公开(公告)号：US20100166608A1

公开(公告)日：2010-07-01

申请号：US12645396

申请日：2009-12-22

申请人： Emerson Chueng Quan ,  Colin Jon Taylor ,  Michael Lee ,  Christopher G. Ceasar ,  Greg Harris ,  Gang Sun

发明人： Emerson Chueng Quan ,  Colin Jon Taylor ,  Michael Lee ,  Christopher G. Ceasar ,  Greg Harris ,  Gang Sun

IPC分类号： B01J19/00

CPC分类号： G01N33/00 ,  B01L3/5025 ,  B01L3/502707 ,  B01L2200/025 ,  B01L2200/12 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2300/0887 ,  B01L2400/0481 ,  B01L2400/0655 ,  Y10T436/11

摘要： A biological substrate, e.g., microfluidic chip. The substrate includes a rigid substrate material, which has a surface region capable of acting as a handle substrate. The substrate also has a deformable fluid layer coupled to the surface region. One or more well regions are formed in a first portion of the deformable fluid layer and are capable of holding a fluid therein. The one or more channel regions are formed in a second portion of the deformable fluid layer and are coupled to one or more of the well regions. An active region is formed in the deformable fluid layer. At least three fiducial markings are formed within the non-active region and disposed in a spatial manner associated with at least one of the well regions. A control layer is coupled to the fluid layer.

摘要翻译： 生物底物，例如微流体芯片。 衬底包括刚性衬底材料，其具有能够用作手柄衬底的表面区域。 衬底还具有耦合到表面区域的可变形流体层。 一个或多个阱区域形成在可变形流体层的第一部分中并且能够在其中保持流体。 一个或多个通道区域形成在可变形流体层的第二部分中并且耦合到一个或多个阱区域。 在可变形流体层中形成有源区。 在非有源区域内形成至少三个基准标记，并以与至少一个阱区域相关联的空间方式设置。 控制层耦合到流体层。

公开(公告)号：US08367016B2

公开(公告)日：2013-02-05

申请号：US13360591

申请日：2012-01-27

申请人： Emerson Chueng Quan ,  Colin Jon Taylor ,  Michael Lee ,  Christopher G. Ceasar ,  Greg Harris ,  Gang Sun

发明人： Emerson Chueng Quan ,  Colin Jon Taylor ,  Michael Lee ,  Christopher G. Ceasar ,  Greg Harris ,  Gang Sun

IPC分类号： G01N33/00

CPC分类号： G01N33/00 ,  B01L3/5025 ,  B01L3/502707 ,  B01L2200/025 ,  B01L2200/12 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2300/0887 ,  B01L2400/0481 ,  B01L2400/0655 ,  Y10T436/11

摘要： A method for producing an image of an object within a chamber of a microfluidic device includes providing the microfluidic device having x, y, and z dimensions and a chamber depth center point located along the z dimension. The chamber depth center point is located a known z dimension distance from a fiducial marking embedded within the microfluidic device. The method also includes placing the microfluidic device within an imaging system that includes an optical device capable of detecting the fiducial marking. The optical device defines an optical path axially aligned with the z dimension and has a focal plane perpendicular to the optical path. When the focal plane is moved along the optical path, the fiducial marking is maximally detected when the focal plane is at the z depth in comparison to when the focal plane is not substantially in-plane with the z depth.

摘要翻译： 用于在微流体装置的腔室内产生物体的图像的方法包括提供具有x，y和z尺寸的微流体装置以及沿着z维度定位的腔室中心点。 腔室深度中心点位于与嵌入在微流体装置内的基准标记的已知z维距离。 该方法还包括将微流体装置放置在包括能够检测基准标记的光学装置的成像系统内。 光学装置限定与z尺寸轴向对准的光路，并且具有垂直于光路的焦平面。 当焦平面沿着光路移动时，当焦平面与z深度基本上不在平面内时，当焦平面处于z深度时，基准标记被最大程度地检测。

公开(公告)号：US20120242825A1

公开(公告)日：2012-09-27

申请号：US13360591

申请日：2012-01-27

申请人： Emerson Chueng Quan ,  Colin Jon Taylor ,  Michael Lee ,  Christopher G. Cesar ,  Greg Harris ,  Gang Sun

发明人： Emerson Chueng Quan ,  Colin Jon Taylor ,  Michael Lee ,  Christopher G. Cesar ,  Greg Harris ,  Gang Sun

IPC分类号： H04N7/18

CPC分类号： G01N33/00 ,  B01L3/5025 ,  B01L3/502707 ,  B01L2200/025 ,  B01L2200/12 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2300/0887 ,  B01L2400/0481 ,  B01L2400/0655 ,  Y10T436/11

摘要： A method for producing an image of an object within a chamber of a microfluidic device includes providing the microfluidic device having x, y, and z dimensions and a chamber depth center point located along the z dimension. The chamber depth center point is located a known z dimension distance from a fiducial marking embedded within the microfluidic device. The method also includes placing the microfluidic device within an imaging system that includes an optical device capable of detecting the fiducial marking. The optical device defines an optical path axially aligned with the z dimension and has a focal plane perpendicular to the optical path. When the focal plane is moved along the optical path, the fiducial marking is maximally detected when the focal plane is at the z depth in comparison to when the focal plane is not substantially in-plane with the z depth.

摘要翻译： 用于在微流体装置的腔室内产生物体的图像的方法包括提供具有x，y和z尺寸的微流体装置以及沿着z维度定位的腔室中心点。 腔室深度中心点位于与嵌入在微流体装置内的基准标记的已知z维距离。 该方法还包括将微流体装置放置在包括能够检测基准标记的光学装置的成像系统内。 光学装置限定与z尺寸轴向对准的光路，并且具有垂直于光路的焦平面。 当焦平面沿着光路移动时，当焦平面与z深度基本上不在平面内时，当焦平面处于z深度时，基准标记被最大程度地检测。

公开(公告)号：US07695683B2

公开(公告)日：2010-04-13

申请号：US10851777

申请日：2004-05-20

申请人： Emerson Chueng Quan ,  Colin Jon Taylor ,  Michael Lee ,  Christopher G. Cesar ,  Greg Harris ,  Gang Sun

发明人： Emerson Chueng Quan ,  Colin Jon Taylor ,  Michael Lee ,  Christopher G. Cesar ,  Greg Harris ,  Gang Sun

IPC分类号： G01N33/00

CPC分类号： G01N33/00 ,  B01L3/5025 ,  B01L3/502707 ,  B01L2200/025 ,  B01L2200/12 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2300/0887 ,  B01L2400/0481 ,  B01L2400/0655 ,  Y10T436/11

摘要： A biological substrate, e.g., microfluidic chip. The substrate includes a rigid substrate material, which has a surface region capable of acting as a handle substrate. The substrate also has a deformable fluid layer coupled to the surface region. One or more well regions are formed in a first portion of the deformable fluid layer and are capable of holding a fluid therein. The one or more channel regions are formed in a second portion of the deformable fluid layer and are coupled to one or more of the well regions. An active region is formed in the deformable fluid layer. At least three fiducial markings are formed within the non-active region and disposed in a spatial manner associated with at least one of the well regions. A control layer is coupled to the fluid layer.

摘要翻译： 生物底物，例如微流体芯片。 衬底包括刚性衬底材料，其具有能够用作手柄衬底的表面区域。 衬底还具有耦合到表面区域的可变形流体层。 一个或多个阱区域形成在可变形流体层的第一部分中并且能够在其中保持流体。 一个或多个通道区域形成在可变形流体层的第二部分中并且耦合到一个或多个阱区域。 在可变形流体层中形成有源区。 在非有源区域内形成至少三个基准标记，并以与至少一个阱区域相关联的空间方式设置。 控制层耦合到流体层。

公开(公告)号：US20090299545A1

公开(公告)日：2009-12-03

申请号：US10851777

申请日：2004-05-20

申请人： Emerson Chueng Quan ,  Colin Jon Taylor ,  Michael Lee ,  Christopher G. Cesar ,  Greg Harris ,  Gang Sun

发明人： Emerson Chueng Quan ,  Colin Jon Taylor ,  Michael Lee ,  Christopher G. Cesar ,  Greg Harris ,  Gang Sun

IPC分类号： G05D3/12 ,  G06K9/00

CPC分类号： G01N33/00 ,  B01L3/5025 ,  B01L3/502707 ,  B01L2200/025 ,  B01L2200/12 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2300/0887 ,  B01L2400/0481 ,  B01L2400/0655 ,  Y10T436/11

摘要： A biological substrate, e.g., microfluidic chip. The substrate includes a rigid substrate material, which has a surface region capable of acting as a handle substrate. The substrate also has a deformable fluid layer coupled to the surface region. One or more well regions are formed in a first portion of the deformable fluid layer and are capable of holding a fluid therein. The one or more channel regions are formed in a second portion of the deformable fluid layer and are coupled to one or more of the well regions. An active region is formed in the deformable fluid layer. At least three fiducial markings are formed within the non-active region and disposed in a spatial manner associated with at least one of the well regions. A control layer is coupled to the fluid layer.

摘要翻译： 生物底物，例如微流体芯片。 衬底包括刚性衬底材料，其具有能够用作手柄衬底的表面区域。 衬底还具有耦合到表面区域的可变形流体层。 一个或多个阱区域形成在可变形流体层的第一部分中并且能够在其中保持流体。 一个或多个通道区域形成在可变形流体层的第二部分中并且耦合到一个或多个阱区域。 在可变形流体层中形成有源区。 在非有源区域内形成至少三个基准标记，并以与至少一个阱区域相关联的空间方式设置。 控制层耦合到流体层。

公开(公告)号：US08105550B2

公开(公告)日：2012-01-31

申请号：US12645396

申请日：2009-12-22

申请人： Emerson Chueng Quan ,  Colin Jon Taylor ,  Michael Lee ,  Christopher G. Cesar ,  Greg Harris ,  Gang Sun

发明人： Emerson Chueng Quan ,  Colin Jon Taylor ,  Michael Lee ,  Christopher G. Cesar ,  Greg Harris ,  Gang Sun

IPC分类号： G01N33/00

CPC分类号： G01N33/00 ,  B01L3/5025 ,  B01L3/502707 ,  B01L2200/025 ,  B01L2200/12 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2300/0887 ,  B01L2400/0481 ,  B01L2400/0655 ,  Y10T436/11

摘要： A biological substrate, e.g., microfluidic chip. The substrate includes a rigid substrate material, which has a surface region capable of acting as a handle substrate. The substrate also has a deformable fluid layer coupled to the surface region. One or more well regions are formed in a first portion of the deformable fluid layer and are capable of holding a fluid therein. The one or more channel regions are formed in a second portion of the deformable fluid layer and are coupled to one or more of the well regions. An active region is formed in the deformable fluid layer. At least three fiducial markings are formed within the non-active region and disposed in a spatial manner associated with at least one of the well regions. A control layer is coupled to the fluid layer.

摘要翻译： 生物底物，例如微流体芯片。 衬底包括刚性衬底材料，其具有能够用作手柄衬底的表面区域。 衬底还具有耦合到表面区域的可变形流体层。 一个或多个阱区域形成在可变形流体层的第一部分中并且能够在其中保持流体。 一个或多个通道区域形成在可变形流体层的第二部分中并且耦合到一个或多个阱区域。 在可变形流体层中形成有源区。 在非有源区域内形成至少三个基准标记，并以与至少一个阱区域相关联的空间方式设置。 控制层耦合到流体层。

公开(公告)号：US20100119154A1

公开(公告)日：2010-05-13

申请号：US12552208

申请日：2009-09-01

申请人： Colin Jon Taylor ,  Gang Sun ,  Simant Dube

发明人： Colin Jon Taylor ,  Gang Sun ,  Simant Dube

IPC分类号： G06K9/46 ,  G06K9/62

CPC分类号： G06K9/36 ,  B01L3/5027 ,  G06K9/00 ,  G06K9/00127 ,  G06K9/00134 ,  G06K9/6232 ,  G06T7/0008 ,  G06T7/0012 ,  G06T7/254 ,  G06T7/33 ,  G06T2207/30004

摘要： A method for processing an image of a microfluidic device. The method includes receiving a first image of a microfluidic device. The first image corresponds to a first state. Additionally, the method includes receiving a second image of the microfluidic device. The second image corresponds to a second state. Moreover, the method includes transforming the first image and the second image into a third coordinate space. Also, the method includes obtaining a third image based on at least information associated with the transformed first image and the transformed second image, and processing the third image to obtain information associated with the first state and the second state.

摘要翻译： 一种用于处理微流体装置的图像的方法。 该方法包括接收微流体装置的第一图像。 第一图像对应于第一状态。 另外，该方法包括接收微流体装置的第二图像。 第二图像对应于第二状态。 此外，该方法包括将第一图像和第二图像变换为第三坐标空间。 此外，该方法包括至少基于与变换的第一图像和变换的第二图像相关联的信息获得第三图像，并且处理第三图像以获得与第一状态和第二状态相关联的信息。

公开(公告)号：US07792345B2

公开(公告)日：2010-09-07

申请号：US12552208

申请日：2009-09-01

申请人： Colin Jon Taylor ,  Gang Sun ,  Simant Dube

发明人： Colin Jon Taylor ,  Gang Sun ,  Simant Dube

IPC分类号： G06K9/00

CPC分类号： G06K9/36 ,  B01L3/5027 ,  G06K9/00 ,  G06K9/00127 ,  G06K9/00134 ,  G06K9/6232 ,  G06T7/0008 ,  G06T7/0012 ,  G06T7/254 ,  G06T7/33 ,  G06T2207/30004

摘要： A method for processing an image of a microfluidic device. The method includes receiving a first image of a microfluidic device. The first image corresponds to a first state. Additionally, the method includes receiving a second image of the microfluidic device. The second image corresponds to a second state. Moreover, the method includes transforming the first image and the second image into a third coordinate space. Also, the method includes obtaining a third image based on at least information associated with the transformed first image and the transformed second image, and processing the third image to obtain information associated with the first state and the second state.

摘要翻译： 一种用于处理微流体装置的图像的方法。 该方法包括接收微流体装置的第一图像。 第一图像对应于第一状态。 另外，该方法包括接收微流体装置的第二图像。 第二图像对应于第二状态。 此外，该方法包括将第一图像和第二图像变换为第三坐标空间。 此外，该方法包括至少基于与变换的第一图像和变换的第二图像相关联的信息获得第三图像，并且处理第三图像以获得与第一状态和第二状态相关联的信息。

公开(公告)号：US07583853B2

公开(公告)日：2009-09-01

申请号：US10902494

申请日：2004-07-28

申请人： Colin Jon Taylor ,  Gang Sun ,  Simant Dube

发明人： Colin Jon Taylor ,  Gang Sun ,  Simant Dube

IPC分类号： G06K9/36

CPC分类号： G06K9/36 ,  B01L3/5027 ,  G06K9/00 ,  G06K9/00127 ,  G06K9/00134 ,  G06K9/6232 ,  G06T7/0008 ,  G06T7/0012 ,  G06T7/254 ,  G06T7/33 ,  G06T2207/30004

摘要： A method for processing an image of a microfluidic device. The method includes receiving a first image of a microfluidic device. The first image corresponds to a first state. Additionally, the method includes receiving a second image of the microfluidic device. The second image corresponds to a second state. Moreover, the method includes transforming the first image and the second image into a third coordinate space. Also, the method includes obtaining a third image based on at least information associated with the transformed first image and the transformed second image, and processing the third image to obtain information associated with the first state and the second state.

摘要翻译： 一种用于处理微流体装置的图像的方法。 该方法包括接收微流体装置的第一图像。 第一图像对应于第一状态。 另外，该方法包括接收微流体装置的第二图像。 第二图像对应于第二状态。 此外，该方法包括将第一图像和第二图像变换为第三坐标空间。 此外，该方法包括至少基于与变换的第一图像和变换的第二图像相关联的信息获得第三图像，并且处理第三图像以获得与第一状态和第二状态相关联的信息。