公开(公告)号：US20170268037A1

公开(公告)日：2017-09-21

申请号：US15310083

申请日：2015-05-15

Applicant: FLUXION BIOSCIENCES, INC.

Inventor： CRISTIAN IONESCU-ZANETTI ,  MICHAEL SCHWARTZ ,  CHRISTINE TING FU ,  RONG ANDREA FAN

IPC: C12Q1/68 ,  B03C1/28 ,  B03C1/01 ,  B03C1/033 ,  B01L3/00 ,  B03C1/30

CPC classification number: C12Q1/6806 ,  B01L3/502761 ,  B01L2200/0652 ,  B01L2400/043 ,  B03C1/01 ,  B03C1/0332 ,  B03C1/288 ,  B03C1/30 ,  B03C2201/18 ,  B03C2201/26 ,  C12M47/02 ,  C12N11/14 ,  G01N33/54326 ,  C12Q2563/143 ,  C12Q2563/149

Abstract: A method is provided including coupling magnetic beads to a population of cells in a fluid sample to form magnetically-labeled cells, magnetically separating the magnetically-labeled cells from non-magnetically-labeled cells in the fluid sample, and separating target cells from non-target cells of the magnetically-labeled cells based on a size difference between the magnetically-labeled target cells and the magnetically-labeled non-target cells. A microfluidic device is provided including a fluidic pathway traversing a magnetic isolation region and a size-based isolation region. The magnetic isolation region includes a magnet positioned to separate magnetically-labeled cells from non-magnetically labeled cells in the magnetic isolation region. The size-based isolation region includes a separator configured to separate cells less than a threshold size from cells greater than a threshold size.

公开(公告)号：US20160161392A1

公开(公告)日：2016-06-09

申请号：US14918329

申请日：2015-10-20

Applicant: Fluxion Biosciences, Inc.

Inventor： CRISTIAN IONESCU-ZANETTI ,  MICHELLE KHINE ,  MICHAEL SCHWARTZ

IPC: G01N15/14 ,  B01L3/00

CPC classification number: G01N15/1404 ,  B01L3/5025 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502738 ,  B01L3/502761 ,  B01L3/565 ,  B01L2200/025 ,  B01L2200/027 ,  B01L2200/0647 ,  B01L2200/0668 ,  B01L2200/0689 ,  B01L2300/041 ,  B01L2300/046 ,  B01L2300/0627 ,  B01L2300/0645 ,  B01L2300/0654 ,  B01L2300/0829 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/163 ,  B01L2400/0487 ,  B01L2400/086 ,  C12M21/06 ,  C12M23/16 ,  C12M35/04 ,  C12M41/36 ,  G01N15/1459 ,  G01N15/1463 ,  G01N15/1475 ,  G01N15/1484 ,  G01N2015/1006 ,  G01N2015/1075 ,  G01N2015/1409 ,  G01N2015/1495 ,  G01N2015/1497

Abstract: Apparatus and methods are provided for analysis of individual particles in a microfluidic device. The methods involve the immobilization of an array of particles in suspension and the application of experimental compounds. Such methods can also include electrophysiology studies including patch clamp recording, electroporation, or both in the same microfluidic device. The apparatus provided includes a microfluidic device coupled to a multi-well structure and an interface for controlling the flow of media within the microchannel device.

Abstract translation: 提供了用于分析微流体装置中的各个颗粒的装置和方法。 这些方法涉及在悬浮液中固定一系列颗粒并应用实验化合物。 这样的方法还可以包括电生理学研究，包括在同一微流体装置中的膜片钳记录，电穿孔或两者。 提供的装置包括耦合到多孔结构的微流体装置和用于控制微通道装置内的介质流动的界面。