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公开(公告)号：US20110027874A1
公开(公告)日：2011-02-03
申请号：US12899924
申请日：2010-10-07
IPC分类号： C12M1/00
CPC分类号： C12Q1/6834 , C12Q1/686 , C12Q2547/101 , C12Q2531/113 , C12Q2565/518
摘要： A system for nucleic acid capture and amplification comprising introducing a sample potentially containing the nucleic acid into a packed bed wherein the nucleic acid adheres to the packed bed, introducing an amplification mix into the packed bed, and thermal cycling the packed bed and the nucleic acid between denaturation and annealing temperatures for PCR amplification. One embodiment provides an apparatus for DNA capture and amplification comprising a tubing or housing having a cavity, bed media in the cavity, and a heater operatively connected to the tubing or housing.
摘要翻译： 一种用于核酸捕获和扩增的系统，包括将可能含有核酸的样品引入填充床中，其中核酸粘附到填充床，将扩增混合物引入填充床中，以及热循环填充床和核酸 在变性和退火温度之间进行PCR扩增。 一个实施例提供了一种用于DNA捕获和扩增的装置，其包括具有空腔的管或壳体，空腔中的床介质和可操作地连接到管道或壳体的加热器。
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公开(公告)号：US20190299209A1
公开(公告)日：2019-10-03
申请号：US16367125
申请日：2019-03-27
摘要： A multi-channel optical detection system includes a base unit adapted to receive a multi-chamber assay cartridge having a plurality of reaction chambers loaded with a sample and an optical detection reagent, such as for example a fluorescence detection reagent, and an optical detection unit having a multi-channel optical block having a plurality of detection channels each with an associated light source, and an optic sensor. The optical detection unit is connectable to the base unit so that interrogation ports of the detection channels are optically aligned with optically transparent windows of the reaction chambers of a loaded cartridge. In an example embodiment, connecting the optical detection unit to the base unit initializes the optical detection process which includes activating the lights sources to direct an interrogating light beam into the reaction chambers to interrogate reaction products produced therein, and controlling the optic sensors to detect the optical responses from the interrogated reaction products in the reaction chambers. If a heating element is provided in the base unit, connecting the optical detection unit to the base unit may also trigger heating of the reaction chambers prior to initializing the optical detection process.
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公开(公告)号：US20090209030A1
公开(公告)日：2009-08-20
申请号：US12415623
申请日：2009-03-31
申请人： William J. Benett , James B. Richards , Paul L. Stratton , Elizabeth K. Wheeler , Peter Krulevitch , Steve Visuri , John M. Dzenitis
发明人： William J. Benett , James B. Richards , Paul L. Stratton , Elizabeth K. Wheeler , Peter Krulevitch , Steve Visuri , John M. Dzenitis
CPC分类号： B01L7/52 , B01L3/5082 , B01L2200/12 , B01L2300/123 , B01L2300/1827 , B01L2300/1844 , Y10T29/49124
摘要： A thermalcycler is constructing with a first thermalcycler body section of a flexible circuit material or a circuit board material. The first thermalcycler body section has a first face. A first cavity portion is formed in the first face. A second thermalcycler body section is constructed of a flexible circuit material or a circuit board material. The second thermalcycler body section has a second face. A second cavity portion is formed in the second face. When the first cavity portion and the second cavity portion are positioned together they form a cavity. A thermalcycler unit is positioned in the cavity. The first thermalcycler body section and the second thermalcycler body section are connected together with the first face and the face opposed to each other and the thermalcycler unit operatively connected to the first cavity portion and the second cavity portion.
摘要翻译： 热循环器正在构造具有柔性电路材料或电路板材料的第一热循环体主体部分。 第一热循环体主体部分具有第一面。 第一空腔部分形成在第一面中。 第二个热循环器主体部分由柔性电路材料或电路板材料构成。 第二热循环器本体部分具有第二面。 第二空腔部分形成在第二面中。 当第一空腔部分和第二空腔部分位于一起时，它们形成空腔。 热循环器单元位于空腔中。 第一热循环器主体部分和第二热循环器主体部分与第一面和彼此相对的面连接在一起，并且热循环器单元可操作地连接到第一腔部分和第二腔部分。
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公开(公告)号：US11975321B2
公开(公告)日：2024-05-07
申请号：US16367125
申请日：2019-03-27
CPC分类号： B01L3/502715 , G01N21/76 , B01L2200/10
摘要： A multi-channel optical detection system includes a base unit adapted to receive a multi-chamber assay cartridge having a plurality of reaction chambers loaded with a sample and an optical detection reagent, and an optical detection unit having a multi-channel optical block having a plurality of detection channels each with an associated light source, and an optic sensor. The optical detection unit is connectable to the base unit so that interrogation ports of the detection channels are optically aligned with optically transparent windows of the reaction chambers of a loaded cartridge, so that upon initialization, light sources are activated to interrogate reaction products in the reaction chambers and detect the optical responses therefrom.
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公开(公告)号：US07504265B2
公开(公告)日：2009-03-17
申请号：US11447754
申请日：2006-06-05
IPC分类号： G01N33/543
CPC分类号： G01N15/1404 , G01N15/1484 , G01N2015/0065 , G01N2015/142 , Y10S436/80 , Y10S436/805 , Y10T436/117497
摘要： A flow cytometer includes a flow cell for detecting the sample, an oil phase in the flow cell, a water phase in the flow cell, an oil-water interface between the oil phase and the water phase, a detector for detecting the sample at the oil-water interface, and a hydrophobic unit operatively connected to the sample. The hydrophobic unit is attached to the sample. The sample and the hydrophobic unit are placed in an oil and water combination. The sample is detected at the interface between the oil phase and the water phase.
摘要翻译： 流式细胞仪包括用于检测样品的流动池，流动池中的油相，流动池中的水相，油相和水相之间的油 - 水界面，用于在该油相和水相之间检测样品的检测器 油 - 水界面和可操作地连接到样品的疏水单元。 疏水单元附着在样品上。 将样品和疏水单元置于油水组合中。 在油相和水相之间的界面处检测样品。
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公开(公告)号：US07081227B2
公开(公告)日：2006-07-25
申请号：US10862998
申请日：2004-06-07
IPC分类号： G01N21/00
CPC分类号： G01N15/1404 , G01N15/1484 , G01N2015/0065 , G01N2015/142 , Y10S436/80 , Y10S436/805 , Y10T436/117497
摘要： A flow cytometer includes a flow cell for detecting the sample, an oil phase in the flow cell, a water phase in the flow cell, an oil-water interface between the oil phase and the water phase, a detector for detecting the sample at the oil-water interface, and a hydrophobic unit operatively connected to the sample. The hydrophobic unit is attached to the sample. The sample and the hydrophobic unit are placed in an oil and water combination. The sample is detected at the interface between the oil phase and the water phase.
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