公开(公告)号：US20210171934A1

公开(公告)日：2021-06-10

申请号：US16893966

申请日：2020-06-05

Applicant: Exosome Diagnostics, Inc.

Inventor： Daniel ENDERLE ,  Aparna RAMACHANDRAN ,  Haoheng YAN ,  Emily BERGHOFF ,  Tai-Fen WEI ,  Mikkel NOERHOLM ,  Johan Karl Olov SKOG

IPC: C12N15/10 ,  C12Q1/6806

Abstract: The invention provides novel methods for isolating microvesicles from a biological sample and for extracting nucleic acids from the microvesicles.

公开(公告)号：US20190093172A1

公开(公告)日：2019-03-28

申请号：US16092358

申请日：2017-04-17

Applicant: Exosome Diagnostics, Inc.

Inventor： Johan Karl Olov SKOG ,  Mikkel NOERHOLM ,  James HURLEY ,  Elena CASTELLANOS-RIZALDOS ,  Kay BRINKMAN

IPC: C12Q1/6886 ,  C12N15/10 ,  C12Q1/686 ,  G16H50/20

Abstract: The present invention relates generally to the field of biomarker analysis, particularly determining gene expression signatures from biological samples, including plasma samples.

公开(公告)号：US20210403881A1

公开(公告)日：2021-12-30

申请号：US17295287

申请日：2019-11-20

Applicant: Exosome Diagnostics, Inc.

Inventor： Georg STOLL ,  Daniel ENDERLE ,  Mikkel NOERHOLM ,  Johan Karl Olov SKOG

IPC: C12N7/00 ,  C12Q1/70 ,  C12N15/10

Abstract: The present disclosure provides compositions, methods and kits for internal controls of microvesicle isolations. The compositions, methods and kits can comprise enveloped viruses, including, but not limited to, inactive mouse hepatitis virus (MHV).

公开(公告)号：US20210388453A1

公开(公告)日：2021-12-16

申请号：US17411606

申请日：2021-08-25

Applicant: Exosome Diagnostics, Inc.

Inventor： Mikkel NOERHOLM ,  Susan BELZER ,  Charlotte ROMAIN ,  Johan Karl Olov SKOG ,  Leileata M. RUSSO ,  Wayne COMPER

IPC: C12Q1/6886 ,  C12N7/00

Abstract: The present invention features a method and kit for isolating microvesicles or extracting microvesicle nucleic acids from a biological sample by using a control particle. The present invention provides control particles that are viruses or virus-like particles, such as bacteriophages, that contain control nucleic acids that can be detected to assess the accuracy, reliability, and efficiency of the microvesicle isolation or nucleic acid extraction steps. The methods described herein may further comprise the analysis of the presence, absence, or level of at least one biomarker associated with a disease or medical condition for diagnosing, prognosing, or monitoring the disease or medical condition.

公开(公告)号：US20190284548A1

公开(公告)日：2019-09-19

申请号：US16301176

申请日：2017-05-15

Applicant: Exosome Diagnostics, Inc.

Inventor： Georg STOLL ,  Daniel ENDERLE ,  Mikkel NOERHOLM ,  Johan Karl Olov SKOG

IPC: C12N15/10 ,  C12N15/11

Abstract: The invention provides novel methods and kits for fully automated high-throughput method for isolation of extracellular vesicles and co-isolation of cell-free DNA from biofluids, including cell-free DNA and/or cell-free DNA and nucleic acids including at least RNA from microvesicles, novel methods and kits for isolation of extracellular vesicles and co-isolation of cell-free DNA from biofluids, including cell-free DNA and/or cell-free DNA and nucleic acids including at least RNA from microvesicles that do not require the use of phenol or chloroform, and for extracting nucleic acids from the extracellular vesicles and/or from the biological samples.

公开(公告)号：US20160312211A1

公开(公告)日：2016-10-27

申请号：US14424694

申请日：2013-08-30

Applicant: Exosome Diagnostics, Inc.

Inventor： Mikkel NOERHOLM

IPC: C12N15/10 ,  C12Q1/68 ,  C12N7/00

CPC classification number: C12Q1/6886 ,  C12N7/00 ,  C12N2795/18122 ,  C12N2795/18131 ,  C12Q2600/106 ,  C12Q2600/118

Abstract: The present invention features a method and kit for isolating microvesicles or extracting microvesicle nucleic acids from a biological sample by using a control particle. The present invention provides control particles that are viruses or virus-like particles, such as bacteriophages, that contain control nucleic acids that can be detected to assess the accuracy, reliability, and efficiency of the microvesicle isolation or nucleic acid extraction steps. The methods described herein may further comprise the analysis of the presence, absence, or level of at least one biomarker associated with a disease or medical condition for diagnosing, prognosing, or monitoring the disease or medical condition.

Abstract translation: 本发明的特征在于通过使用对照颗粒从生物样品中分离微泡或提取微囊泡核酸的方法和试剂盒。 本发明提供了控制颗粒，其是包含可以检测以评估微泡分离或核酸提取步骤的准确性，可靠性和效率的对照核酸的病毒或病毒样颗粒，例如噬菌体。 本文所述的方法还可以包括与用于诊断，预后或监测疾病或医疗状况的疾病或医学状况相关联的至少一种生物标志物的存在，不存在或水平的分析。

公开(公告)号：US20200149036A1

公开(公告)日：2020-05-14

申请号：US16473604

申请日：2018-01-02

Applicant: Exosome Diagnostics, Inc.

Inventor： Daniel ENDERLE ,  Johan SKOG ,  Phillipp TORKLER ,  Alexandra SPIEL ,  Mikkel NOERHOLM

IPC: C12N15/10 ,  C12Q1/6806 ,  C12Q1/6874

Abstract: The invention provides a series of steps to distinguish RNA from DNA in assays downstream of a combined isolation. This enables a wide diversity of RNAs and/or DNAs, to be efficiently detected.

公开(公告)号：US20200056244A1

公开(公告)日：2020-02-20

申请号：US16375237

申请日：2019-04-04

Applicant: Exosome Diagnostics, Inc.

Inventor： Johan Karl Olov SKOG ,  Mikkel NOERHOLM

IPC: C12Q1/6886

Abstract: The present invention relates generally to the field of biomarker analysis, particularly determining gene expression signatures from urine samples. The disclosure provides compositions, kits and methods for diagnosing a prostate disorder such as prostate cancer in a male subject.

公开(公告)号：US20220355290A1

公开(公告)日：2022-11-10

申请号：US17621084

申请日：2020-06-24

Applicant: Exosome Diagnostics, Inc.

Inventor： Georg STOLL ,  Frank NUSS ,  Daniel ENDERLE ,  Mikkel NOERHOLM

IPC: B01L3/00 ,  A61B10/00

Abstract: Described herein are various inventions and embodiments thereof, directed to systems, devices, and methods for separating a sample. Embodiments of sample collection devices disclosed herein may maintain separation of different volumes of sample during collecting, handling, and accessing of the sample. A sample collection device, in some embodiments, may comprise an enclosure defining a first chamber, a second chamber, and a partition therebetween configured to separate the first chamber and the second chamber. An assembly coupled to the partition may be configured to transition between an open configuration and a closed configuration based on fluid and gas flow through the assembly.

公开(公告)号：US20210238582A1

公开(公告)日：2021-08-05

申请号：US17074040

申请日：2020-10-19

Applicant: Exosome Diagnostics, Inc.

Inventor： Georg STOLL ,  Daniel ENDERLE ,  Mikkel NOERHOLM ,  Johan Karl Olov SKOG

IPC: C12N15/10 ,  C12N15/11

Abstract: The invention provides novel methods and kits for fully automated high-throughput method for isolation of extracellular vesicles and co-isolation of cell-free DNA from biofluids, including cell-free DNA and/or cell-free DNA and nucleic acids including at least RNA from microvesicles, novel methods and kits for isolation of extracellular vesicles and co-isolation of cell-free DNA from biofluids, including cell-free DNA and/or cell-free DNA and nucleic acids including at least RNA from microvesicles that do not require the use of phenol or chloroform, and for extracting nucleic acids from the extracellular vesicles and/or from the biological samples.