公开(公告)号：US20170268037A1

公开(公告)日：2017-09-21

申请号：US15310083

申请日：2015-05-15

Applicant: FLUXION BIOSCIENCES, INC.

Inventor： CRISTIAN IONESCU-ZANETTI ,  MICHAEL SCHWARTZ ,  CHRISTINE TING FU ,  RONG ANDREA FAN

IPC: C12Q1/68 ,  B03C1/28 ,  B03C1/01 ,  B03C1/033 ,  B01L3/00 ,  B03C1/30

CPC classification number: C12Q1/6806 ,  B01L3/502761 ,  B01L2200/0652 ,  B01L2400/043 ,  B03C1/01 ,  B03C1/0332 ,  B03C1/288 ,  B03C1/30 ,  B03C2201/18 ,  B03C2201/26 ,  C12M47/02 ,  C12N11/14 ,  G01N33/54326 ,  C12Q2563/143 ,  C12Q2563/149

Abstract: A method is provided including coupling magnetic beads to a population of cells in a fluid sample to form magnetically-labeled cells, magnetically separating the magnetically-labeled cells from non-magnetically-labeled cells in the fluid sample, and separating target cells from non-target cells of the magnetically-labeled cells based on a size difference between the magnetically-labeled target cells and the magnetically-labeled non-target cells. A microfluidic device is provided including a fluidic pathway traversing a magnetic isolation region and a size-based isolation region. The magnetic isolation region includes a magnet positioned to separate magnetically-labeled cells from non-magnetically labeled cells in the magnetic isolation region. The size-based isolation region includes a separator configured to separate cells less than a threshold size from cells greater than a threshold size.