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公开(公告)号：US11312969B2
公开(公告)日：2022-04-26
申请号：US16551195
申请日：2019-08-26
摘要： Compositions and methods are provided for stacking multiple independent transgenic loci into the genome of a plant. Compositions include plants, seeds or plant cells comprising at least one transgenic target site and at least one genomic locus of interest integrated at different genomic sites within a genomic window. Plant breeding techniques can be employed such that the transgenic target site and the genomic locus of interest can be bred together. In this way, multiple independent transgene integrations can be generated within a genomic window to create a complex trait locus. The complex trait locus is designed such that the transgenic target sites and/or genomic loci of interest can segregate independently of each other, thus providing the benefit of altering a complex trait locus by breeding-in and breeding-away specific elements. Various methods can also be employed to modify the target sites such that they contain a variety of polynucleotides of interest.
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公开(公告)号：US10676754B2
公开(公告)日：2020-06-09
申请号：US15325116
申请日：2015-07-01
发明人： Vesna Djukanovic , Spencer Charles Jones , Zhan-Bin Liu , Michael Lassner , Leszek Aleksander Lyznik
摘要： The present disclosure includes the production of a mutant plant resistant to an herbicide of the phosphonomethylglycine family, e.g. glyphosate. Compositions and methods are provided for editing a nucleotide sequence of interest in a cell employing a guide polynucleotide/Cas endonuclease system, wherein the Cas endonuclease is guided by a guide polynucleotide to recognize and optionally introduce a double strand break at a specific target site into the genome of a cell. The nucleotide sequence of interest to be edited can be located within or outside the target site that is recognized by a Cas endonuclease. More specifically, compositions and methods are provided for editing an enolpyruvylshikimate-3-phosphate synthase (EPSPS) nucleotide sequence in a cell. The methods and compositions employ a guide polynucleotide/Cas endonuclease system to provide for an effective system for editing EPSPS nucleotide sequences of within the genome of a cell. Also provided are compositions and methods for the production of glyphosate tolerant plant cells, plants explants, seeds and grain.
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公开(公告)号：US09206432B2
公开(公告)日：2015-12-08
申请号：US14640163
申请日：2015-03-06
IPC分类号： C12N15/00 , C07H21/04 , C12N15/82 , C12N15/63 , C07K14/195 , C07K14/245
CPC分类号： C07K14/245 , C07K14/195 , C12N15/635 , C12N15/8217 , C12N15/8238 , C12N15/8278
摘要： Compositions and methods relating to the use of sulfonylurea-responsive repressors are provided. Compositions include polypeptides that specifically bind to an operator, wherein the specific binding is regulated by a sulfonylurea compound. Compositions also include polynucleotides encoding the polypeptides as well as constructs, vectors, prokaryotic and eukaryotic cells, and eukaryotic organisms including plants and seeds comprising the polynucleotide, and/or produced by the methods. Also provided are methods to provide a sulfonylurea-responsive repressor to a cell or organism, and to regulate expression of a polynucleotide of interest in a cell or organism, including a plant or plant cell.
摘要翻译： 提供了与使用磺酰脲类阻遏物相关的组合物和方法。 组合物包括特异性结合操作者的多肽，其中特异性结合受磺酰脲化合物调节。 组合物还包括编码多肽的多核苷酸以及构建体，载体，原核和真核细胞，以及真核生物，包括包含多核苷酸的植物和种子，和/或由该方法产生的。 还提供了向细胞或生物体提供磺酰脲反应性阻遏物并调节目标多核苷酸在包括植物或植物细胞的细胞或生物体中的表达的方法。
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公开(公告)号：US09617559B2
公开(公告)日：2017-04-11
申请号：US14501240
申请日：2014-09-30
发明人： Rafael Herrmann , Michael Lassner , Albert L. Lu , Mark Edward Nelson , James Kevin Presnail , Janet A. Rice
IPC分类号： C12N15/82 , C12N15/113
CPC分类号： C12N15/8286 , A01N65/20 , A01N65/44 , C12N15/113 , C12N2310/11 , C12N2310/14 , C12N2310/531 , C12N2320/32 , Y02A40/162
摘要： Methods and compositions are provided which employ a silencing element that, when ingested by a pest, such as a pest from the Lepidoptera order, they are capable of decreasing the expression of a target sequence in the pest. Further provided are silencing elements which when ingested by the pest decrease the level of the target polypeptide and thereby control the pest. In specific embodiment, the pest is Spodoptera frugiperda. Plants, plant part, bacteria and other host cells comprising the silencing elements or an active variant or fragment thereof of the invention are also provided.
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5.发明申请COMPOSITIONS AND METHODS FOR THE SUPPRESSION OF TARGET POLYNUCLEOTIDES FROM LYGUS 审中-公开用于抑制来自LYGUS的目标多核苷酸的组合物和方法公开(公告)号：US20140343131A1
公开(公告)日：2014-11-20
申请号：US14455368
申请日：2014-08-08
发明人： RAFAEL HERRMANN , Michael Lassner , Albert Laurence Lu , Mark Edward Nelson , James Kevin Presnail , Janet Ann Rice
IPC分类号： C12N15/82 , C12N15/113
CPC分类号： C12N15/8286 , A01N57/16 , C12N15/113 , C12N15/8218 , C12N2310/141 , C12N2310/531 , Y02A40/162
摘要： Methods and compositions are provided which employ a silencing element that, when ingested by a pest, such as a pest from the Lygus genus, they are capable of decreasing the expression of a target sequence in the pest. In specific embodiments, the decrease in expression of the target sequence controls the pest and thereby the methods and compositions are capable of limiting damage to a plant. The present invention provides various target polynucleotides from specific polypeptide families as disclosed herein, and further provides target polynucleotides set forth in SEQ ID NOS:1-21 or active variants thereof, wherein a decrease in expression of one or more the sequences in the target pest controls the pest (i.e., has insecticidal activity). Further provided are silencing elements which when ingested by the pest decrease the level of the target polypeptide and thereby control the pest. In specific embodiment, the pest is Lygus Hesperus. Plants, plant part, bacteria and other host cells comprising the silencing elements or an active variant or fragment thereof of the invention are also provided.
摘要翻译： 提供了使用沉积元件的方法和组合物，当沉积元件被害虫摄取时，例如来自莱古氏属属的害虫，它们能够降低害虫中靶序列的表达。 在具体实施方案中，靶序列的表达降低控制有害生物，因此方法和组合物能够限制对植物的损害。 本发明提供了本文公开的来自特定多肽家族的多种目标多核苷酸，并进一步提供了SEQ ID NO：1-21所示的靶多核苷酸或其活性变体，其中目标害虫中一种或多种序列的表达降低 控制害虫（即具有杀虫活性）。 进一步提供的是沉积元件，当受害虫摄入时，降低靶多肽的水平，从而控制害虫。 在具体实施方案中，有害生物是黄瓜（Lygus Hesperus）。 还提供了包含本发明的沉默元件或其活性变体或片段的植物，植物部分，细菌和其它宿主细胞。
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公开(公告)号：US11459576B2
公开(公告)日：2022-10-04
申请号：US16925973
申请日：2020-07-10
发明人： Andrew Mark Cigan , Saverio Carl Falco , Huirong Gao , Michael Lassner , Derek Jantz , Zhongsen Li , Zhan-Bin Liu , Sergei Svitashev , James Jefferson Smith
摘要： Methods for producing in a plant a complex transgenic trait locus comprising at least two altered target sequences in a genomic region of interest are disclosed. The methods involve the use of two or more double-strand-break-inducing agents, each of which can cause a double-strand break in a target sequence in the genomic region of interest which results in an alteration in the target sequence. Also disclosed are complex transgenic trait loci in plants. A complex transgenic trait locus comprises at least two altered target sequences that are genetically linked to a polynucleotide of interest. Plants, plant cells, plant parts, and seeds comprising one or more complex transgenic trait loci are also disclosed.
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公开(公告)号：US11236347B2
公开(公告)日：2022-02-01
申请号：US15756023
申请日：2016-08-26
发明人： Ajith Anand , Steven Henry Bass , Sean M. Bertain , Hyeon-Je Cho , Anthony J. Kinney , Theodore M. Klein , Michael Lassner , Kevin E. McBride , York Moy , Barbara Ann Marie Rosen , Jun-Zhi Wei
IPC分类号： C12N15/82 , C07K14/195 , C12N15/65
摘要： Methods and compositions for Ochrobactrum-mediated transformation of plants are provided. Methods include but are not limited to using an Ochrobactrum strain to transfer a polynucleotide of interest to a plant cell. These include VirD2-dependent methods. Compositions include an Ochrobactrum strain, transfer DNAs, constructs and/or plasmids. These include Ochrobactrum strains having a plasmid comprising one or more virulence gene(s), border region, and/or origin of replication. Plant cells, tissues, plants, and seeds comprising a polynucleotide of interest produced by the methods are also provided.
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公开(公告)号：US10822610B2
公开(公告)日：2020-11-03
申请号：US16007529
申请日：2018-06-13
发明人： Andrew Mark Cigan , Saverio Carl Falco , Huirong Gao , Michael Lassner , Derek Jantz , Zhongsen Li , Zhan-Bin Liu , Sergei Svitashev , James Jefferson Smith
摘要： Methods for producing in a plant a complex transgenic trait locus comprising at least two altered target sequences in a genomic region of interest are disclosed. The methods involve the use of two or more double-strand-break-inducing agents, each of which can cause a double-strand break in a target sequence in the genomic region of interest which results in an alteration in the target sequence. Also disclosed are complex transgenic trait loci in plants. A complex transgenic trait locus comprises at least two altered target sequences that are genetically linked to a polynucleotide of interest. Plants, plant cells, plant parts, and seeds comprising one or more complex transgenic trait loci are also disclosed.
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9.发明申请METHODS FOR PRODUCING GENETIC MODIFICATIONS IN A PLANT GENOME WITHOUT INCORPORATING A SELECTABLE TRANSGENE MARKER, AND COMPOSITIONS THEREOF 审中-公开在不配合选择性转基因标记的情况下在植物基因组中生产遗传修饰的方法及其组合物公开(公告)号：US20160208271A1
公开(公告)日：2016-07-21
申请号：US14913607
申请日：2014-08-20
IPC分类号： C12N15/82
CPC分类号： C12N15/8216 , C12N15/113 , C12N15/81 , C12N15/8213 , C12N15/8241 , C12N15/8245 , C12N15/8247 , C12N15/8261 , C12N15/8273 , C12N15/8274 , C12N15/8279 , C12N15/8286 , C12N2310/10 , C12N2310/20 , C12N2310/315 , C12N2310/3231 , C12N2310/3341 , C12Q1/6895 , C12Q2600/13 , C12Q2600/156 , C12N2310/321 , C12N2310/3521 , C12N2310/322 , C12N2310/3533
摘要： Compositions and methods are provided for genome modification of a target sequence in the genome of a plant or plant cell, without incorporating a selectable transgene marker. The methods and compositions employ a guide polynucleotide/Cas endonuclease system to provide an effective system for modifying or altering target sites within the genome of a plant, plant cell or seed, without incorporating a selectable transgene marker. Once a genomic target site is identified, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest. Breeding methods and methods for selecting plants utilizing a guide polynucleotide/Cas endonuclease system are also disclosed. Compositions and methods are also provided for editing a nucleotide sequence in the genome of a cell, without incorporating a selectable transgene marker.
摘要翻译： 提供组合物和方法用于在植物或植物细胞的基因组中靶基因组的基因组修饰，而不加入可选择的转基因标记。 方法和组合物采用引导多核苷酸/ Cas内切核酸酶系统来提供用于修饰或改变植物，植物细胞或种子基因组内的靶位点的有效系统，而不需要并入可选择的转基因标记。 一旦识别出基因组靶位点，就可以使用多种方法来进一步修饰靶位点，使得它们含有多种感兴趣的多核苷酸。 还公开了利用引导多核苷酸/ Cas内切核酸酶系统选择植物的育种方法和方法。 还提供组合物和方法用于编辑细胞基因组中的核苷酸序列，而不包含可选择的转基因标记。
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公开(公告)号：US20170306349A1
公开(公告)日：2017-10-26
申请号：US15325116
申请日：2015-07-01
发明人： Vesna Djukanovic , Spencer Charles Jones , Zhan-Bin Liu , Michael Lassner , Leszek Aleksander Lyznik
CPC分类号： C12N15/8275 , A01H1/02 , A01H4/008 , C12N15/8213 , C12N15/8241 , C12N2310/20 , C12Y301/00
摘要： The present disclosure includes the production of a mutant plant resistant to an herbicide of the phosphonomethylglycine family, e.g. glyphosate. Compositions and methods are provided for editing a nucleotide sequence of interest in a cell employing a guide polynucleotide/Cas endonuclease system, wherein the Cas endonuclease is guided by a guide polynucleotide to recognize and optionally introduce a double strand break at a specific target site into the genome of a cell. The nucleotide sequence of interest to be edited can be located within or outside the target site that is recognized by a Cas endonuclease. More specifically, compositions and methods are provided for editing an enolpyruvylshikimate-3-phosphate synthase (EPSPS) nucleotide sequence in a cell. The methods and compositions employ a guide polynucleotide/Cas endonuclease system to provide for an effective system for editing EPSPS nucleotide sequences of within the genome of a cell. Also provided are compositions and methods for the production of glyphosate tolerant plant cells, plants explants, seeds and grain.
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