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    Preparative separation of mixtures by mass spectrometry
    1.
    发明授权
    Preparative separation of mixtures by mass spectrometry 失效
    通过质谱法制备混合物分离

    公开(公告)号：US06750448B2

    公开(公告)日：2004-06-15

    申请号：US10386127

    申请日：2003-03-10

    申请人： Frantisek Turecek ,  Adi Scheidemann ,  Terry Olney ,  Frank J. Schumacher ,  Martin Smrcina ,  Peter Strop ,  Marcel Patek ,  Daniel Schirlin

    发明人： Frantisek Turecek ,  Adi Scheidemann ,  Terry Olney ,  Frank J. Schumacher ,  Martin Smrcina ,  Peter Strop ,  Marcel Patek ,  Daniel Schirlin

    IPC分类号： H01J4930

    CPC分类号： H01J49/0086

    摘要： The present invention provides an instrument and methods for the preparative separation of components of mixtures using mass spectrometric methods. Nondestructive ionization methods are employed to generate ionized components of a mixture, the ionized components are spatially separated by mass and the mass-separated ion components are trapped. The ion source and mass spectrometric techniques employed allow the generation of large ion currents of ion components, on the order of nanoamps, which facilitate rapid accumulation of nanomole quantities of mass-separated components in relatively short times (minutes to hours).

    摘要翻译： 本发明提供了使用质谱法制备分离混合物组分的仪器和方法。 采用非破坏性电离方法产生混合物的离子化成分，离子化成分在空间上被质量分离，质量分离的离子成分被捕获。 使用的离子源和质谱技术允许产生大约数量级的离子组分的大离子电流，这有助于在相当短的时间（几分钟至几小时）内快速积累纳摩尔量的质量分离组分。

    Rapid quantitative analysis of proteins or protein function in complex mixtures
    3.
    发明授权
    Rapid quantitative analysis of proteins or protein function in complex mixtures 有权
    蛋白质或蛋白质功能在复杂混合物中的快速定量分析

    公开(公告)号：US06670194B1

    公开(公告)日：2003-12-30

    申请号：US09383062

    申请日：1999-08-25

    申请人： Rudolf Hans Aebersold ,  Michael H. Gelb ,  Steven P. Gygi ,  C. Ronald Scott ,  Frantisek Turecek ,  Scott A. Gerber ,  Beate Rist

    发明人： Rudolf Hans Aebersold ,  Michael H. Gelb ,  Steven P. Gygi ,  C. Ronald Scott ,  Frantisek Turecek ,  Scott A. Gerber ,  Beate Rist

    IPC分类号： C07C33116

    CPC分类号： G01N33/6848 ,  C12Q1/25 ,  G01N33/58 ,  G01N33/6803 ,  G01N33/6842 ,  Y10S530/812 ,  Y10T436/182 ,  Y10T436/24 ,  Y10T436/25 ,  Y10T436/25125 ,  Y10T436/25375

    摘要： Analytical reagents and mass spectrometry-based methods using these reagents for the rapid, and quantitative analysis of proteins or protein function in mixtures of proteins. The methods employ affinity labeled protein reactive reagents having three portions: an affinity label (A) covalently linked to a protein reactive group (PRG) through a linker group (L). The linker may be differentially isotopically labeled, e.g., by substitution of one or more atoms in the linker with a stable isotope thereof. These reagents allow for the selective isolation of peptide fragments or the products of reaction with a given protein (e.g., products of enzymatic reaction) from complex mixtures. The isolated peptide fragments or reaction products are characteristic of the presence of a protein or the presence of a protein function in those mixtures. Isolated peptides or reaction products are characterized by mass spectrometric (MS) techniques. The reagents also provide for differential isotopic labeling of the isolated peptides or reaction products which facilitates quantitative determination by mass spectrometry of the relative amounts of proteins in different samples. The methods of this invention can be used for qualitative and quantitative analysis of global protein expression profiles in cells and tissues, to screen for and identify proteins whose expression level in cells, tissue or biological fluids is affected by a stimulus or by a change in condition or cell state of the cell, tissue or organism from which the sample originated.

    摘要翻译： 使用这些试剂的分析试剂和基于质谱的方法用于蛋白质混合物中蛋白质或蛋白质功能的快速和定量分析。 该方法采用具有三个部分的亲和标记的蛋白质反应试剂：通过连接基团（L）与蛋白质反应基团（PRG）共价连接的亲和标记（A）。 连接体可以是差异同位素标记的，例如用连接体中的一个或多个原子用其稳定同位素取代。 这些试剂允许从复杂混合物中选择性分离肽片段或与给定蛋白质（例如，酶反应产物）反应的产物。 分离的肽片段或反应产物是这些混合物中存在蛋白质或存在蛋白质功能的特征。 分离的肽或反应产物的特征在于质谱（MS）技术。 试剂还提供分离的肽或反应产物的差异同位素标记，其有助于通过质谱法定量测定不同样品中蛋白质的相对量。 本发明的方法可用于细胞和组织中全球蛋白质表达谱的定性和定量分析，筛选和鉴定细胞，组织或生物流体中的表达水平受刺激或病情变化影响的蛋白质 或来自样品的细胞，组织或生物体的细胞状态。

    Reagent and method for detection of carboxylic acids by mass spectrometry
    4.
    发明授权
    Reagent and method for detection of carboxylic acids by mass spectrometry 有权
    通过质谱法检测羧酸的试剂和方法

    公开(公告)号：US09045421B2

    公开(公告)日：2015-06-02

    申请号：US13078652

    申请日：2011-04-01

    申请人： Michael H. Gelb ,  Frantisek Turecek ,  James Bollinger

    发明人： Michael H. Gelb ,  Frantisek Turecek ,  James Bollinger

    IPC分类号： C07D211/70 ,  C07D211/82 ,  C07D213/00 ,  C07D211/92 ,  C07D213/18 ,  C07D213/20 ,  G01N33/88 ,  G01N33/92 ,  H01J49/16

    CPC分类号： C07D213/20 ,  G01N33/88 ,  G01N33/92 ,  H01J49/165 ,  Y10T436/174614

    摘要： Method and reagent for converting a carboxylic acid to a positively charge amide are described. The method and reagent facilitate positive ion mass spectral analysis of carboxylic acids. The method includes reacting a carboxylic acid with a compound having formula I: wherein A and B are aromatic rings, ring A includes a quaternized nitrogen and has n additional ring atoms, each additional ring atom optionally substituted with an RA group, and n is an integer from 4 to 10, and ring B includes a carbon atom and has m additional ring atoms, each additional ring atom optionally substituted with an RB group, and m is an integer from 4 to 10. The compound includes at least one RA or RB group, and the at least one RA and RB group is -L-N(Z)H; and X− is a counterion.

    摘要翻译： 描述了将羧酸转化为正电荷酰胺的方法和试剂。 该方法和试剂促进了羧酸的正离子质谱分析。 该方法包括使羧酸与具有式I的化合物反应：其中A和B是芳环，环A包括季铵化的氮并具有n个另外的环原子，每个另外的环原子任选地被RA基团取代，n是 4〜10的整数，环B包含碳原子并具有另外的环原子，每个另外的环原子任选被一个RB基团取代，m是4-10的整数。该化合物包括至少一个RA或RB 并且所述至少一个RA和RB基团是-LN（Z）H; 而X-是抗衡离子。

    Rapid quantitative analysis of proteins or protein function in complex mixtures
    5.
    发明授权
    Rapid quantitative analysis of proteins or protein function in complex mixtures 有权
    蛋白质或蛋白质功能在复杂混合物中的快速定量分析

    公开(公告)号：US06852544B2

    公开(公告)日：2005-02-08

    申请号：US09839884

    申请日：2001-04-20

    申请人： Rudolf Hans Aebersold ,  Michael H. Gelb ,  Steven P. Gygi ,  C. Ronald Scott ,  Frantisek Turecek ,  Scott A. Gerber ,  Beate Rist

    发明人： Rudolf Hans Aebersold ,  Michael H. Gelb ,  Steven P. Gygi ,  C. Ronald Scott ,  Frantisek Turecek ,  Scott A. Gerber ,  Beate Rist

    IPC分类号： G01N27/62 ,  C12Q1/25 ,  C12Q1/34 ,  C12Q1/48 ,  G01N30/00 ,  G01N30/06 ,  G01N30/60 ,  G01N30/72 ,  G01N30/88 ,  G01N33/53 ,  G01N33/566 ,  G01N33/58 ,  G01N33/68

    CPC分类号： G01N33/6848 ,  C12Q1/25 ,  G01N33/58 ,  G01N33/6803 ,  G01N33/6842 ,  Y10S530/812 ,  Y10T436/182 ,  Y10T436/24 ,  Y10T436/25 ,  Y10T436/25125 ,  Y10T436/25375

    摘要： Analytical reagents and mass spectrometry-based methods using these reagents for the rapid, and quantitative analysis of proteins or protein function in mixtures of proteins. The methods employ affinity labeled protein reactive reagents having three portions: an affinity label (A) covalently linked to a protein reactive group (PRG) through a linker group (L). The linker may be differentially isotopically labeled, e.g., by substitution of one or more atoms in the linker with a stable isotope thereof. These reagents allow for the selective isolation of peptide fragments or the products of reaction with a given protein (e.g., products of enzymatic reaction) from complex mixtures. The isolated peptide fragments or reaction products are characteristic of the presence of a protein or the presence of a protein function in those mixtures. Isolated peptides or reaction products are characterized by mass spectrometric (MS) techniques. The reagents also provide for differential isotopic labeling of the isolated peptides or reaction products which facilitates quantitative determination by mass spectrometry of the relative amounts of proteins in different samples. The methods of this invention can be used for qualitative and quantitative analysis of global protein expression profiles in cells and tissues, to screen for and identify proteins whose expression level in cells, tissue or biological fluids is affected by a stimulus or by a change in condition or state of the cell, tissue or organism from which the sample originated.

    摘要翻译： 使用这些试剂的分析试剂和基于质谱的方法用于蛋白质混合物中蛋白质或蛋白质功能的快速和定量分析。 该方法采用具有三个部分的亲和标记的蛋白质反应试剂：通过连接基团（L）与蛋白质反应基团（PRG）共价连接的亲和标记（A）。 连接体可以是差异同位素标记的，例如用连接体中的一个或多个原子用其稳定同位素取代。 这些试剂允许从复杂混合物中选择性分离肽片段或与给定蛋白质（例如，酶反应产物）反应的产物。 分离的肽片段或反应产物是这些混合物中存在蛋白质或存在蛋白质功能的特征。 分离的肽或反应产物的特征在于质谱（MS）技术。 试剂还提供分离的肽或反应产物的差异同位素标记，其有助于通过质谱法定量测定不同样品中蛋白质的相对量。 本发明的方法可用于细胞和组织中全球蛋白质表达谱的定性和定量分析，筛选和鉴定细胞，组织或生物流体中的表达水平受刺激或病情变化影响的蛋白质 或来自样品的细胞，组织或生物体的状态。

    Chemical reagents and methods for detection and quantification of proteins in complex mixtures
    6.
    发明授权
    Chemical reagents and methods for detection and quantification of proteins in complex mixtures 有权
    用于复杂混合物中蛋白质的检测和定量的化学试剂和方法

    公开(公告)号：US07473535B2

    公开(公告)日：2009-01-06

    申请号：US10225447

    申请日：2002-08-20

    申请人： Rudolf H. Aebersold ,  Patricia Q. Bottari ,  Michael H. Gelb ,  Frantisek Turecek

    发明人： Rudolf H. Aebersold ,  Patricia Q. Bottari ,  Michael H. Gelb ,  Frantisek Turecek

    IPC分类号： G01N33/53 ,  A61K38/00 ,  C07K1/00

    CPC分类号： G01N33/585 ,  G01N33/6803 ,  G01N33/6809

    摘要： The invention provides a reagent comprising an affinity tag, a detectable moiety, a linker, an isotope tag and a reactive group. The invention also provides methods of using a reagent of the invention. The methods can be used to label a polypeptide in a sample by contacting a sample with a reagent of the invention under conditions allowing the reactive group to bind to one or more polypeptides in the sample. The invention additionally provides methods of isolating, identifying and quantifying a polypeptide in a sample. The invention further provides methods of diagnosing a disease using a reagent of the invention.

    摘要翻译： 本发明提供了包含亲和标签，可检测部分，接头，同位素标签和反应性基团的试剂。 本发明还提供了使用本发明试剂的方法。 该方法可以用于在样品中通过使样品与本发明的试剂在允许反应性基团与样品中的一种或多种多肽结合的条件下接触来标记样品中的多肽。 本发明另外提供了分离，鉴定和定量样品中的多肽的方法。 本发明还提供使用本发明的试剂诊断疾病的方法。