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    Highly alkaline proteases
    4.
    发明授权
    Highly alkaline proteases 失效
    高碱性蛋白酶

    公开(公告)号：US5352603A

    公开(公告)日：1994-10-04

    申请号：US661378

    申请日：1991-02-28

    申请人： Roman Vetter ,  Detlef Wilke ,  Antoine Amory ,  Andre Clippe ,  Dietmar Schomburg ,  Wolfgang Aehle

    发明人： Roman Vetter ,  Detlef Wilke ,  Antoine Amory ,  Andre Clippe ,  Dietmar Schomburg ,  Wolfgang Aehle

    IPC分类号： C12N1/21 ,  C12N9/54 ,  C12N15/57 ,  C11D3/386 ,  C11D7/42 ,  C12N9/52

    CPC分类号： C12N9/54

    摘要： Novel, optimized highly alkaline proteases which are suitable for use in detergent formulations are prepared by employing microorganisms transformed by mutated DNA sequences. The mutated sequences are obtained starting from DNA sequences which code for highly alkaline protease usually produced by Bacillus species by altering these DNA sequences in defined positions by directed mutagenesis (point mutation) in such a way that the codon in which the point mutation is located now codes for an amino acid which is more strongly basic than the original amino acid. The result is highly alkaline proteases in which original amino acids have been replaced by more strongly basic amino acids, preferably by the amino acids lysine or arginine. Synthetic oligonucleotides, DNA sequences, vectors and transformed microorganisms which are used for generating and obtaining the optimized highly alkaline protease are also described.

    摘要翻译： 通过使用通过突变的DNA序列转化的微生物来制备适用于洗涤剂制剂的新型优化的高碱性蛋白酶。 突变序列从编码通过芽孢杆菌属物种生产的高度碱性蛋白酶的DNA序列开始获得，通过定点诱变（点突变）以定点诱变（点突变）改变这些DNA序列，使得点突变位于的密码子现在 编码比原始氨基酸更强碱性的氨基酸。 结果是高碱性蛋白酶，其中原始氨基酸已被更强碱性氨基酸替代，优选由氨基酸赖氨酸或精氨酸代替。 还描述了用于产生和获得优化的高碱性蛋白酶的合成寡核苷酸，DNA序列，载体和转化微生物。

    Oxidizing enzymes
    5.
    发明授权
    Oxidizing enzymes 有权
    氧化酶

    公开(公告)号：US07144717B1

    公开(公告)日：2006-12-05

    申请号：US09273957

    申请日：1999-03-22

    申请人： Huaming Wang ,  Cynthia C. Wang ,  Antoine Amory ,  Patrick Dhaese ,  Annick Lambrechts-Rongvaux

    发明人： Huaming Wang ,  Cynthia C. Wang ,  Antoine Amory ,  Patrick Dhaese ,  Annick Lambrechts-Rongvaux

    IPC分类号： C12N9/02 ,  C12N9/04 ,  C12N1/14 ,  C07K1/00

    CPC分类号： C11D3/38654 ,  C12N9/0057

    摘要： Disclosed herein are phenol oxidizing enzymes obtainable from species of Stachybotrys which are useful in modifying the color associated with dyes and colored compounds, as well as in anti-dye transfer applications. Also disclosed herein are biologically-pure cultures of strains of the genus Stachybotrys, designated herein Stachybotrys parvispora MUCL 38996 and Stachybotrys charatarum MUCL 38898, which are capable of naturally-producing the novel phenol oxidizing enzymes. Disclosed herein is the amino acid and nucleic acid sequence for Stachybotrys phenol oxidizing enzymes as well as expression vectors and host cells comprising the nucleic acid. Disclosed herein are methods for producing the phenol oxidizing enzyme as well as methods for constructing expression host. Disclosed herein are enzyme compositions comprising phenol oxidizing enzymes obtainable from species of Stachybotrys.

    摘要翻译： 本文公开了可从Stachybotrys的物种获得的酚氧化酶，其可用于改变与染料和着色化合物相关的颜色以及抗染料转移应用。 本文还公开了Stachybotrys属（Stachybotrys parvispora MUCL 38996和Stachybotrys charatarum MUCL 38898）的菌株的生物纯培养物，其能够天然产生新的酚氧化酶。 本文公开了Stachybotrys酚氧化酶的氨基酸和核酸序列以及包含该核酸的表达载体和宿主细胞。 本文公开了生产酚氧化酶的方法以及构建表达载体的方法。 本文公开了包含可从Stachybotrys的物种获得的酚氧化酶的酶组合物。

    Process for the production of novel pullulanase
    6.
    发明授权
    Process for the production of novel pullulanase 失效
    生产新型支链淀粉酶的方法

    公开(公告)号：US5721128A

    公开(公告)日：1998-02-24

    申请号：US477630

    申请日：1995-06-07

    申请人： Philippe Deweer ,  Antoine Amory

    发明人： Philippe Deweer ,  Antoine Amory

    IPC分类号： C12N1/21 ,  C12N9/44 ,  C12N15/09 ,  C12N15/56 ,  C12P19/16 ,  C12R1/07

    CPC分类号： C12N9/2457 ,  C12N9/2451 ,  C12P19/16 ,  C12R1/07 ,  C12Y302/01041

    摘要： The invention relates to a heat-stable pullulanase having the property of hydrolysing glucosidic bonds of the .alpha.-1,6 type in amylopectin and having an enzymatic activity in an acid medium and at a temperature of about 60.degree. C. The invention also relates to strains of micro-organisms which produce this pullulanase and processes for the preparation of this pullulanase. The invention also relates to the uses thereof and compositions comprising the product. The invention also relates to a DNA molecule. The invention relates to an expression vector containing this DNA molecule and to a chromosomal integration vector containing this DNA molecule.

    摘要翻译： 本发明涉及一种热稳定的支链淀粉酶，其具有在支链淀粉中水解α-1,6型糖苷键的性质，并且在酸性培养基中和在约60℃的温度下具有酶活性。本发明还涉及 产生这种支链淀粉酶的微生物菌株和制备该支链淀粉酶的方法。 本发明还涉及其用途和包含该产品的组合物。 本发明还涉及DNA分子。 本发明涉及包含该DNA分子的表达载体和涉及含有该DNA分子的染色体整合载体。

    Pullulanase, microorganisms which produce it, processes for the preparation of this pullulanese and the uses thereof
    9.
    发明授权
    Pullulanase, microorganisms which produce it, processes for the preparation of this pullulanese and the uses thereof 失效
    支链淀粉酶，产生它的微生物，用于制备该支链淀粉及其用途

    公开(公告)号：US6074854A

    公开(公告)日：2000-06-13

    申请号：US996733

    申请日：1997-12-23

    申请人： Philippe Deweer ,  Antoine Amory

    发明人： Philippe Deweer ,  Antoine Amory

    IPC分类号： C12N1/21 ,  C12N9/44 ,  C12N15/09 ,  C12N15/56 ,  C12P19/16 ,  C12R1/07 ,  C12P19/14 ,  C12P19/12

    CPC分类号： C12N9/2457 ,  C12N9/2451 ,  C12P19/16 ,  C12R1/07 ,  C12Y302/01041

    摘要： The invention relates to a heat-stable pullulanase having the property of hydrolysing glucosidic bonds of the .alpha.-1,6 type in amylopectin and having an enzymatic activity in an acid medium and at a temperature of about 60.degree. C.The invention also relates to strains of microorganisms which produce this pullulanase and processes for the preparation of this pullulanase.The invention also relates to the uses thereof and compositions comprising the product.The invention also relates to a DNA molecule. The invention relates to an expression vector containing this DNA molecule and to a chromosomal integration vector containing this DNA molecule.

    摘要翻译： 本发明涉及一种热稳定的支链淀粉酶，其具有在支链淀粉中水解α-1,6型糖苷键的性质，并且在酸性培养基中和在约60℃的温度下具有酶活性。本发明还涉及 产生这种支链淀粉酶的微生物菌株和制备该支链淀粉酶的方法。 本发明还涉及其用途和包含该产品的组合物。 本发明还涉及DNA分子。 本发明涉及包含该DNA分子的表达载体和涉及含有该DNA分子的染色体整合载体。