公开(公告)号：US20050250176A1

公开(公告)日：2005-11-10

申请号：US11115294

申请日：2005-04-27

申请人： Eiji Kono ,  Hironobu Sekine ,  Yasuhiko Yokoi ,  Akifumi Iwama

发明人： Eiji Kono ,  Hironobu Sekine ,  Yasuhiko Yokoi ,  Akifumi Iwama

IPC分类号： C12M1/34 ,  C12Q1/04 ,  C12Q1/68

CPC分类号： C12Q1/04

摘要： The present invention has an object to provide a simple detecting device and a simple detecting method, to shorten the time for detection, and to improve sensitivity of detection, particularly for gram negative bacteria. The microorganisms detecting device includes a sampling section for taking a sample from a detection target, a sample concentrating section for concentrating microorganisms in the sample, a reagent adding section for adding a reagent which changes the reagent color tone by existence of the microorganisms to the sample, and a microorganisms detecting section for detecting the change of the color tone of the regent by means of an optical system, thereby shortening the time for detection and improving the sensitivity of detection by concentrating the microorganisms in the sample.

摘要翻译： 本发明的目的是提供简单的检测装置和简单的检测方法，缩短检测时间，提高检测灵敏度，特别是革兰氏阴性细菌。 微生物检测装置包括：用于从检测对象取样的取样部，将样品中的微生物浓缩的样品浓缩部，向样品添加使微生物存在而使试剂色调变化的试剂的试剂添加部 以及微生物检测部，用于通过光学系统检测摄氏度的色调变化，从而缩短检测时间，并通过将微生物浓缩在样品中来提高检测灵敏度。

公开(公告)号：US07923242B2

公开(公告)日：2011-04-12

申请号：US11346564

申请日：2006-02-03

申请人： Eiji Kono ,  Hironobu Sekine ,  Yasuhiko Yokoi ,  Akihumi Iwama

发明人： Eiji Kono ,  Hironobu Sekine ,  Yasuhiko Yokoi ,  Akihumi Iwama

IPC分类号： C12M1/34 ,  C12M3/00

CPC分类号： C12Q1/04 ,  G01N21/0317 ,  G01N21/75 ,  G01N21/78 ,  G01N2021/7783

摘要： The objective of the present invention is to provide a simple microorganism detection apparatus that reduces detection time and that provides improved detection sensitivity, especially for gram-negative bacteria. The microorganism detection apparatus of the present invention includes: a detection target introduction portion, into which a sample and reagents are to be introduced; a detector, for detecting the color tone of the reagent introduced into the detection target introduction portion; a sample holder, for holding the sample to be introduced into the detection target introduction portion; a culture solution holder, for holding a culture solution used for microorganism culturing; and reagent holders, for holding the reagents to be introduced into the detection target introduction portion, wherein the presence/absence of microorganisms in the sample is determined based on the detected color tone.

摘要翻译： 本发明的目的是提供一种简单的微生物检测装置，其减少检测时间并且提供改善的检测灵敏度，特别是革兰氏阴性细菌。 本发明的微生物检测装置包括：检测对象物导入部，将样品和试剂引入其中的检测对象物导入部; 检测器，用于检测引入到检测目标导入部分中的试剂的色调; 样品保持器，用于保持待引入检测对象物导入部的样品; 用于保持用于微生物培养的培养液的培养液保持器; 和试剂保持器，用于保持要引入到检测对象物导入部分中的试剂，其中基于检测到的色调确定样品中存在/不存在微生物。

公开(公告)号：US20060246578A1

公开(公告)日：2006-11-02

申请号：US11346564

申请日：2006-02-03

申请人： Eiji Kono ,  Hironobu Sekine ,  Yasuhiko Yokoi ,  Akihumi Iwama

发明人： Eiji Kono ,  Hironobu Sekine ,  Yasuhiko Yokoi ,  Akihumi Iwama

IPC分类号： C12M1/34

CPC分类号： C12Q1/04 ,  G01N21/0317 ,  G01N21/75 ,  G01N21/78 ,  G01N2021/7783

摘要： The objective of the present invention is to provide a simple microorganism detection apparatus that reduces detection time and that provides improved detection sensitivity, especially for gram-negative bacteria. The microorganism detection apparatus of the present invention includes: a detection target introduction portion, into which a sample and reagents are to be introduced; a detector, for detecting the color tone of the reagent introduced into the detection target introduction portion; a sample holder, for holding the sample to be introduced into the detection target introduction portion; a culture solution holder, for holding a culture solution used for microorganism culturing; and reagent holders, for holding the reagents to be introduced into the detection target introduction portion, wherein the presence/absence of microorganisms in the sample is determined based on the detected color tone.

摘要翻译： 本发明的目的是提供一种简单的微生物检测装置，其减少检测时间并且提供改善的检测灵敏度，特别是革兰氏阴性细菌。 本发明的微生物检测装置包括：检测对象物导入部，将样品和试剂引入其中的检测对象物导入部; 检测器，用于检测引入到检测目标导入部分中的试剂的色调; 样品保持器，用于保持待引入检测对象物导入部的样品; 用于保持用于微生物培养的培养液的培养液保持器; 和试剂保持器，用于保持待引入到检测对象物导入部分中的试剂，其中基于检测到的色调确定样品中存在/不存在微生物。

公开(公告)号：US20100112677A1

公开(公告)日：2010-05-06

申请号：US12593347

申请日：2008-03-27

申请人： Jiro Onishi ,  Yasuhiko Yokoi ,  Akifumi Iwama

发明人： Jiro Onishi ,  Yasuhiko Yokoi ,  Akifumi Iwama

IPC分类号： C12M1/12 ,  A61L2/20 ,  A61L2/22 ,  B01L1/00

CPC分类号： A61L2/202 ,  A61L2/22 ,  A61L2202/13 ,  B01L1/04 ,  B01L2300/0681 ,  B01L2300/10

摘要： Provided is a sterile environment maintaining apparatus capable of sterilizing a sterile room in a shorter time than conventional ones.The sterile environment maintaining apparatus according to the present invention comprises a sterile room 10 having an inlet opening 11 and an outlet opening 12, a HEPA filter 3 placed so as to cover the inlet opening 11 or the inlet opening 11 and the outlet opening 12 of the sterile room 10, and a sterilizing material supplying part supplying a sterilizing material such as hydrogen peroxide to the sterile room 10, and the sterile room 10 is provided with a detoxifying material supplying part spraying a detoxifying material such as ozone toward the HEPA filter 3.

摘要翻译： 本发明提供一种无菌环境保持装置，其能够在比常规方法更短的时间内对无菌室进行灭菌。 根据本发明的无菌环境保持装置包括具有入口开口11和出口开口12的无菌室10，被布置成覆盖入口开口11或入口开口11和出口开口12的HEPA过滤器3 无菌室10和向无菌室10供给灭菌材料如过氧化氢的灭菌材料供给部，无菌室10设置有向臭氧层过滤器3喷射臭氧等解毒材料的解毒材料供给部 。

公开(公告)号：US08741227B2

公开(公告)日：2014-06-03

申请号：US12693969

申请日：2010-01-26

申请人： Yasuhiko Yokoi ,  Jiro Ohnishi ,  Shinji Fukui ,  Akifumi Iwama

发明人： Yasuhiko Yokoi ,  Jiro Ohnishi ,  Shinji Fukui ,  Akifumi Iwama

IPC分类号： A61L2/00 ,  A61L9/00 ,  B01L1/04 ,  B01D47/00 ,  A61L2/02 ,  A61L2/20 ,  A61L2/22 ,  A61L9/015 ,  F24F13/28 ,  B01D45/00

CPC分类号： A61L9/00 ,  A61L2/0017 ,  A61L2/022 ,  A61L2/208 ,  A61L2/22 ,  A61L2/24 ,  A61L9/015 ,  A61L2202/122 ,  A61L2202/14 ,  B01D45/00 ,  C12M37/00 ,  F24F13/28

摘要： An isolator includes a workroom having a first sterilizing material supply port, a first sterilizing material discharge port, a first gas supply port and a first gas discharge port, a sterilization chamber having a second sterilizing material supply port, a second sterilizing material discharge port, a second gas supply port and a second gas discharge port, a sterilizing material supply unit, a first sterilizing material supply path connecting the sterilizing material supply unit to the first sterilizing material supply port, a second sterilizing material supply path connecting the sterilizing material supply unit to the second sterilizing material supply port, a first circulating path connecting the first sterilizing material discharge port to the sterilizing material supply unit, a second circulating path connecting the second sterilizing material discharge port to the sterilizing material supply unit, a first gas supply path, a second gas supply path, a first gas discharge path, a second gas discharge path, and a control unit.

摘要翻译： 隔离器包括具有第一灭菌材料供给口，第一灭菌材料排出口，第一气体供给口和第一排气口的工作室，具有第二灭菌材料供给口的灭菌室，第二灭菌材料排出口， 第二供气口和第二气体排出口，灭菌材料供给单元，将灭菌材料供给单元连接到第一灭菌材料供给口的第一灭菌材料供给路径，连接灭菌材料供给单元的第二灭菌材料供给路径 第二消毒材料供给口，将第一消毒材料排出口与灭菌材料供给单元连接的第一循环路径，将第二消毒材料排出口与灭菌材料供给单元连接的第二循环路径，第一供气路径， 第二气体供给路径，第一气体排出路径 离子气体排出路径和控制单元。

公开(公告)号：US08796010B2

公开(公告)日：2014-08-05

申请号：US13035538

申请日：2011-02-25

申请人： Yasuhiko Yokoi ,  Akifumi Iwama ,  Shinji Fukui ,  Atsushi Nakao ,  Hiroshi Yamamoto ,  Jirou Ohnishi ,  Hironobu Sunayama ,  Kanjun Yamamoto

发明人： Yasuhiko Yokoi ,  Akifumi Iwama ,  Shinji Fukui ,  Atsushi Nakao ,  Hiroshi Yamamoto ,  Jirou Ohnishi ,  Hironobu Sunayama ,  Kanjun Yamamoto

IPC分类号： C12M1/38 ,  C12M1/12 ,  C12M1/00

CPC分类号： C12M37/04 ,  C12M37/00 ,  C12M41/14 ,  Y10S435/809

摘要： An isolator for cultivating cells including a working chamber having a plurality of gloves arranged side by side into which operator's hands are inserted to operate cells, the working chamber being sectioned into at least an operation area for operating the cells, and an auxiliary working area for opening a packaged auxiliary instrument used to operate the cells, a gas supply unit that supplies gas into the working chamber so that the gas flows downwardly from an upper side in the working chamber, and a gas flow control unit for controlling the flow of the downwardly flowing gas so that the gas flows from the operation area to the auxiliary working area around the gloves, wherein the gas flow control unit has an exhaust hole portion that has an opened area for passing the gas therethrough and is provided at a lower portion of at least the auxiliary working area, and through which the gas in the auxiliary working area is exhausted.

摘要翻译： 一种用于培养细胞的隔离器，包括具有并排设置的多个手套的工作室，操作者的手被插入操作单元中，所述工作室被分成至少一个用于操作所述单元的操作区域，以及辅助工作区域 打开用于操作细胞的包装的辅助仪器，气体供应单元，其将气体供应到工作室中，使得气体从工作室中的上侧向下流动;气体流量控制单元，用于控制向下的流动 流动气体，使得气体从操作区域流到手套周围的辅助工作区域，其中气体流量控制单元具有排气孔部分，该排气孔部分具有用于使气体通过的开口区域，并且设置在下部 至少辅助工作区域，辅助工作区域中的气体通过其排出。

公开(公告)号：US08658107B2

公开(公告)日：2014-02-25

申请号：US12863080

申请日：2009-08-20

申请人： Yasuhiko Yokoi ,  Jiro Ohnishi ,  Akifumi Iwama ,  Masaki Harada ,  Yoshiaki Noguchi

发明人： Yasuhiko Yokoi ,  Jiro Ohnishi ,  Akifumi Iwama ,  Masaki Harada ,  Yoshiaki Noguchi

IPC分类号： A61L2/00 ,  B01D47/00 ,  B01L1/04

CPC分类号： B01L1/02 ,  A61L2/0094 ,  A61L2/025 ,  A61L2/208 ,  A61L2202/11 ,  A61L2202/122 ,  A61L2202/13 ,  A61L2202/14 ,  B01L2300/14 ,  C12M37/00 ,  G01M3/26

摘要： An isolator includes a work chamber, a sterilizing substance supply unit, a gas flow channel pressure adjustment unit, a work chamber barometer, and a controller. The controller is configured to control execution of a gas flow channel leak test for checking a gas leak in a gas flow channel based on a detection result by the work chamber barometer after making the gas flow channel pressure adjustment unit increase or decrease the pressure in the gas flow channel, and is configured to control supply of the sterilizing substance by the sterilizing substance supply unit. The controller performs heating of a heater, which accompanies the supply of the sterilizing substance, in parallel with the gas flow channel leak test.

摘要翻译： 隔离器包括工作室，灭菌物质供给单元，气体流路压力调节单元，工作室气压计和控制器。 控制器被配置为在使气体流路压力调节单元增加或减小气体流动通道压力调节单元中的压力之后，基于工作室气压计的检测结果来控制用于检查气体流路中的气体泄漏的气体流路泄漏测试的执行 气体流路，被配置为控制由灭菌物质供给部供给灭菌物质。 与气体流路泄漏试验并行地进行伴随灭菌物质的供给的加热器的加热。

公开(公告)号：US20110058986A1

公开(公告)日：2011-03-10

申请号：US12863080

申请日：2009-08-20

申请人： Yasuhiko Yokoi ,  Jiro Ohnishi ,  Akifumi Iwama ,  Masaki Harada ,  Yoshiaki Noguchi

发明人： Yasuhiko Yokoi ,  Jiro Ohnishi ,  Akifumi Iwama ,  Masaki Harada ,  Yoshiaki Noguchi

IPC分类号： G05D7/00

CPC分类号： B01L1/02 ,  A61L2/0094 ,  A61L2/025 ,  A61L2/208 ,  A61L2202/11 ,  A61L2202/122 ,  A61L2202/13 ,  A61L2202/14 ,  B01L2300/14 ,  C12M37/00 ,  G01M3/26

摘要： An isolator includes a work chamber, a sterilizing substance supply unit, a gas flow channel pressure adjustment unit, a work chamber barometer, and a controller. The controller is configured to control execution of a gas flow channel leak test for checking a gas leak in a gas flow channel based on a detection result by the work chamber barometer after making the gas flow channel pressure adjustment unit increase or decrease the pressure in the gas flow channel, and is configured to control supply of the sterilizing substance by the sterilizing substance supply unit. The controller performs heating of a heater, which accompanies the supply of the sterilizing substance, in parallel with the gas flow channel leak test.

摘要翻译： 隔离器包括工作室，灭菌物质供给单元，气体流路压力调节单元，工作室气压计和控制器。 控制器被配置为在使气体流路压力调节单元增加或减小气体流动通道压力调节单元中的压力之后，基于工作室气压计的检测结果来控制用于检查气体流路中的气体泄漏的气体流路泄漏测试的执行 气体流路，被配置为控制由灭菌物质供给部供给灭菌物质。 与气体流路泄漏试验并行地进行伴随灭菌物质的供给的加热器的加热。

公开(公告)号：US20110027131A1

公开(公告)日：2011-02-03

申请号：US12731708

申请日：2010-03-25

申请人： Yasuhiko Yokoi ,  Jiro Ohnishi ,  Shinji Fukui ,  Akifumi Iwama ,  Hiroshi Yamamoto ,  Masaki Harada ,  Tatsuya Hirose

发明人： Yasuhiko Yokoi ,  Jiro Ohnishi ,  Shinji Fukui ,  Akifumi Iwama ,  Hiroshi Yamamoto ,  Masaki Harada ,  Tatsuya Hirose

IPC分类号： A61L2/16 ,  A61L2/20

CPC分类号： A61L2/208 ,  A61L2/22 ,  A61L2/26 ,  A61L2202/11

摘要： A sterile-substance-supplying apparatus comprising: an atomization unit including a reservoir unit to reserve hydrogen peroxide, and an ultrasound vibrator to impart ultrasound vibration to the reserved hydrogen peroxide to be atomized; an evaporation unit, provided above the reservoir unit, that heats the atomized hydrogen peroxide to be evaporated; a connecting member connecting the evaporation unit and the atomization unit to each other; a hydrogen-peroxide-supply port to allow the hydrogen peroxide to be supplied to the reservoir unit; a carrier-gas-supply port, provided at the connecting member, that allows carrier gas for allowing the atomized hydrogen peroxide to flow to the evaporation unit to be supplied; and a container portion to reserve a substance for transmitting the ultrasound vibration generated by the ultrasound vibrator, and a metal vibrating plate, provided at a bottom portion of the reservoir unit, that transmits to the hydrogen peroxide the ultrasound vibration transmitted through the substance.

摘要翻译： 1.一种无菌物质供给装置，其特征在于，包括：雾化单元，其具有储存单元以保留过氧化氢;以及超声波振动器，用于对预定的要被雾化的过氧化氢赋予超声波振动; 设置在储存器单元上方的蒸发单元，其加热待蒸发的雾化过氧化氢; 将蒸发单元和雾化单元彼此连接的连接构件; 一个过氧化氢供应口，以允许过氧化氢被供应到贮存器单元; 设置在所述连接构件上的载气供给口，其允许载气使所述雾化过氧化氢流入所述蒸发单元供给; 以及容纳部，其保留用于传输由超声波振动器产生的超声波振动的物质;以及金属振动板，其设置在所述储存器单元的底部，所述金属振动板向所述过氧化氢传递通过所述物质传播的超声波振动。

公开(公告)号：US20100189607A1

公开(公告)日：2010-07-29

申请号：US12693969

申请日：2010-01-26

申请人： Yasuhiko Yokoi ,  Jiro Ohnishi ,  Shinji Fukui ,  Akifumi Iwama

发明人： Yasuhiko Yokoi ,  Jiro Ohnishi ,  Shinji Fukui ,  Akifumi Iwama

IPC分类号： A61L2/24 ,  A61L2/00 ,  A61L2/16

CPC分类号： A61L9/00 ,  A61L2/0017 ,  A61L2/022 ,  A61L2/208 ,  A61L2/22 ,  A61L2/24 ,  A61L9/015 ,  A61L2202/122 ,  A61L2202/14 ,  B01D45/00 ,  C12M37/00 ,  F24F13/28

摘要： An isolator includes a workroom having a first sterilizing material supply port, a first sterilizing material discharge port, a first gas supply port and a first gas discharge port, a sterilization chamber having a second sterilizing material supply port, a second sterilizing material discharge port, a second gas supply port and a second gas discharge port, a sterilizing material supply unit, a first sterilizing material supply path connecting the sterilizing material supply unit to the first sterilizing material supply port, a second sterilizing material supply path connecting the sterilizing material supply unit to the second sterilizing material supply port, a first circulating path connecting the first sterilizing material discharge port to the sterilizing material supply unit, a second circulating path connecting the second sterilizing material discharge port to the sterilizing material supply unit, a first gas supply path, a second gas supply path, a first gas discharge path, a second gas discharge path, and a control unit.

摘要翻译： 隔离器包括具有第一灭菌材料供给口，第一灭菌材料排出口，第一气体供给口和第一排气口的工作室，具有第二灭菌材料供给口的灭菌室，第二灭菌材料排出口， 第二供气口和第二气体排出口，灭菌材料供给单元，将灭菌材料供给单元连接到第一灭菌材料供给口的第一灭菌材料供给路径，连接灭菌材料供给单元的第二灭菌材料供给路径 第二消毒材料供给口，将第一消毒材料排出口与灭菌材料供给单元连接的第一循环路径，将第二消毒材料排出口与灭菌材料供给单元连接的第二循环路径，第一供气路径， 第二气体供给路径，第一气体排出路径 离子气体排出路径和控制单元。