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公开(公告)号：US10676754B2
公开(公告)日：2020-06-09
申请号：US15325116
申请日：2015-07-01
发明人： Vesna Djukanovic , Spencer Charles Jones , Zhan-Bin Liu , Michael Lassner , Leszek Aleksander Lyznik
摘要： The present disclosure includes the production of a mutant plant resistant to an herbicide of the phosphonomethylglycine family, e.g. glyphosate. Compositions and methods are provided for editing a nucleotide sequence of interest in a cell employing a guide polynucleotide/Cas endonuclease system, wherein the Cas endonuclease is guided by a guide polynucleotide to recognize and optionally introduce a double strand break at a specific target site into the genome of a cell. The nucleotide sequence of interest to be edited can be located within or outside the target site that is recognized by a Cas endonuclease. More specifically, compositions and methods are provided for editing an enolpyruvylshikimate-3-phosphate synthase (EPSPS) nucleotide sequence in a cell. The methods and compositions employ a guide polynucleotide/Cas endonuclease system to provide for an effective system for editing EPSPS nucleotide sequences of within the genome of a cell. Also provided are compositions and methods for the production of glyphosate tolerant plant cells, plants explants, seeds and grain.
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2.发明申请PLANT GENOME MODIFICATION USING GUIDE RNA/CAS ENDONUCLEASE SYSTEMS AND METHODS OF USE 审中-公开使用指南RNA / CAS内切酶系统进行植物基因组修饰及其使用方法公开(公告)号：US20150082478A1
公开(公告)日：2015-03-19
申请号：US14463687
申请日：2014-08-20
发明人： Andrew Mark Cigan , Saverio Carl Falco , Huirong Gao , Zhongsen Li , Zhan-Bin Liu , L. Aleksander Lyznik , Jinrui Shi , Sergei Svitashev , Joshua K. Young
CPC分类号： C12N15/8216 , C12N15/113 , C12N15/81 , C12N15/8213 , C12N15/8241 , C12N15/8245 , C12N15/8247 , C12N15/8261 , C12N15/8273 , C12N15/8274 , C12N15/8279 , C12N15/8286 , C12N2310/10 , C12N2310/20 , C12N2310/315 , C12N2310/3231 , C12N2310/3341 , C12Q1/6895 , C12Q2600/13 , C12Q2600/156 , C12N2310/321 , C12N2310/3521 , C12N2310/322 , C12N2310/3533
摘要： Compositions and methods are provided for genome modification of a target sequence in the genome of a plant or plant cell. The methods and compositions employ a guide RNA/Cas endonuclease system to provide an effective system for modifying or altering target sites within the genome of a plant, plant cell or seed. Also provided are compositions and methods employing a guide polynucleotide/Cas endonuclease system for genome modification of a nucleotide sequence in the genome of a cell or organism, for gene editing, and/or for inserting or deleting a polynucleotide of interest into or from the genome of a cell or organism. Once a genomic target site is identified, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest. Breeding methods and methods for selecting plants utilizing a two component RNA guide and Cas endonuclease system are also disclosed. Compositions and methods are also provided for editing a nucleotide sequence in the genome of a cell.
摘要翻译： 提供组合物和方法用于基因组修饰植物或植物细胞基因组中的靶序列。 方法和组合物采用指导性RNA / Cas内切核酸酶系统来提供用于修饰或改变植物，植物细胞或种子基因组内的靶位点的有效系统。 还提供了使用引导多核苷酸/ Cas内切核酸酶系统用于基因组修饰细胞或生物体基因组中的核苷酸序列，用于基因编辑和/或用于在基因组中插入或缺失目标多核苷酸或从基因组中插入或缺失目的多核苷酸的组合物和方法 的细胞或生物体。 一旦识别出基因组靶位点，就可以使用多种方法来进一步修饰靶位点，使得它们含有多种感兴趣的多核苷酸。 还公开了利用双组分RNA引物和Cas内切核酸酶系统选择植物的育种方法和方法。 还提供组合物和方法用于编辑细胞基因组中的核苷酸序列。
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公开(公告)号：US20180305706A1
公开(公告)日：2018-10-25
申请号：US16007529
申请日：2018-06-13
发明人： Andrew Mark Cigan , Saverio Carl Falco , Huirong Gao , Michael Lassner , Derek Jantz , Zhongsen Li , Zhan-Bin Liu , Sergei Svitashev , James Jefferson Smith
CPC分类号： C12N15/8202 , A01H1/06 , C12N9/22 , C12N15/8213
摘要： Methods for producing in a plant a complex transgenic trait locus comprising at least two altered target sequences in a genomic region of interest are disclosed. The methods involve the use of two or more double-strand-break-inducing agents, each of which can cause a double-strand break in a target sequence in the genomic region of interest which results in an alteration in the target sequence. Also disclosed are complex transgenic trait loci in plants. A complex transgenic trait locus comprises at least two altered target sequences that are genetically linked to a polynucleotide of interest. Plants, plant cells, plant parts, and seeds comprising one or more complex transgenic trait loci are also disclosed.
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4.发明授权Compositions and methods comprising sequences having meganuclease activity 有权包含具有大范围核酸酶活性的序列的组合物和方法公开(公告)号：US09499827B2
公开(公告)日：2016-11-22
申请号：US13886317
申请日：2013-05-03
发明人： Ericka Bermudez , Andrew Mark Cigan , James English , Saverio Carl Falco , Huirong Gao , Lu Liu , Zhan-Bin Liu , Azalea Ong , Sergei Svitashev , Joshua K Young
CPC分类号： C12N9/22 , C12N9/16 , C12N15/81 , C12N15/8213 , C12Y301/00
摘要： Compositions and methods comprising polynucleotides and polypeptides having meganuclease activity are provided. Further provided are nucleic acid constructs, yeast, plants, plant cells, explants, seeds and grain having the meganuclease sequences. Various methods of employing the meganuclease sequences are provided. Such methods include, for example, methods for producing a meganuclease with increased activity at a wide range of temperatures, methods for producing a yeast, plant, plant cell, explant or seed comprising a meganuclease with increased activity.
摘要翻译： 提供了包含具有大范围核酸酶活性的多核苷酸和多肽的组合物和方法。 还提供了具有大范围核酸酶序列的核酸构建体，酵母，植物，植物细胞，外植体，种子和颗粒。 提供了使用大范围核酸酶序列的各种方法。 这样的方法包括例如用于生产在宽温度范围内具有增加的活性的大范围核酸酶的方法，用于生产酵母，植物，植物细胞，外植体或包含具有增加的活性的大范围核酸酶的种子的方法。
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公开(公告)号：US20180371479A1
公开(公告)日：2018-12-27
申请号：US16054156
申请日：2018-08-03
发明人： Andrew Mark Cigan , Saverio Carl Falco , Huirong Gao , Zhongsen Li , Zhan-Bin Liu , L. Aleksander Lyznik , Jinrui Shi , Sergei Svitashev , Joshua K. Young
IPC分类号： C12N15/82 , C12N15/113 , C12N15/81 , C12Q1/6895
摘要： Compositions and methods are provided for genome modification of a target sequence in the genome of a plant or plant cell. The methods and compositions employ a guide RNA/Cas endonuclease system to provide an effective system for modifying or altering target sites within the genome of a plant, plant cell or seed. Also provided are compositions and methods employing a guide polynucleotide/Cas endonuclease system for genome modification of a nucleotide sequence in the genome of a cell or organism, for gene editing, and/or for inserting or deleting a polynucleotide of interest into or from the genome of a cell or organism. Once a genomic target site is identified, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest. Breeding methods and methods for selecting plants utilizing a two component RNA guide and Cas endonuclease system are also disclosed. Compositions and methods are also provided for editing a nucleotide sequence in the genome of a cell.
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公开(公告)号：US20170306349A1
公开(公告)日：2017-10-26
申请号：US15325116
申请日：2015-07-01
发明人： Vesna Djukanovic , Spencer Charles Jones , Zhan-Bin Liu , Michael Lassner , Leszek Aleksander Lyznik
CPC分类号： C12N15/8275 , A01H1/02 , A01H4/008 , C12N15/8213 , C12N15/8241 , C12N2310/20 , C12Y301/00
摘要： The present disclosure includes the production of a mutant plant resistant to an herbicide of the phosphonomethylglycine family, e.g. glyphosate. Compositions and methods are provided for editing a nucleotide sequence of interest in a cell employing a guide polynucleotide/Cas endonuclease system, wherein the Cas endonuclease is guided by a guide polynucleotide to recognize and optionally introduce a double strand break at a specific target site into the genome of a cell. The nucleotide sequence of interest to be edited can be located within or outside the target site that is recognized by a Cas endonuclease. More specifically, compositions and methods are provided for editing an enolpyruvylshikimate-3-phosphate synthase (EPSPS) nucleotide sequence in a cell. The methods and compositions employ a guide polynucleotide/Cas endonuclease system to provide for an effective system for editing EPSPS nucleotide sequences of within the genome of a cell. Also provided are compositions and methods for the production of glyphosate tolerant plant cells, plants explants, seeds and grain.
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公开(公告)号：US11560568B2
公开(公告)日：2023-01-24
申请号：US15510037
申请日：2015-08-31
发明人： Andrew Mark Cigan , Huirong Gao , Zhan-Bin Liu , Jasdeep Mutti , Dean Podlich , Christopher Jay Scelonge
IPC分类号： C12N15/82
摘要： Compositions and methods are provided for introducing transgenic target sites for Site Specific Integration (SSI) and/or polynucleotides of interest into at least one double-strand break target site of a double-strand-break inducing agent in a genomic window of a plant genome. Also provided are methods and compositions for producing a complex trait locus in a genomic window of a plant comprising at least one transgenic target site for site specific integration integrated in at least double-strand-break target site. The double-strand-break target site can be, but is not limited to, a target site for a zinc finger endonuclease, an engineered endonuclease, a meganuclease, a TALENs and/or a Cas endonuclease. The genomic window of said plant can comprise at least one genomic locus of interest such as a trait cassette, a transgene, a mutated gene, a native gene, an edited gene or a site-specific integration (SSI) target site.
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公开(公告)号：US11459576B2
公开(公告)日：2022-10-04
申请号：US16925973
申请日：2020-07-10
发明人： Andrew Mark Cigan , Saverio Carl Falco , Huirong Gao , Michael Lassner , Derek Jantz , Zhongsen Li , Zhan-Bin Liu , Sergei Svitashev , James Jefferson Smith
摘要： Methods for producing in a plant a complex transgenic trait locus comprising at least two altered target sequences in a genomic region of interest are disclosed. The methods involve the use of two or more double-strand-break-inducing agents, each of which can cause a double-strand break in a target sequence in the genomic region of interest which results in an alteration in the target sequence. Also disclosed are complex transgenic trait loci in plants. A complex transgenic trait locus comprises at least two altered target sequences that are genetically linked to a polynucleotide of interest. Plants, plant cells, plant parts, and seeds comprising one or more complex transgenic trait loci are also disclosed.
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公开(公告)号：US10822610B2
公开(公告)日：2020-11-03
申请号：US16007529
申请日：2018-06-13
发明人： Andrew Mark Cigan , Saverio Carl Falco , Huirong Gao , Michael Lassner , Derek Jantz , Zhongsen Li , Zhan-Bin Liu , Sergei Svitashev , James Jefferson Smith
摘要： Methods for producing in a plant a complex transgenic trait locus comprising at least two altered target sequences in a genomic region of interest are disclosed. The methods involve the use of two or more double-strand-break-inducing agents, each of which can cause a double-strand break in a target sequence in the genomic region of interest which results in an alteration in the target sequence. Also disclosed are complex transgenic trait loci in plants. A complex transgenic trait locus comprises at least two altered target sequences that are genetically linked to a polynucleotide of interest. Plants, plant cells, plant parts, and seeds comprising one or more complex transgenic trait loci are also disclosed.
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公开(公告)号：US09909110B2
公开(公告)日：2018-03-06
申请号：US15289993
申请日：2016-10-11
发明人： Ericka Bermudez , Andrew Mark Cigan , James English , Saverio Carl Falco , Huirong Gao , Lu Liu , Zhan-Bin Liu , Azalea Ong , Sergei Svitashev , Joshua K Young
CPC分类号： C12N9/22 , C12N9/16 , C12N15/81 , C12N15/8213 , C12Y301/00
摘要： Compositions and methods comprising polynucleotides and polypeptides having meganuclease activity are provided. Further provided are nucleic acid constructs, yeast, plants, plant cells, explants, seeds and grain having the meganuclease sequences. Various methods of employing the meganuclease sequences are provided. Such methods include, for example, methods for producing a meganuclease with increased activity at a wide range of temperatures, methods for producing a yeast, plant, plant cell, explant or seed comprising a meganuclease with increased activity.
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