公开(公告)号：US20230241117A1

公开(公告)日：2023-08-03

申请号：US18296652

申请日：2023-04-06

Applicant: FUJIFILM Corporation

Inventor： Kentaro NAKAMURA ,  Yuichi Yoshino ,  Atsushi Inada ,  Tsukasa Kitahashi

IPC: A61K35/28 ,  C12N5/0775

CPC classification number: A61K35/28 ,  C12N5/0668 ,  C12N2500/32 ,  C12N2500/38

Abstract: An object of the present invention is to provide a method of producing a cell preparation for a joint medical treatment, in which a cell proliferation ratio can be improved, an amount of serum required can be reduced, and karyotype abnormalities in a culture process can be suppressed, a method of culturing a mesenchymal stem cell, and a cell preparation for a joint medical treatment, which is produced by the above method. According to the present invention, there is provided a method of producing a cell preparation for a joint medical treatment, the method including culturing a mesenchymal stem cell in a culture medium containing an ascorbic acid-2-phosphate trisodium salt, alanyl glutamine, and pyridoxine.

公开(公告)号：US09932557B2

公开(公告)日：2018-04-03

申请号：US14640218

申请日：2015-03-06

Applicant: FUJIFILM Corporation

Inventor： Yuichi Yoshino ,  Rie Iwata ,  Kentaro Nakamura

IPC: C07K14/00 ,  C07K14/78 ,  A61K51/08 ,  A61K38/00 ,  C12N5/077 ,  A61L27/22

CPC classification number: C12N5/0655 ,  A61K38/00 ,  A61L27/227 ,  A61L2300/252 ,  A61L2430/06 ,  C07K14/78 ,  C12N2501/998

Abstract: A polypeptide having an amino acid sequence in which the number of RGD sequences contained per molecular weight of 10 kDa is not less than 0.30; the number of GFPGER sequences contained per molecular weight of 10 kDa is not less than 0.15; and the number of GVMGFP sequences contained per molecular weight of 10 kDa is less than 0.30; is provided. A scaffold composition, a composition for repairing a cartilage tissue, a composition for culturing cartilage cells, and a composition for promoting glycosaminoglycan production, which compositions contain the above polypeptide, are also provided.