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8 条记录 (耗时 0.032 秒)

    Male sterility restoration as a selectable marker in plant transformation
    4.
    发明申请
    Male sterility restoration as a selectable marker in plant transformation 审中-公开
    雄性不育恢复作为植物转化中的选择标记

    公开(公告)号：US20060168694A1

    公开(公告)日：2006-07-27

    申请号：US10525682

    申请日：2003-08-25

    申请人： Fang-Ming Lai ,  Benoit Landry

    发明人： Fang-Ming Lai ,  Benoit Landry

    IPC分类号： A01H1/00 ,  A01H5/00 ,  C12Q1/68 ,  C12N15/82

    CPC分类号： C12N15/8289 ,  C12N15/821

    摘要： The present invention provides methods of identifying transformed plants using the restoration of male fertility as a selectable marker. Preferably, the fertility restorer genes are used to restore fertility in Brassica napus. The present invention also includes vectors comprising male sterility genes flanked by recombinase sites, vector comprising a fertility restorer gene and a nucleotide sequence of interest flanked by recombinase sites, and methods of using such vectors to produce transgenic plants, using restoration of male fertility as the selection for transformation events.

    摘要翻译： 本发明提供使用雄性育性恢复作为选择性标记鉴定转化植物的方法。 优选地，使用育性恢复基因来恢复甘蓝型油菜的生育力。 本发明还包括包含侧翼为重组酶位点的雄性不育基因的载体，包含生育恢复基因的载体和侧翼为重组酶位点的目标核苷酸序列，以及使用这些载体产生转基因植物的方法，使用恢复雄性生育力作为 选择转型事件。

    PoC system and method for distributing media data in PoC
    5.
    发明授权
    PoC system and method for distributing media data in PoC 有权
    PoC系统和PoC中媒体数据分发方法

    公开(公告)号：US07756541B2

    公开(公告)日：2010-07-13

    申请号：US11306552

    申请日：2006-01-02

    申请人： Yien-Chang Liao ,  Fang-Ming Lai ,  Mu-Liang Wang ,  Chun-Chieh Wang

    发明人： Yien-Chang Liao ,  Fang-Ming Lai ,  Mu-Liang Wang ,  Chun-Chieh Wang

    IPC分类号： H04B7/00

    CPC分类号： H04L65/4061 ,  H04L65/4076

    摘要： The present invention provides a PoC system (push-to-talk over cellular system) and a method for distributing media data in PoC. The PoC system includes a plurality of clients, a controlling server and a first participating server. In the present invention, since a media receiving port is established in the first participating server which manages at least two clients at the same time, and when a media data is going to be distributed to the clients managed by the first participating server, the controlling server delivers the media data to the media receiving port only. In addition, a multicast addressing technology is further used in the first participating server, therefore, the path of delivering media data is changed and the efficiency of media data delivery during a communication is improved.

    摘要翻译： 本发明提供一种PoC系统（蜂窝式通话一键通）和PoC中的媒体数据分发方法。 PoC系统包括多个客户端，控制服务器和第一参与服务器。 在本发明中，由于在同时管理至少两个客户端的第一参与服务器中建立了媒体接收端口，并且当将媒体数据分发给由第一参与服务器管理的客户端时，控制 服务器仅将媒体数据传送到媒体接收端口。 此外，在第一参与服务器中进一步使用多播寻址技术，因此改变了传送媒体数据的路径，提高了通信期间媒体数据传送的效率。

    Method Of Excising A Nucleic Acid Sequence From A Plant Genome
    6.
    发明申请
    Method Of Excising A Nucleic Acid Sequence From A Plant Genome 审中-公开
    从植物基因组中切除核酸序列的方法

    公开(公告)号：US20100162428A1

    公开(公告)日：2010-06-24

    申请号：US12600902

    申请日：2008-05-30

    申请人： Jeffrey A. Brown ,  Fang-Ming Lai ,  Christina E. Roche ,  Hee-Sook Song

    发明人： Jeffrey A. Brown ,  Fang-Ming Lai ,  Christina E. Roche ,  Hee-Sook Song

    IPC分类号： A01H1/00 ,  A01H5/00

    CPC分类号： C12N15/8201 ,  C12N9/22

    摘要： The present invention relates to a method for excising a nucleic acid sequence from the genome of a plant or a plant cell. This method is based on the steps of transforming a plant cell with a construct encoding a DNA double strand break inducing enzyme (DSBI), generating a transgenic plant line, performing a transient assay to analyze the functionality of the transgenic enzyme, crossing the plant line with a line containing a nucleic acid sequence to be excised and performing an immature embryo conversion or a tissue culture regeneration through callus formation. The method can also be reversed, which means that a plant cell is transformed with a construct encoding a nucleic acid sequence to be excised, and the crossing is performed with a plant line containing a DSBI. As an alternative to the crossing step, a re-transformation of a transgenic plant line with a second construct can also be performed. The invention is also directed to a plant obtained by this method, or progeny, propagation material, part, tissue, cell or cell culture, derived from such a plant. Finally, the invention relates to the use of a plant or progeny, propagation material, part, tissue, cell or cell culture, derived from this method, as aliment, fodder or seeds or for the production of pharmaceuticals or chemicals.

    摘要翻译： 本发明涉及从植物或植物细胞的基因组中切除核酸序列的方法。 该方法基于用编码DNA双链断裂诱导酶（DSBI）的构建体转化植物细胞的步骤，产生转基因植物系，进行瞬时测定以分析转基因酶的功能，穿过植物系 含有要切除的核酸序列的线，并通过愈伤组织形成进行未成熟胚转化或组织培养再生。 该方法也可以相反，这意味着用编码待切除的核酸序列的构建体转化植物细胞，并且用含有DSBI的植物系进行杂交。 作为交叉步骤的替代方案，也可以进行具有第二构建体的转基因植物系的再转化。 本发明还涉及通过该方法获得的植物，或从这种植物衍生的后代，繁殖材料，部分，组织，细胞或细胞培养物。 最后，本发明涉及来源于该方法的植物或后代，繁殖材料，部分，组织，细胞或细胞培养物作为营养物，饲料或种子或用于生产药物或化学品的用途。

    PoC SYSTEM AND METHOD FOR DISTRIBUTING MEDIA DATA IN PoC
    8.
    发明申请
    PoC SYSTEM AND METHOD FOR DISTRIBUTING MEDIA DATA IN PoC 有权
    PoC系统和方法，用于在PoC中分发媒体数据

    公开(公告)号：US20070124492A1

    公开(公告)日：2007-05-31

    申请号：US11306552

    申请日：2006-01-02

    申请人： Yien-Chang Liao ,  Fang-Ming Lai ,  Mu-Liang Wang ,  Chun-Chieh Wang

    发明人： Yien-Chang Liao ,  Fang-Ming Lai ,  Mu-Liang Wang ,  Chun-Chieh Wang

    IPC分类号： G06F15/16

    CPC分类号： H04L65/4061 ,  H04L65/4076

    摘要： The present invention provides a PoC system (push-to-talk over cellular system) and a method for distributing media data in PoC. The PoC system includes a plurality of clients, a controlling server and a first participating server. In the present invention, since a media receiving port is established in the first participating server which manages at least two clients at the same time, and when a media data is going to be distributed to the clients managed by the first participating server, the controlling server delivers the media data to the media receiving port only. In addition, a multicast addressing technology is further used in the first participating server, therefore, the path of delivering media data is changed and the efficiency of media data delivery during a communication is improved.

    摘要翻译： 本发明提供一种PoC系统（蜂窝式通话一键通）和PoC中的媒体数据分发方法。 PoC系统包括多个客户端，控制服务器和第一参与服务器。 在本发明中，由于在同时管理至少两个客户端的第一参与服务器中建立了媒体接收端口，并且当将媒体数据分发给由第一参与服务器管理的客户端时，控制 服务器仅将媒体数据传送到媒体接收端口。 此外，在第一参与服务器中进一步使用多播寻址技术，因此改变了传送媒体数据的路径，提高了通信期间媒体数据传送的效率。