公开(公告)号：US20240353309A1

公开(公告)日：2024-10-24

申请号：US18734985

申请日：2024-06-05

申请人： BECTON, DICKINSON AND COMPANY

发明人： Eric D. Diebold ,  Keegan Owsley ,  Jonathan Lin

IPC分类号： G01N15/1434 ,  G01N15/10 ,  G01N15/14 ,  G01N15/1429 ,  G01N15/1433 ,  G01N21/64 ,  G02B21/06

CPC分类号： G01N15/1434 ,  G01N15/14 ,  G01N15/1425 ,  G01N15/1429 ,  G01N15/1433 ,  G01N15/147 ,  G01N21/645 ,  G02B21/06 ,  G01N2015/1006 ,  G01N2015/1447 ,  G01N2015/1454 ,  G01N2021/6482

摘要： In one aspect, the present teachings provide a system for performing cytometry that can be operated in three operational modes. In one operational mode, a fluorescence image of a sample is obtained by exciting one or more fluorophore(s) present in the sample by an excitation beam formed as a superposition of a top-hat-shaped beam with a plurality of beams that are radiofrequency shifted relative to one another. In another operational mode, a sample can be illuminated successively over a time interval by a laser beam at a plurality of excitation frequencies in a scanning fashion. The fluorescence emission from the sample can be detected and analyzed, e.g., to generate a fluorescence image of the sample. In yet another operational mode, the system can be operated to illuminate a plurality of locations of a sample concurrently by a single excitation frequency, which can be generated, e.g., by shifting the central frequency of a laser beam by a radiofrequency. For example, a horizontal extent of the sample can be illuminated by a laser beam at a single excitation frequency. The detected fluorescence radiation can be used to analyze the fluorescence content of the sample, e.g., a cell/particle.

公开(公告)号：US20240344959A1

公开(公告)日：2024-10-17

申请号：US18291952

申请日：2022-07-29

申请人： TECHNISCHE UNIVERSITÄT MÜNCHEN

发明人： Oliver HAYDEN ,  Johanna ERBER ,  Sebastian RASCH ,  Tobias LAHMER ,  Stefan RÖHRL ,  Christian KLENK

IPC分类号： G01N15/1404 ,  G01N15/01 ,  G01N15/1433

CPC分类号： G01N15/1404 ,  G01N15/01 ,  G01N15/1433 ,  G01N2015/016 ,  G01N2015/018 ,  G01N2015/1413

摘要： Disclosed herein is a method for detecting cell aggregates of biological cells using a quantitative phase-contrast microscope and a device for detecting cell aggregates of biological cells using said method. The method comprises preparing a suspension comprising biological cells from a sample. A flow of the suspension is generated along a microfluidic channel to viscoelastically and/or hydrodynamically focus cell aggregates in the suspension in a focal plane of the quantitative phase-contrast microscope. One or more phase shift images of the biological cells in the suspension are taken using the quantitative phase-contrast microscope. Cell aggregates in the one or more phase shift images are identified. The sample is a whole blood sample or a blood fraction sample and identifying cell aggregates in the one or more phase shift images comprises identifying platelet aggregates in the one or more phase shift images.

公开(公告)号：US12111244B2

公开(公告)日：2024-10-08

申请号：US17523989

申请日：2021-11-11

申请人： HON HAI PRECISION INDUSTRY CO., LTD.

发明人： Wan-Jhen Lee ,  Chin-Pin Kuo ,  Chih-Te Lu

IPC分类号： G06K9/00 ,  G01N15/1433 ,  G01N33/483 ,  G06T7/00

CPC分类号： G01N15/1433 ,  G01N33/4833 ,  G06T7/0012 ,  G06T2207/30024 ,  G06T2207/30242

摘要： A method for calculating a density of stem cells in a cell image and an electronic device are provided. A plurality of preset ratios and a plurality of density calculation models can be used to perform hierarchical density calculations on the cell image. Starting from the largest preset ratio (the first preset ratio) reduction of the cell image to no reduction, the density calculation is performed on the cell image using a model starting with a highest density calculation (the first density calculation model) to a model with the smallest density calculation (the third density calculation model), which can quickly detect densities of various stem cells. Using different preset ratios and corresponding density calculation models for calculation, it is not necessary to calculate the number of stem cells to obtain the density of stem cells, which improves a calculation efficiency of the density of stem cells.

公开(公告)号：US20240309308A1

公开(公告)日：2024-09-19

申请号：US18430569

申请日：2024-02-01

申请人： Thrive Bioscience, Inc.

发明人： Alan Blanchard

IPC分类号： C12M1/00 ,  C12M1/34 ,  C12M1/36 ,  G01N15/10 ,  G01N15/1433 ,  G01N15/1434 ,  G01N35/00 ,  G01N35/04

CPC分类号： C12M41/14 ,  C12M41/36 ,  C12M41/40 ,  C12M41/48 ,  G01N15/1433 ,  G01N35/00732 ,  G01N35/0099 ,  G01N2015/1006 ,  G01N2015/1454 ,  G01N2035/00356 ,  G01N2035/0463

摘要： Aspects of the invention relate to automated cell culture incubators. In some embodiments, automated cell culture incubators comprise an integrated manipulation device having one or more cell scrapers.

公开(公告)号：US20240302265A1

公开(公告)日：2024-09-12

申请号：US18570603

申请日：2022-06-16

申请人： THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

发明人： Yu-Hwa Lo ,  Xinyu Chen ,  Lauren Waller ,  Jiajie Chen

IPC分类号： G01N15/149 ,  G01N15/10 ,  G01N15/1433 ,  G01N15/1434

CPC分类号： G01N15/149 ,  G01N15/1433 ,  G01N15/1434 ,  G01N2015/1006

摘要： Disclosed are devices, systems and methods for label-free, image-encoded, microfluidics-based cell sorting. In some aspects, an image-based particle sorting system includes an optical imaging system; a data processing system to process the image data obtained by the optical imaging device and determine one or more properties associated with the individual particles flowing in the carrier fluid and to produce a control command based on a comparison of the determined one or more properties with a sorting criteria; and a particle sorting system including a particle flow device that comprises a substrate including the particle-flow channel and a plurality of output channels branching from the particle-flow channel to receive, in one output channel of the plurality of output channels, sorted particles directed by an actuator device based on the control command.

公开(公告)号：US20240293817A1

公开(公告)日：2024-09-05

申请号：US18655434

申请日：2024-05-06

申请人： Xilis, Inc.

发明人： Bradley Scott Thomas ,  Timothy A. Miller ,  David Stafford

IPC分类号： B01L3/00 ,  C12M1/00 ,  C12M1/12 ,  C12M1/34 ,  C12M3/06 ,  G01N15/14 ,  G01N15/1433 ,  G01N15/1434

CPC分类号： B01L3/502784 ,  B01L3/50273 ,  B01L3/502746 ,  C12M23/16 ,  C12M23/40 ,  C12M25/16 ,  C12M41/12 ,  C12M41/36 ,  C12M41/40 ,  G01N15/1433 ,  G01N15/1434 ,  G01N15/1459 ,  B01L2200/061 ,  B01L2200/0673 ,  B01L2300/0654 ,  B01L2300/0883 ,  B01L2300/14 ,  B01L2300/161 ,  B01L2300/1805 ,  B01L2400/082 ,  G01N2015/1493

摘要： A method includes flowing a first fluid through a first channel of a microfluidic apparatus and flowing a second fluid through a second channel of the microfluidic apparatus. The first fluid comprises biological material and a matrix material and is immiscible with the second fluid. The first and second fluids are combined at a junction to form droplets of the first fluid dispersed in the second fluid in a third channel. Multiple exposures of a droplet in the third channel are captured in a single image, comprising: illuminating a region of the third channel with multiple successive illumination pulses during a single frame of the imaging device; identifying the droplet and determining a velocity or a size of the droplet based on an analysis of the captured exposures; and controlling the flow of the first fluid or second fluid to obtain droplets of a target size or velocity.

公开(公告)号：US12076725B2

公开(公告)日：2024-09-03

申请号：US17244199

申请日：2021-04-29

申请人： Illumina, Inc.

发明人： Darryl Donovan

IPC分类号： B01L9/00 ,  B01L3/00 ,  B01L7/00 ,  G01N15/10 ,  G01N15/14 ,  G01N15/1433 ,  G01N21/03 ,  G01N21/64

CPC分类号： B01L9/52 ,  B01L3/502715 ,  G01N15/1433 ,  G01N15/1484 ,  G01N21/0332 ,  G01N21/6428 ,  B01L7/00 ,  B01L2200/025 ,  B01L2200/0647 ,  B01L2200/0684 ,  B01L2200/0689 ,  B01L2200/147 ,  B01L2300/023 ,  B01L2300/043 ,  B01L2300/0636 ,  B01L2300/0809 ,  B01L2300/0822 ,  B01L2300/0877 ,  B01L2300/0887 ,  B01L2300/1844 ,  B01L2300/185 ,  B01L2400/0406 ,  B01L2400/0457 ,  G01N2015/1006 ,  G01N2021/6441

摘要： Fluidic device includes a manifold body having first and second body sides. The first body side has receiving ports forming a port array that defines a reaction region. The second body side has open-sided recesses forming reaction chambers when the fluidic device is mounted onto a sample substrate. The reaction chambers form a chamber array that defines a fluid-delivery region. The reaction region is greater than the fluid-delivery region. The manifold body also includes vent openings that open to an exterior. The fluidic device also includes upstream channels extending through the manifold body. Each of the upstream channels fluidly couples a corresponding receiving port of the port array to a corresponding reaction chamber of the chamber array. The fluidic device also includes venting channels extending through the manifold body. Each of the venting channels fluidly couples a corresponding reaction chamber of the chamber array to a corresponding vent opening.

公开(公告)号：US12032147B2

公开(公告)日：2024-07-09

申请号：US17255808

申请日：2018-10-01

申请人： Hewlett-Packard Development Company, L.P.

发明人： Fausto D'Apuzzo ,  Viktor Shkolnikov

IPC分类号： G02B21/12 ,  G01N15/0227 ,  G01N15/10 ,  G01N15/14 ,  G01N15/1433 ,  G01N15/1434 ,  G02B21/00 ,  G02B21/36

CPC分类号： G02B21/12 ,  G01N15/0227 ,  G01N15/1433 ,  G01N15/1434 ,  G01N15/1459 ,  G02B21/0004 ,  G02B21/36 ,  G01N2015/1006 ,  G01N2015/1493

摘要： A system and a method for imaging microfluidic ejectors during operation. An example provides a microscopy system, that includes a plurality of microfluidic ejectors. A mirror is disposed in a droplet path of the plurality of microfluidic ejectors, wherein the droplet path passes through an opening in the mirror. An optical system is focused on the plurality of microfluidic ejectors through the mirror, wherein the optical system comprises a camera.

公开(公告)号：US12031897B2

公开(公告)日：2024-07-09

申请号：US17598648

申请日：2020-03-30

申请人： Memorial Sloan Kettering Cancer Center

发明人： Alexandros Pertsinidis

IPC分类号： G01N15/1433 ,  G01N21/64 ,  G02B21/00 ,  G01N15/00

CPC分类号： G01N15/1433 ,  G01N21/6458 ,  G02B21/0076 ,  G01N2015/0038 ,  G01N21/64 ,  G01N2021/6478

摘要： A target may be tagged, and its position tracked to maintain the position of the target at the center (e.g., zero region) of a suppression beam, where suppression is minimal, so the signal from the target is not suppressed relative to signal from background molecules. Point scanning is not needed, as a pixelated detector of a camera can be used to acquire the position of the target in one shot. Images of the object from the pixelated detector can be analyzed to acquire the position of the target. To maintain the target in the zero, the beams may be steered onto the target. Alternatively or additionally, the sample may be moved to place the sample in the center. A correction signal may be sent to cause the sample and/or beams to be moved to maintain the target in position.

公开(公告)号：US12031895B2

公开(公告)日：2024-07-09

申请号：US17081585

申请日：2020-10-27

申请人： OP-Hygiene IP GmbH

发明人： Siegfried Steltenkamp ,  Heiner Ophardt ,  Albrecht Lang

IPC分类号： G01N15/1031 ,  B01L3/00 ,  G01N15/10 ,  G01N15/14 ,  G01N15/1429 ,  G01N15/1433

CPC分类号： G01N15/1031 ,  B01L3/502715 ,  B01L3/502761 ,  G01N15/1023 ,  G01N15/14 ,  G01N15/1429 ,  G01N15/1433 ,  B01L2200/0647 ,  B01L2200/0652 ,  B01L2300/0645 ,  B01L2300/0681 ,  B01L2300/087 ,  G01N2015/1006 ,  G01N2015/1024

摘要： A method of analysing and identifying particles in an input fluid by electrical field analysis preferably in combination with imaging analysis and towards establishing characterises of the particles that can be compared with characteristics of known particles, preferably biologic particles such as bacteria and viruses. Preferably in a focusing step, the particles are focused as in a microfluidic particle sorting cartridge followed by electrical field analysis to determine impedance data. Preferably, the electrical field analysis is carried out in a water and alcohol solution.