公开(公告)号：US07238356B2

公开(公告)日：2007-07-03

申请号：US10128590

申请日：2002-04-24

申请人： Fons Bosman ,  Erik Depla ,  Geert Deschamps ,  Erwin Sablon ,  Manfred Suckow ,  Isabelle Samson ,  Gert Verheyden

发明人： Fons Bosman ,  Erik Depla ,  Geert Deschamps ,  Erwin Sablon ,  Manfred Suckow ,  Isabelle Samson ,  Gert Verheyden

IPC分类号： A61K39/29 ,  A61K48/00 ,  C12P21/04

CPC分类号： C07K14/005 ,  A61K38/00 ,  A61K39/00 ,  A61K2039/5258 ,  C07K2319/02 ,  C07K2319/50 ,  C12N7/00 ,  C12N2770/24222 ,  C12N2770/24223

摘要： The current invention relates to HCV envelope proteins or parts thereof which are the product of expression in eukaryotic cells. More particularly said HCV envelope proteins are characterized in that on average up to 80% of their N-glycosylation sites are core-glycosylated. Of these N-glycosylated sites more than 70% are glycosylated with an oligomannose having a structure defined by Man(8 to 10)-GlcNAc(2). Furthermore, the ratio of the oligomannose with structure Man(7)-GlcNAc(2) over the oligomannose with structure Man(8)-GlcNAc(2) is less than or equal to 0.45. Less than 10% of the oligomannoses is terminated with an α1,3 linked mannose. The HCV envelope proteins of the invention are particularly suited for diagnostic, prophylactic and therapeutic purposes. A suitable eukaryotic cell for production of the HCV envelope proteins of the invention is a Hansenula cell.

摘要翻译： 本发明涉及作为在真核细胞中表达的产物的HCV包膜蛋白或其部分。 更具体地说，HCV包膜蛋白的特征在于平均高达80％的N-糖基化位点是核糖基化的。 在这些N-糖基化位点中，大于70％的N-糖基化位点用具有由Man（8至10）-GlcNAc（2）定义的结构的寡甘露糖糖基化。 此外，结构Man（7）-GlcNAc（2）的寡甘露糖与结构Man（8）-GlcNAc（2）的寡甘露糖的比例小于或等于0.45。 少于10％的寡甘露聚糖用α1,3连接的甘露糖终止。 本发明的HCV包膜蛋白特别适用于诊断，预防和治疗目的。 用于生产本发明的HCV包膜蛋白的合适的真核细胞是汉逊酵母细胞。

公开(公告)号：US07718398B2

公开(公告)日：2010-05-18

申请号：US10513836

申请日：2003-05-08

申请人： Manfred Suckow

发明人： Manfred Suckow

IPC分类号： C12P21/02

CPC分类号： C12N15/815 ,  C07K14/39

摘要： The present invention relates to a deoxyribonucleic acid (DNA) comprising at least one promoter sequence, which is derived from a wild-type promoter of a methyltrophic yeast, whose transcription efficiency is modulated in comparison to the efficiency of the wild-type promoter by inserting or modifying a DNA binding site. The invention also relates to host cells, expression vectors, kits and methods for producing proteins while using the inventive DNA, as well as to different uses of the same and to a method for producing expression vectors.

摘要翻译： 本发明涉及包含至少一个启动子序列的脱氧核糖核酸（DNA），该启动子序列衍生自甲基营养酵母的野生型启动子，其转录效率与野生型启动子的效率相比是通过插入 或修饰DNA结合位点。 本发明还涉及宿主细胞，表达载体，试剂盒和用于在使用本发明的DNA时产生蛋白质的方法，以及它们的不同用途和生产表达载体的方法。

公开(公告)号：US20060089492A1

公开(公告)日：2006-04-27

申请号：US10513836

申请日：2003-05-08

申请人： Manfred Suckow

发明人： Manfred Suckow

IPC分类号： C07K14/39 ,  C07H21/04 ,  C12P21/06 ,  C12N1/18 ,  C12N15/74

CPC分类号： C12N15/815 ,  C07K14/39

摘要： The present invention relates to a deoxyribonucleic acid (DNA) comprising at least one promoter sequence, which is derived from a wild-type promoter of a methyltrophic yeast, whose transcription efficiency is modulated in comparison to the efficiency of the wild-type promoter by inserting or modifying a DNA binding site. The invention also relates to host cells, expression vectors, kits and methods for producing proteins while using the inventive DNA, as well as to different uses of the same and to a method for producing expression vectors.

摘要翻译： 本发明涉及包含至少一个启动子序列的脱氧核糖核酸（DNA），该启动子序列衍生自甲基营养酵母的野生型启动子，其转录效率与野生型启动子的效率相比是通过插入 或修饰DNA结合位点。 本发明还涉及宿主细胞，表达载体，试剂盒和用于在使用本发明的DNA时产生蛋白质的方法，以及它们的不同用途和生产表达载体的方法。