公开(公告)号：US20240219375A1

公开(公告)日：2024-07-04

申请号：US18554103

申请日：2022-04-07

Applicant: FUJIFILM Cellular Dynamics, Inc.

Inventor： Christopher McMAHON ,  Randall LEARISH ,  Carrie CHAVEZ ,  Cayla THOMPSON

IPC: G01N33/50 ,  C12N5/0793

CPC classification number: G01N33/5058 ,  C12N5/0619 ,  G01N33/502 ,  C12N2501/119 ,  C12N2501/13 ,  C12N2501/15 ,  C12N2501/41 ,  C12N2501/415 ,  C12N2506/45 ,  G01N2500/10

Abstract: Midbrain dopaminergic neuronal precursor cells that can be used to treat a brain disorder are provided herein. Improved mono-SMAD methods are provided that can be used to differentiate pluripotent cells into midbrain dopaminergic (DA) neurons or midbrain neuronal precursors. In some aspects, methods are provided for mono-SMAD culture protocols and culture durations that can be used to generate dopaminergic neuronal precursor cells that have significantly improved properties for the treatment of a brain disorder such as, e.g., Parkinson's disease. Methods of treating Parkinson's disease and other brain diseases with the midbrain dopaminergic neuronal precursor cells are also provided.