公开(公告)号：US09988670B2

公开(公告)日：2018-06-05

申请号：US14964716

申请日：2015-12-10

申请人： ELITechGroup B.V.

发明人： Irina A. Afonina ,  Yevgeniy S. Belousov

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6806 ,  C12Q1/6853 ,  C12Q1/689 ,  C12Q2600/112 ,  C12Q2600/156 ,  Y02A50/451 ,  C12Q2525/161 ,  C12Q2561/113 ,  C12Q2563/107 ,  C12Q2565/1015 ,  C12Q2565/107

摘要： Primers and probes specific to genes encoding carbapenem-resistant Enterobacteriaceae (CREs) that include KPC (Klebsiella pneumoniae carbapenemase), NDM-1 (New Delhi Metallo-beta-lactamase), VIM (Verona Integron-Mediated Metallo-β-lactamase), IMP-type carbapenemase and OXA 48 (oxacillinase), that cause resistance in Enterobacteriaceae bacteria, are described herein, with methods and kits for using these primers and probes to detect target nucleic acids. In the methods described, nucleic acids present in a clinical or test sample obtained from a biological sample or tissue suspected of containing the the NDM1, KPC, IMP, VIM and OXA genes are amplified and corresponding sequences for the NDM1, KPC, IMP, VIM and OXA genes are detected. The amplified nucleic acid can be detected by a variety of state of the art methods, including fluorescence resonance energy transfer (FRET), radiolabels, enzyme labels, and the like.

公开(公告)号：US09932643B2

公开(公告)日：2018-04-03

申请号：US15454794

申请日：2017-03-09

申请人： ElitechGroup B.V.

发明人： Irina A. Afonina ,  Yevgeniy S. Belousov ,  Walt Mahoney

IPC分类号： C12Q1/68 ,  C07H21/04

CPC分类号： C12Q1/689 ,  C12Q1/6853 ,  C12Q1/686 ,  C12Q2600/156 ,  C12Q2600/158 ,  C12Q2525/161 ,  C12Q2537/143 ,  C12Q2545/114 ,  C12Q2561/113

摘要： The present methods pertain to amplifying and/or detecting Staphylococcus aureus (“SA”) and methicillin-resistant Staphylococcus aureus (“MRSA”) nucleic acids based on a combined detection of ldh1 as a SA marker and mecA as a MRSA marker. In certain embodiments the methods also pertain to amplifying and/or detecting one or more SCCmec integration sites or bridge regions. Primers and probes are suitable to be used in the present methods to detect SA and MRSA simultaneously in a single reaction or in separate reactions. The amplified nucleic acid can be detected by a variety of state of the art methods, including fluorescence resonance energy transfer (“FRET”), radiolabels, enzyme labels, and the like.

公开(公告)号：US10266903B2

公开(公告)日：2019-04-23

申请号：US14957754

申请日：2015-12-03

申请人： ELITechGroup B.V.

发明人： Irina A. Afonina ,  Yevgeniy S. Belousov

IPC分类号： C12P19/34 ,  C12Q1/689 ,  C12Q1/6851 ,  C12Q1/6853 ,  G06F3/0488 ,  G06Q20/10

摘要： Primers and probes specific to the genes encoding extended spectrum beta-lactamase that involves CTX-M groups 1 and 9 that cause extended beta-lactamase resistance in bacteria are described herein, with methods and kits for using these primers and probes to detect CTX-M groups 1 and 9 nucleic acids. In the methods described, nucleic acids present in a clinical or test sample obtained from a biological sample or tissue suspected of containing the CTX-M groups 1 and 9 gene are amplified and corresponding sequences for CTX-M groups 1 and 9 are detected. The amplified nucleic acid can be detected by a variety of state of the art methods, including fluorescence resonance energy transfer (FRET), radiolabels, enzyme labels, and the like.

公开(公告)号：US20160168625A1

公开(公告)日：2016-06-16

申请号：US14964716

申请日：2015-12-10

申请人： ELITechGroup B.V.

发明人： Irina A. Afonina ,  Yevgeniy S. Belousov

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6806 ,  C12Q1/6853 ,  C12Q1/689 ,  C12Q2600/112 ,  C12Q2600/156 ,  Y02A50/451 ,  C12Q2525/161 ,  C12Q2561/113 ,  C12Q2563/107 ,  C12Q2565/1015 ,  C12Q2565/107

摘要： Primers and probes specific to genes encoding carbapenem-resistant Enterobacteriaceae (CREs) that include KPC (Klebsiella pneumoniae carbapenemase), NDM-1 (New Delhi Metallo-beta-lactamase), VIM (Verona Integron-Mediated Metallo-β-lactamase), IMP-type carbapenemase and OXA 48 (oxacillinase), that cause resistance in Enterobacteriaceae bacteria, are described herein, with methods and kits for using these primers and probes to detect target nucleic acids. In the methods described, nucleic acids present in a clinical or test sample obtained from a biological sample or tissue suspected of containing the the NDM1, KPC, IMP, VIM and OXA genes are amplified and corresponding sequences for the NDM1, KPC, IMP, VIM and OXA genes are detected. The amplified nucleic acid can be detected by a variety of state of the art methods, including fluorescence resonance energy transfer (FRET), radiolabels, enzyme labels, and the like.

摘要翻译： 包含KPC（肺炎克雷伯杆菌碳青霉烯酶），NDM-1（新德里金属-β-内酰胺酶），VIM（维罗纳整合 - 介导的金属 - 内酰胺酶）的N-氨基苯甲酸耐药性肠杆菌科（CRE）的基因的引物和探针， 本文描述了在肠杆菌科细菌中引起抗性的IMP型碳青霉烯酶和OXA 48（奥沙西林酶），其中使用这些引物和探针来检测靶核酸的方法和试剂盒。 在所述方法中，存在于怀疑含有NDM1，KPC，IMP，VIM和OXA基因的生物样品或组织获得的临床或测试样品中的核酸被扩增，并且NDM1，KPC，IMP，VIM的相应序列 并检测OXA基因。 扩增的核酸可以通过各种现有技术方法检测，包括荧光共振能量转移（FRET），放射性标记，酶标记等。

公开(公告)号：US20160168628A1

公开(公告)日：2016-06-16

申请号：US14957754

申请日：2015-12-03

申请人： ELITechGroup B.V.

发明人： Irina A. Afonina ,  Yevgeniy S. Belousov

IPC分类号： C12Q1/68

CPC分类号： C12Q1/689 ,  C12Q1/6851 ,  C12Q1/6853 ,  C12Q2600/158 ,  C12Q2600/16 ,  G06F3/0488 ,  G06Q20/1085 ,  C12Q2525/161 ,  C12Q2561/113

摘要： Primers and probes specific to the genes encoding extended spectrum beta-lactamase that involves CTX-M groups 1 and 9 that cause extended beta-lactamase resistance in bacteria are described herein, with methods and kits for using these primers and probes to detect CTX-M groups 1 and 9 nucleic acids. In the methods described, nucleic acids present in a clinical or test sample obtained from a biological sample or tissue suspected of containing the CTX-M groups 1 and 9 gene are amplified and corresponding sequences for CTX-M groups 1 and 9 are detected. The amplified nucleic acid can be detected by a variety of state of the art methods, including fluorescence resonance energy transfer (FRET), radiolabels, enzyme labels, and the like.

摘要翻译： 本文描述了涉及编码扩展光谱β-内酰胺酶的基因的引物和探针，其涉及引起细菌中延长的β-内酰胺酶抗性的CTX-M组1和9，其中使用这些引物和探针来检测CTX-M 第1组和第9组核酸。 在所述方法中，扩增了从怀疑含有CTX-M组1和9基因的生物样品或组织获得的临床或试验样品中存在的核酸，并检测CTX-M组1和9的相应序列。 扩增的核酸可以通过各种现有技术方法检测，包括荧光共振能量转移（FRET），放射性标记，酶标记等。

公开(公告)号：US20170183717A1

公开(公告)日：2017-06-29

申请号：US15454794

申请日：2017-03-09

申请人： ElitechGroup B.V.

发明人： Irina A. Afonina ,  Yevgeniy S. Belousov ,  Walt Mahoney

IPC分类号： C12Q1/68

CPC分类号： C12Q1/689 ,  C12Q1/6853 ,  C12Q1/686 ,  C12Q2600/156 ,  C12Q2600/158 ,  C12Q2525/161 ,  C12Q2537/143 ,  C12Q2545/114 ,  C12Q2561/113

摘要： The present methods pertain to amplifying and/or detecting Staphylococcus aureus (“SA”) and methicillin-resistant Staphylococcus aureus (“MRSA”) nucleic acids based on a combined detection of ldh1 as a SA marker and mecA as a MRSA marker. In certain embodiments the methods also pertain to amplifying and/or detecting one or more SCCmec integration sites or bridge regions. Primers and probes are suitable to be used in the present methods to detect SA and MRSA simultaneously in a single reaction or in separate reactions. The amplified nucleic acid can be detected by a variety of state of the art methods, including fluorescence resonance energy transfer (“FRET”), radiolabels, enzyme labels, and the like.