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公开(公告)号：US07112684B2
公开(公告)日：2006-09-26
申请号：US11022039
申请日：2004-12-22
IPC分类号： C07F9/24 , C12Q1/68 , C07D311/78
CPC分类号： C09B57/14 , C09B11/08 , C09B19/00 , C09B57/02 , G01N33/533
摘要： Dye reagents useful in labeling biological materials are provided along with methods for their use.
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公开(公告)号：US06972339B2
公开(公告)日：2005-12-06
申请号：US10026374
申请日：2001-12-21
CPC分类号： C09B57/14 , C09B11/08 , C09B19/00 , C09B57/02 , G01N33/533
摘要： Dye reagents useful in labeling biological materials are provided along with methods for their use.
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公开(公告)号：US08569516B2
公开(公告)日：2013-10-29
申请号：US11357750
申请日：2006-02-17
IPC分类号： C07D311/82
CPC分类号： C07D311/78 , C07D493/10 , C07D493/20 , C07D497/10 , C07D497/20 , C07H21/04
摘要： Compounds useful in labeling biological materials are provided along with methods for their use.
摘要翻译： 提供了用于标记生物材料的化合物及其使用方法。
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公开(公告)号：US07601851B2
公开(公告)日：2009-10-13
申请号：US11127040
申请日：2005-05-10
IPC分类号： C07D311/82 , C07D311/86 , C12Q1/68
CPC分类号： C09B57/14 , C09B11/08 , C09B19/00 , C09B57/02 , G01N33/533
摘要： Dye reagents useful in labeling biological materials are provided along with methods for their use.
摘要翻译： 提供用于标记生物材料的染料试剂及其使用方法。
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公开(公告)号：US07851606B2
公开(公告)日：2010-12-14
申请号：US12541884
申请日：2009-08-14
IPC分类号： C07H21/04 , C07C309/00
摘要： The present invention provides a negatively charged minor groove binding compound, oligonucleotide conjugates comprising the same, and methods for using the same. The negatively charged minor groove binding compounds of the present invention comprises an acidic moiety that is capable of being ionized under physiological conditions. In particular, the negatively charged minor groove binding compound of the present invention comprises a binding moiety that binds preferentially into a minor groove of a double, triple or higher stranded DNA, RNA, PNA or hybrids thereof. The binding moiety comprises at least one aryl moiety and an acidic moiety which is covalently attached to a phenyl portion of the aryl moiety or to a heteroatom of a heteroaryl portion of the aryl moiety.
摘要翻译： 本发明提供带负电荷的小沟结合化合物，包含其的寡核苷酸缀合物及其使用方法。 本发明的带负电荷的小沟结合化合物包含能够在生理条件下电离的酸性部分。 特别地，本发明的带负电荷的小沟结合化合物包含优先结合双重，三重或更高链DNA，RNA，PNA或其杂交体的小沟的结合部分。 结合部分包含至少一个芳基部分和酸性部分，其共价连接到芳基部分的苯基部分或芳基部分的杂芳基部分的杂原子。
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公开(公告)号：US07582739B2
公开(公告)日：2009-09-01
申请号：US10507267
申请日：2003-03-11
IPC分类号： C07H21/04
摘要： The present invention provides a negatively charged minor groove binding compound, oligonucleotide conjugates comprising the same, and methods for using the same. The negatively charged minor groove binding compounds of the present invention comprises an acidic moiety that is capable of being ionized under physiological conditions. In particular, the negatively charged minor groove binding compound of the present invention comprises a binding moiety that binds preferentially into a minor groove of a double, triple or higher stranded DNA, RNA, PNA or hybrids thereof. The binding moiety comprises at least one aryl moiety and an acidic moiety which is covalently attached to a phenyl portion of the aryl moiety or to a heteroatom of a heteroaryl portion of the aryl moiety.
摘要翻译： 本发明提供带负电荷的小沟结合化合物，包含其的寡核苷酸缀合物及其使用方法。 本发明的带负电荷的小沟结合化合物包含能够在生理条件下电离的酸性部分。 特别地，本发明的带负电荷的小沟结合化合物包含优先结合双重，三重或更高链DNA，RNA，PNA或其杂交体的小沟的结合部分。 结合部分包含至少一个芳基部分和酸性部分，其共价连接到芳基部分的苯基部分或芳基部分的杂芳基部分的杂原子。
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公开(公告)号：US06790945B2
公开(公告)日：2004-09-14
申请号：US09876830
申请日：2001-06-06
IPC分类号： C07H2100
CPC分类号： C07D519/00 , C07B2200/11 , C07C245/08 , C07F9/2408 , C07F9/572 , C07F9/65522 , C07F9/6561 , C07H21/00 , C09B29/0813 , C12Q1/6818 , C40B40/00 , C12Q2565/1025 , C12Q2521/319
摘要： Oligonucleotide probes containing two labels are provided and are useful in hybridization assays. The probes can also contain a minor groove binding group.
摘要翻译： 提供了含有两个标记的寡核苷酸探针，可用于杂交测定。 探针也可以包含一个小沟结合组。
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8.发明授权Diaziridinyl-aryl and bis-[di(chloroethyl)amino]-aryl oligonucleotide conjugates and reagents for making the same 失效二氮丙啶基 - 芳基和双 - [二（氯乙基）氨基] - 芳基寡核苷酸共轭物及其制备方法
公开(公告)号：US6072046A
公开(公告)日：2000-06-06
申请号：US136779
申请日：1998-08-26
CPC分类号： C07H21/00
摘要： Diaziridinyl-aryl and bis-[di(chloroethyl)amino]-aryl oligonucleotide conjugates have a sequence that is complementary in the triplex forming sense to a target sequence in duplex nucleic acid. The diaziridinyl-aryl and bis-[di(chloroethyl)amino]-aryl oligonucleotide conjugates effectively cross-link with both strands of the targeted duplex nucleic acid.
摘要翻译： 二氮丙啶基 - 芳基和双 - [二（氯乙基）氨基] - 芳基寡核苷酸缀合物具有在双链体核酸中与三序列形成意义互补的序列至靶序列。 二氮丙啶基 - 芳基和双 - [二（氯乙基）氨基] - 芳基寡核苷酸缀合物有效地与靶双链体核酸的两条链交联。
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公开(公告)号：US5646126A
公开(公告)日：1997-07-08
申请号：US202927
申请日：1994-02-28
申请人： Yung-chi Cheng , Eugeny A. Lukhtanov , Rich B. Meyer, Jr. , Balakrishna S. Pai , Michael W. Reed , James H. Zhou
发明人： Yung-chi Cheng , Eugeny A. Lukhtanov , Rich B. Meyer, Jr. , Balakrishna S. Pai , Michael W. Reed , James H. Zhou
摘要： Oligonucleotides having approximately 8 to 18 nucleotide units and a 3'-tail which includes asteroid structure attached to the 3'-end through the A ring of the steroid skeleton and which form substantially stable duplexes at physiological temperature, have selective cytotoxic activity against certain tumor cell lines.
摘要翻译： 具有大约8至18个核苷酸单元的寡核苷酸和包含通过类固醇骨架的A环连接到3'末端并且在生理温度下形成基本上稳定的双链体的小行星结构的3'-尾部具有针对某些肿瘤的选择性细胞毒活性 细胞系。
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10.发明授权Functionalized 3-alkynyl pyrazolopyrimidine analogues as universal bases and methods of use 有权官能化3-炔基吡唑并嘧啶类似物作为通用碱基和使用方法公开(公告)号：US09085800B2
公开(公告)日：2015-07-21
申请号：US13429273
申请日：2012-03-23
申请人： Alexei Vorobiev , Eugeny A. Lukhtanov , Noah Scarr
发明人： Alexei Vorobiev , Eugeny A. Lukhtanov , Noah Scarr
CPC分类号： C12Q1/6853 , C12Q1/6846 , C12Q2525/117
摘要： 3-alkynyl inosine analogs and their uses as universal bases are provided. The inosine analogues can be incorporated into nucleic acid primers and probes. They do not significantly destabilize nucleic acid duplexes. As a result, the novel nucleic acid primers and probes incorporating the inosine analogues can be used in a variety of methods. The analogs function unexpectedly well as universal bases. Not only do they stabilize duplexes substantially more than hypoxanthine opposite A, C, T, and G but they are also recognized in primers by polymerases, allowing efficient amplification.
摘要翻译： 提供3-炔基肌苷类似物及其作为通用碱基的用途。 肌苷类似物可以并入核酸引物和探针中。 它们不会使核酸双链体显着不稳定。 因此，结合肌苷类似物的新型核酸引物和探针可以以多种方法使用。 类似物作为普遍基础出人意料地起作用。 它们不仅能够稳定双链体，而且还要与A，C，T和G相反的次黄嘌呤稳定，但也可以通过聚合酶在引物中识别，从而进行有效的扩增。
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