利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

33 条记录 (耗时 0.044 秒)

    Negatively charged minor groove binders
    6.
    发明授权
    Negatively charged minor groove binders 有权
    带负电荷的小槽粘合剂

    公开(公告)号：US07582739B2

    公开(公告)日：2009-09-01

    申请号：US10507267

    申请日：2003-03-11

    申请人： Eugeny A. Lukhtanov ,  Nicolaas M. J. Vermeulen ,  Alexander A. Gall

    发明人： Eugeny A. Lukhtanov ,  Nicolaas M. J. Vermeulen ,  Alexander A. Gall

    IPC分类号： C07H21/04

    CPC分类号： C07H21/04 ,  C07H21/00

    摘要： The present invention provides a negatively charged minor groove binding compound, oligonucleotide conjugates comprising the same, and methods for using the same. The negatively charged minor groove binding compounds of the present invention comprises an acidic moiety that is capable of being ionized under physiological conditions. In particular, the negatively charged minor groove binding compound of the present invention comprises a binding moiety that binds preferentially into a minor groove of a double, triple or higher stranded DNA, RNA, PNA or hybrids thereof. The binding moiety comprises at least one aryl moiety and an acidic moiety which is covalently attached to a phenyl portion of the aryl moiety or to a heteroatom of a heteroaryl portion of the aryl moiety.

    摘要翻译： 本发明提供带负电荷的小沟结合化合物，包含其的寡核苷酸缀合物及其使用方法。 本发明的带负电荷的小沟结合化合物包含能够在生理条件下电离的酸性部分。 特别地，本发明的带负电荷的小沟结合化合物包含优先结合双重，三重或更高链DNA，RNA，PNA或其杂交体的小沟的结合部分。 结合部分包含至少一个芳基部分和酸性部分，其共价连接到芳基部分的苯基部分或芳基部分的杂芳基部分的杂原子。

    Combinations and methods for reducing antimicrobial resistance
    9.
    发明授权
    Combinations and methods for reducing antimicrobial resistance 失效
    减少抗菌素耐药性的组合和方法

    公开(公告)号：US5872104A

    公开(公告)日：1999-02-16

    申请号：US364246

    申请日：1994-12-27

    申请人： Nicolaas M. J. Vermeulen ,  Dennis E. Schwartz

    发明人： Nicolaas M. J. Vermeulen ,  Dennis E. Schwartz

    IPC分类号： A61K45/06 ,  A61K31/70

    CPC分类号： A61K45/06 ,  Y10S977/80 ,  Y10S977/801

    摘要： Disclosed are novel methods, combinations of agents and kits for use in killing, or inhibiting the growth of, microorganisms. Enhanced antimicrobial action is provided by using a methylation inhibitor, as exemplified by using an agent that inhibits methylation or maturation of bacterial RNA in combination with, e.g., a macrolide lincosamide streptogramin B (MLS) antibiotic. The methods and compositions described may be employed to reduce the resistance of susceptible microorganisms to antimicrobial agents and thus to treat animals or patients with infections.

    摘要翻译： 公开了用于杀死或抑制微生物生长的新方法，试剂和试剂盒的组合。 通过使用甲基化抑制剂提供增强的抗微生物作用，例如通过使用抑制细菌RNA的甲基化或成熟的药剂与例如大环内酯氯胺素链霉素B（MLS）抗生素的组合来实施。 可以使用所描述的方法和组合来降低敏感微生物对抗微生物剂的抵抗力，从而治疗感染的动物或患者。

    Non toxic compositions and methods useful for the extraction of nucleic acids
    10.
    发明授权
    Non toxic compositions and methods useful for the extraction of nucleic acids 失效
    用于提取核酸的无毒组合物和方法

    公开(公告)号：US5393672A

    公开(公告)日：1995-02-28

    申请号：US156519

    申请日：1993-11-23

    申请人： Jeffrey V. Ness ,  B. Melina Cimler ,  Rich B. Meyer, Jr. ,  Nicolaas M. J. Vermeulen

    发明人： Jeffrey V. Ness ,  B. Melina Cimler ,  Rich B. Meyer, Jr. ,  Nicolaas M. J. Vermeulen

    IPC分类号： C12N15/10 ,  C12Q1/68 ,  G01N33/50

    CPC分类号： C12Q1/6806 ,  C12N15/1003 ,  Y10T436/143333 ,  Y10T436/25125 ,  Y10T436/25375

    摘要： This invention relates to safe and effective methods for the extraction of nucleic acids. In particular, methods are described for isolating nucleic acid from a sample containing a biological mixture of nucleic acids and other biological compounds wherein the sample is combined with an extraction solution containing at least one organic compound such as benzyl alcohol or a benzyl alcohol derivative to form an aqueous and non-aqueous phase. The nucleic acid is isolated from the aqueous phase. Preferably, the resulting combined solution also contains bentonite, as defined below. Typically, the sample will first be combined with a lysing agent before extraction. The lysing agents preferred are chaotropic salts such as guanidinium hydrochloride and guanidinium isothiocyanate.

    摘要翻译： 本发明涉及安全有效的提取核酸的方法。 特别地，描述了从含有核酸和其他生物化合物的生物混合物的样品中分离核酸的方法，其中样品与含有至少一种有机化合物如苄醇或苄醇衍生物的提取溶液组合形成 水相和非水相。 从水相中分离核酸。 优选地，所得到的组合溶液还含有如下定义的膨润土。 通常，样品首先在萃取前与裂解剂组合。 优选的裂解剂是离液盐，例如盐酸胍和异硫氰酸胍。