公开(公告)号：US20200291448A1

公开(公告)日：2020-09-17

申请号：US16885323

申请日：2020-05-28

申请人： ENZO LIFE SCIENCES, INC.

发明人： Irina LEBEDEVA ,  Wayne Forest PATTON ,  Maciej SZCZEPANIK ,  Yuejun XIANG ,  Praveen PANDE

IPC分类号： C12Q1/26 ,  C09K11/06 ,  C12Q1/37 ,  C12Q1/44 ,  C12Q1/48 ,  C07D311/08 ,  C07D491/052 ,  C07D491/20 ,  C07D493/10

摘要： Provided is a compound having the structure: (SIG)-(SI-MOD)m where SIG is a signaling molecule, SI is a self-immolative structure bound to SIG such that SIG has a reduced signal relative to the signal of SIG without SI, MOD is a moiety bound to SI that is subject to modification by an activator, and m is an integer from 1 to about 10. When MOD is modified by an activator, SI is destabilized and self-cleaved from SIG such that SIG generates an increased signal. Also provided are methods of determining whether a sample, such as a cell, comprises an activator, such as a nitroreducase, using the compound. Further provided are methods of determining whether a mammalian cell is hypoxic using the compound where nitroreductase is the activator. A method of detecting a microorganism that comprises a nitroreductase using the compound where nitroreductase is the activator is also provided.

公开(公告)号：US20230175036A1

公开(公告)日：2023-06-08

申请号：US18161610

申请日：2023-01-30

申请人： Enzo Life Sciences, Inc.

发明人： Irina LEBEDEVA ,  Wayne Forrest PATTON ,  Maciej SZCZEPANIK ,  Yuejun XIANG ,  Praveen PANDE

IPC分类号： C12Q1/26 ,  C07C271/08 ,  C09K11/06 ,  C12Q1/37 ,  C12Q1/44 ,  C12Q1/48 ,  C07D311/08 ,  C07D491/052 ,  C07D491/20 ,  C07D493/10

CPC分类号： C12Q1/26 ,  C07C271/08 ,  C09K11/06 ,  C12Q1/37 ,  C12Q1/44 ,  C12Q1/485 ,  C07D311/08 ,  C07D491/052 ,  C07D491/20 ,  C07D493/10 ,  Y10T436/18 ,  G01N2333/902 ,  G01N2500/00 ,  G01N2333/92 ,  G01N2333/918 ,  C09K2211/1022 ,  C09K2211/1088

摘要： Provided is a compound comprising the structure:


(SIG)-(SI-MOD)m.

In this compound, SIG is a signaling molecule, SI is a self-immolative structure bound to SIG such that SIG has a reduced signal relative to the signal of SIG without SI, MOD is a moiety bound to SI that is subject to modification by an activator, and m is an integer from 1 to about 10. With this compound, when MOD is modified by an activator, SI is destabilized and self-cleaved from SIG such that SIG generates an increased signal. Also provided is a method of determining whether a sample comprises an activator, using the above-described compound. Additionally provided is a method of determining whether a cell comprises a nitroreductase using the above-described compound where nitroreductase is the activator. Further provided is a method of determining whether a mammalian cell is hypoxic using the above-described compound where nitroreductase is the activator. A method of detecting a microorganism that comprises a nitroreductase, using the above-described compound where nitroreductase is the activator, is also provided. Also provided is a method of identifying nitroreductase in a sample, using the above-described compound where nitroreductase is the activator.