公开(公告)号：US20240210301A1

公开(公告)日：2024-06-27

申请号：US18288218

申请日：2022-04-22

申请人： ETH Zurich

发明人： Andrew De Mello ,  Stavros Stavrakis ,  Xiaobao Cao ,  Mohammad Asghari ,  Mahmut Aslan ,  Bogdan Mateescu ,  Yingchao Meng

IPC分类号： G01N15/1404 ,  B01L3/00 ,  G01N15/14 ,  G01N15/1433

CPC分类号： G01N15/1404 ,  B01L3/502761 ,  G01N15/1433 ,  G01N15/1484 ,  B01L2200/0636 ,  G01N2015/1495

摘要： In a method of investigating cell deformations, a sample fluid including cells suspended in a suspending medium is provided. A flow of the sample fluid through a focusing microchannel is established. The suspending medium is a non-Newtonian fluid having viscoelastic properties such that cells that enter the focusing microchannel are focused towards a center of the focusing microchannel due to the viscoelastic properties of the suspending medium, causing the cells to exit the focusing microchannel in single file. Subsequently, the sample fluid that has exited the focusing microchannel is caused to flow through a deformation microchannel arranged downstream of the focusing microchannel to cause a deformation of cells that have exited the focusing microchannel and have entered the deformation microchannel, the deformation being caused by a flow pattern created by interaction of the fluid flow with the deformation microchannel.