列，并从转化的植物细胞再生稳定转化的植物。 其他方法提供植物中细胞分裂素氧化酶的下调。
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公开(公告)号：US07371925B2
公开(公告)日：2008-05-13
申请号：US11074144
申请日：2005-03-07
申请人： Xiaomu Niu , Haiyin Wang , Dwight T. Tomes , Sabine Hantke , Norbert Brugière
发明人： Xiaomu Niu , Haiyin Wang , Dwight T. Tomes , Sabine Hantke , Norbert Brugière
CPC分类号： C07K14/415 , C12N9/0032 , C12N15/8223 , C12N15/8295
摘要： The present invention provides compositions and methods for regulating expression of nucleotide sequences in a plant. Compositions may comprise a novel nucleic acid sequence for a promoter with tissue specificity and/or cytokinin inducibility. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequence is also provided. The method comprises transforming a plant cell to contain a heterologous nucleotide sequence operably linked to the promoter of the present invention and regenerating a stably transformed plant from the transformed plant cell. Other methods provide for downregulation of cytokinin oxidase in a plant.
摘要翻译： 本发明提供调节植物中核苷酸序列表达的组合物和方法。 组合物可以包含用于具有组织特异性和/或细胞分裂素诱导能力的启动子的新型核酸序列。 还提供了使用启动子序列在植物中表达异源核苷酸序列的方法。 该方法包括将植物细胞转化成含有与本发明的启动子可操作地连接的异源核苷酸序列，并从转化的植物细胞再生稳定转化的植物。 其他方法提供植物中细胞分裂素氧化酶的下调。
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公开(公告)号：US20050204432A1
公开(公告)日：2005-09-15
申请号：US10931077
申请日：2004-08-31
申请人： Dilbag Multani , Xiaomu Niu , Dwight Tomes , Haiyin Wang , Deping Xu
发明人： Dilbag Multani , Xiaomu Niu , Dwight Tomes , Haiyin Wang , Deping Xu
CPC分类号： C07K14/415 , C12N15/8222 , C12N15/8223
摘要： The invention relates to the regulation of gene expression, particularly to methods and compositions for regulating gene expression in plants. Expression cassettes comprising a polynucleotide molecule of interest operably linked to the Brachytic2 (Br2) promoter, the Dwarf3 (Dw3) promoter, or a functional fragment or variant thereof, are provided. Methods of using such expression cassettes, as well as plant cells, plant tissues, plants, and seeds transformed with these expression cassettes, are additionally provided. The methods and compositions find use in regulating the expression of a polynucleotide molecule of interest in a plant.
摘要翻译： 本发明涉及基因表达的调节，特别涉及用于调节植物中基因表达的方法和组合物。 提供了包含与Brachytic2（Br2）启动子，Dw3（Dw3）启动子或其功能片段或变体可操作连接的目的多核苷酸分子的表达盒。 另外提供了使用这些表达盒以及用这些表达盒转化的植物细胞，植物组织，植物和种子的方法。 所述方法和组合物用于调节植物中感兴趣的多核苷酸分子的表达。
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