公开(公告)号：US12092560B2

公开(公告)日：2024-09-17

申请号：US17536437

申请日：2021-11-29

申请人： SYSMEX CORPORATION

发明人： Masatsugu Ozasa ,  Daiki Fujiwara ,  Daiki Mita ,  Takaaki Okajima ,  Hideki Hirayama

IPC分类号： G01N15/1434 ,  G01N15/1404 ,  G01N35/02

CPC分类号： G01N15/1436 ,  G01N15/1404 ,  G01N35/025

摘要： A detection method according to one or more embodiments may include transporting a rack holding a reaction vessel along a first axis toward a sample dispensing position, linearly moving the sample dispensing pipette above the rack along a second axis intersecting the first axis and dispensing a sample into the reaction vessel located at the sample dispensing position, transporting the rack along the first axis toward a reagent dispensing position; linearly moving a reagent dispensing pipette above the rack along a third axis intersecting the first axis, and dispensing a reagent into the reaction vessel located at the reagent dispensing position, and detecting a detection target in a measurement specimen prepared from the sample and the reagent.

公开(公告)号：US20240293815A1

公开(公告)日：2024-09-05

申请号：US17617545

申请日：2021-10-18

申请人： FUDAN UNIVERSITY

发明人： Antoine Jean-Pierre Rene RIAUD ,  Qing WANG ,  Jia ZHOU

IPC分类号： B01L3/00 ,  G01N15/14 ,  G01N15/1404 ,  G01N15/1409

CPC分类号： B01L3/502761 ,  B01L3/50273 ,  G01N15/1404 ,  G01N15/1409 ,  G01N15/1425 ,  B01L2200/0668 ,  B01L2400/0436 ,  G01N2015/1415

摘要： The invention discloses a method and an apparatus for trapping and manipulating particles, relating to the field of acoustic manipulation. The invention discloses a photoacoustic tweezers, which use bimodal transducer generate a weak acoustic wave and a strong acoustic wave. The strong acoustic wave behaves as an effective gain medium to amplify the acoustic radiation force of this weak acoustic wave. After an interference of the strong acoustic wave and the weak acoustic wave, an amplified trapping force is produced for achieving the trapping and manipulation of particles. The invention can manipulate several particles simultaneously in high throughput and particles of various sizes ranging from 1 μm to 1 mm. The invention can form reconfigurable acoustic fields which permits versatile and selective manipulation with no need of complicated acoustic arrays. The invention is a versatile, biocompatible, selective and high-throughput manipulation method.

公开(公告)号：US12072277B2

公开(公告)日：2024-08-27

申请号：US17084683

申请日：2020-10-30

申请人： Government of the United States of America, as represented by the Secretary of Commerce

发明人： Gregory Alan Cooksey ,  Paul Nathan Patrone ,  Anthony Jose Kearsley

IPC分类号： G01N15/1434 ,  G01N15/1404

CPC分类号： G01N15/1436 ,  G01N15/1404

摘要： A multiplexed amplitude modulation photometer includes a microchannel; a first input light path that: receives a first modulated light at a first modulation frequency; and communicates the first modulated light to a first optical region that receives the first analyte that produces a first output light including the first modulation frequency communicated to a first detection light path; the first optical region; the first detection light path that receives the first output light; a second input light path that: receives a second modulated light with a second modulation frequency; and communicates second modulated light to a second optical region that receives the second analyte that produces a second output light with the second modulation frequency communicated to a second detection light path; the second optical region; and the second detection light path that receives the second output light from the second optical region.

公开(公告)号：US12044626B2

公开(公告)日：2024-07-23

申请号：US17857817

申请日：2022-07-05

申请人： ILLUMINA, INC.

发明人： Wenyi Feng ,  Simon Prince ,  Peter Clarke Newman ,  Dakota Watson ,  Stanley S. Hong ,  Marco A. Krumbuegel ,  Yinghua Sun ,  Andrew James Carson ,  Merek C. Siu

IPC分类号： G01N21/85 ,  C12Q1/6869 ,  G01N15/1404 ,  G01N21/64 ,  G02B7/00 ,  G01N15/01 ,  G01N15/10 ,  G01N15/1434

CPC分类号： G01N21/85 ,  C12Q1/6869 ,  G01N15/1404 ,  G01N21/6458 ,  G02B7/003 ,  G01N15/01 ,  G01N2015/1006 ,  G01N2015/1452 ,  G01N2201/024 ,  C12Q1/6869 ,  C12Q2565/601

摘要： A system includes a plurality of modular subassemblies and a plate; wherein each modular subassembly comprises an enclosure and a plurality of optical components aligned to the enclosure, and each enclosure comprises a plurality of mounting structures; and wherein each modular subassembly is mechanically coupled to the plate by attachment of a mounting structure of the modular subassembly directly to a corresponding mounting structure located on the plate, such that by mechanically coupling each modular subassembly to the plate using the mounting structure of the modular subassembly and the corresponding mounting structure on the plate, adjacent modular subassemblies are aligned to each other upon such attachment, and wherein two of the modular subassemblies mechanically coupled to the plate are also attached to each other by mechanically coupling an alignment structure on one of the two modular subassemblies to a respective alignment structure on the other of the two modular subassemblies.

公开(公告)号：US11982612B2

公开(公告)日：2024-05-14

申请号：US17724462

申请日：2022-04-19

申请人： BaySpec, Inc.

发明人： Kotaro Hiramatsu ,  Ryo Nishiyama ,  Keisuke Goda ,  Kosuke Dodo ,  Shintaro Kawamura ,  Mikiko Sodeoka ,  Hideyusi Suzuki ,  Charlie Zhang ,  William Yang Terziyan

IPC分类号： G01N15/1434 ,  G01N15/1404 ,  G01N21/65 ,  G01N15/10

CPC分类号： G01N15/1434 ,  G01N15/1404 ,  G01N21/65 ,  G01N2015/1006 ,  G01N2015/1438

摘要： Systems and methods for flow cytometry. The methods comprise: labeling cells of a sample with Raman tags; causing the sample to flow through a microfluidic channel of a flow cytometer through which a laser beam passes; detecting Raman signals emitted from the Raman tags while being illuminated by the laser beam; and determining characteristics of the cells based on the detected Raman signals.

公开(公告)号：US20240151630A1

公开(公告)日：2024-05-09

申请号：US18393982

申请日：2023-12-22

申请人： Sony Group Corporation

发明人： Fumitaka Otsuka

IPC分类号： G01N15/1404 ,  G01N15/1429

CPC分类号： G01N15/1404 ,  G01N15/1429 ,  G01N2015/142 ,  G01N15/149

摘要： To provide a droplet forming technology capable of stably sorting a droplet.
Provided is a microparticle sorting device provided with a voltage supply unit that supplies a drive voltage to a vibration element that applies a vibration to an orifice that generates a fluid stream, a control unit that controls a driving condition supplied to the vibration element on the basis of a relative relationship between a droplet discharged from the orifice and a satellite droplet present between droplets, and a sorting unit that sorts the droplet containing microparticles on the basis of optical information detected from the microparticles flowing through a flow path.

公开(公告)号：US20240142460A1

公开(公告)日：2024-05-02

申请号：US18276666

申请日：2022-02-11

申请人： YISSUM RESEARCH DEVELOPMENT COMPANY OF THE HEBREW UNIVERSITY OF JERUSALEM LTD. ,  LUDWIG-MAXIMILIANS-UNIVERSITÄT MÜNCHEN

发明人： Eitan LERNER ,  Thorben CORDES ,  Yair RAZVAG ,  Paz DRORI ,  Gabriel MOYA

IPC分类号： G01N33/58 ,  G01N15/14 ,  G01N15/1404

CPC分类号： G01N33/582 ,  G01N15/1404 ,  G01N15/1484

摘要： Systems and methods that allow optical detection of nanoparticles according to size and interaction with specific antibodies. Using abiological sample combined with fluorescent dyes and fluorescently labeled antibodies, implementations of the disclosed system allow for detection of >100 particles having diameters as low as 100 nm in under 10 minutes. The detection mechanisms combine confocal detection of particles in microfluidic flow devices. Concentrating the sample using hydrodynamic focusing allows detection of particles having concentrations as low as 104 particles per mL of the sample. These capabilities allow for the detection, identification, and quantitation of viruses from bodily fluids such as saliva, where biologically relevant virus concentrations of potentially infected subjects are within the range of 103-107 particles/mL. The system and methods comprise a ‘flow virometer’ providing rapid, direct feedback regarding the existence of specific viruses in a biological sample and serves as a technological basis for commercial products.

公开(公告)号：US11971342B2

公开(公告)日：2024-04-30

申请号：US17543415

申请日：2021-12-06

申请人： SYSMEX CORPORATION

发明人： Takaaki Nagai ,  Shinichiro Oguni ,  Tomohiro Tsuji

IPC分类号： G01N15/14 ,  G01N1/38 ,  G01N1/40 ,  G01N15/10 ,  G01N15/1404 ,  G01N15/1434 ,  G01N35/00 ,  G01N35/10

CPC分类号： G01N15/1404 ,  G01N1/38 ,  G01N1/4077 ,  G01N15/1436 ,  G01N15/1459 ,  G01N35/1002 ,  G01N2001/386 ,  G01N2001/4083 ,  G01N2015/1006 ,  G01N2035/00495

摘要： The sample preparation apparatus includes a measurement unit configured to measure a sample containing particles acquired from a sample container and detect measurement target particles in the sample, a sample preparation unit capable of adjusting the concentration of measurement target particles in a sample acquired from a sample container and configured to prepare a measurement sample by mixing a sample and any of a plurality of types of particle detection reagents including a particle labeling substance, and a control unit for controlling the sample preparation unit so as to generate concentration information of the measurement target particles in the sample in the sample container based on the measurement data of the measurement unit and adjust the concentration of the measurement target particles in the sample acquired from the sample container according to the generated concentration information and the type of particle detection reagent used for preparing the measurement sample.

公开(公告)号：US20240361228A1

公开(公告)日：2024-10-31

申请号：US18768428

申请日：2024-07-10

申请人： Singular Genomics Systems, Inc.

发明人： Sandor Kovacs ,  Anson Hsu ,  David Baranson ,  John Van Doorn ,  Eli N. Glezer

IPC分类号： G01N15/1404 ,  G01N15/01 ,  G01N15/14

CPC分类号： G01N15/1404 ,  G01N15/147 ,  G01N2015/014 ,  G01N2015/1402 ,  G01N2015/1497

摘要： The present disclosure relates to a flow cell carrier. The flow cell carrier may include a flow cell and a frame. The frame may include a pocket and a handle. The pocket may have at least one spring feature and at least one banking feature. The frame may be configured to retain the flow cell within the pocket such that a maximal surface area of the flow cell is exposed to an optical lens. Related methods and kits are also disclosed.

公开(公告)号：US12105007B2

公开(公告)日：2024-10-01

申请号：US16333528

申请日：2017-09-15

申请人： ON-CHIP BIOTECHNOLOGIES CO., LTD.

发明人： Kazuo Takeda ,  Yuu Fujimura ,  Masayuki Ishige ,  Takahide Ino ,  Yohsuke Bansho ,  Jin Akagi ,  Kosuke Osawa

IPC分类号： G01N15/1404 ,  B01L3/02 ,  G01N15/10 ,  G01N15/14 ,  G01N15/1433 ,  G01N15/1434 ,  G01N15/149 ,  C12Q1/68 ,  C12Q1/686

CPC分类号： G01N15/1404 ,  B01L3/021 ,  G01N15/1433 ,  B01L2200/0636

摘要： The object of the present invention is to provide a technique for dispensing cells as single cells. The above problem can be solved by an apparatus for dispensing particles comprising a transparent hollow pipette for dispensing sample liquid containing particles, and an image capturing means, wherein the devise has means for capturing two or more images of the dispensing liquid in the hollow pipette; means for comparing two or more shot images, distinguishing a moved particle-like substance from an unmoved particle-like substance in the image-captured particle-like substances, and identifying the moved particle-like substance as a suspended particle; and means for dispensing a sample liquid containing a target number of suspended particles.